{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:45:48Z","timestamp":1767926748633,"version":"3.49.0"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The QingGan LiDan capsule (QGLD) consists of five traditional Chinese herbs, which have been used for hepatobiliary diseases such as jaundice. However, the effects and mechanisms by which QGLD prevent alcoholic liver diseases (ALD) remain unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim of the study<\/jats:title><jats:p>Investigate the therapeutic potential of QingGan Lidan capsule (QGLD) in alleviating alcohol-induced liver injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Acute alcoholic liver injury model and chronic and Binge ethanol Feeding Model (NIAAA) model were established. Mice were administered QGLD (360, 720, 1,440\u00a0mg\/kg) or vehicle. Liver function indicators (ALT, AST), serum lipid (TC, TG), antioxidant markers (SOD, GSH, MDA), lipid metabolism\/transport genes relative expression levels, liver and ileal villus morphology were analyzed. Network pharmacology analysis was also performed to identify potential targets and pathways of QGLD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>QGLD reduced serum ALT, AST, hepatic TC, TG, and lipid droplet accumulation in both models. It upregulated antioxidant enzymes (SOD, GSH) and downregulated MDA. QGLD regulated the mRNA levels of genes related to the NRF2\/KEAP1 pathway and lipid transport. Network pharmacology identified 221 potential targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>QGLD mitigates alcohol-induced liver injury by reducing lipid accumulation, regulating lipid transport and enhancing antioxidant capacity. This supports its potential application in ALD management.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fphar.2025.1575280","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T08:41:17Z","timestamp":1742978477000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["QingGan LiDan capsules improved alcoholic liver injury by regulating liver lipid transport and oxidative stress in mice"],"prefix":"10.3389","volume":"16","author":[{"given":"Zhiwen","family":"Fu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiafeng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongye","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenzhen","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujia","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianbiao","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangning","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1038\/nprot.2013.032","article-title":"Mouse model of chronic and binge ethanol feeding (the NIAAA model)","volume":"8","author":"Bertola","year":"2013","journal-title":"Nat. Protoc."},{"key":"B2","doi-asserted-by":"publisher","first-page":"112727","DOI":"10.1016\/j.biopha.2022.112727","article-title":"Autophagy and cancer: can tetrandrine be a potent anticancer drug in the near future?","volume":"148","author":"Bhagya","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.3390\/nu15071571","article-title":"The influence of alcohol consumption on intestinal nutrient absorption: a comprehensive review","volume":"15","author":"Butts","year":"2023","journal-title":"Nutrients"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1002","DOI":"10.3390\/antiox12051002","article-title":"Dragon fruit peel waste (hylocereus undatus) as a potential ingredient for reducing lipid peroxidation, dietary advanced glycation end products, and starch digestibility in cookies","volume":"12","author":"Chumroenvidhayakul","year":"2023","journal-title":"Antioxidants (Basel)"},{"key":"B5","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1002\/biof.1718","article-title":"Powerful anti-inflammatory action of luteolin: potential increase with IL-38","volume":"47","author":"Conti","year":"2021","journal-title":"Biofactors"},{"key":"B6","doi-asserted-by":"publisher","first-page":"e65325","DOI":"10.7554\/eLife.65325","article-title":"Alcohol consumption in the general population is associated with structural changes in multiple organ systems","volume":"10","author":"Evangelou","year":"2021","journal-title":"Elife"},{"key":"B7","doi-asserted-by":"publisher","first-page":"205","DOI":"10.14715\/cmb\/2024.70.2.29","article-title":"Tetrandrine alleviates inflammation and promotes macrophage M2 polarization in gouty arthritis by NF-\u03baB-mediated Lcp1","volume":"70","author":"Fang","year":"2024","journal-title":"Cell Mol. Biol. (Noisy-le-grand)"},{"key":"B8","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/j.ecl.2022.02.008","article-title":"Lipid and lipoprotein metabolism","volume":"51","author":"Feingold","year":"2022","journal-title":"Endocrinol. Metab. Clin. North Am."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1238","DOI":"10.1038\/s41401-022-01028-9","article-title":"Scoparone suppresses mitophagy-mediated NLRP3 inflammasome activation in inflammatory diseases","volume":"44","author":"Feng","year":"2023","journal-title":"Acta Pharmacol. Sin."},{"key":"B10","doi-asserted-by":"publisher","first-page":"100530","DOI":"10.1016\/j.jlr.2024.100530","article-title":"Apolipoprotein A1 and high-density lipoprotein limit low-density lipoprotein transcytosis by binding SR-B1","volume":"65","author":"Fung","year":"2024","journal-title":"J. Lipid Res."},{"key":"B11","doi-asserted-by":"publisher","first-page":"173","DOI":"10.3727\/105221617X695519","article-title":"Animal models of alcoholic liver disease: pathogenesis and clinical relevance","volume":"17","author":"Gao","year":"2017","journal-title":"Gene Expr."},{"key":"B12","doi-asserted-by":"publisher","first-page":"3044","DOI":"10.1002\/jcp.30083","article-title":"Scoparone alleviates hepatic fibrosis by inhibiting the TLR-4\/NF-\u03baB pathway","volume":"236","author":"Gao","year":"2020","journal-title":"J. Cell Physiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1953","DOI":"10.3390\/antiox12111953","article-title":"The key role of GSH in keeping the redox balance in mammalian cells: mechanisms and significance of GSH in detoxification via formation of conjugates","volume":"12","author":"Georgiou-Siafis","year":"2023","journal-title":"Antioxidants (Basel)"},{"key":"B14","doi-asserted-by":"publisher","first-page":"10","DOI":"10.31083\/j.fbl2801010","article-title":"Cellular uptake, metabolism and sensing of long-chain fatty acids","volume":"28","author":"He","year":"2023","journal-title":"Front. Biosci. Landmark Ed."},{"key":"B15","doi-asserted-by":"publisher","first-page":"1148827","DOI":"10.3389\/fendo.2023.1148827","article-title":"Chronic-binge ethanol feeding aggravates systemic dyslipidemia in Ldlr(-\/-) mice, thereby accelerating hepatic fibrosis","volume":"14","author":"Hoebinger","year":"2023","journal-title":"Front. Endocrinol. (Lausanne)"},{"key":"B16","doi-asserted-by":"publisher","first-page":"177033","DOI":"10.1016\/j.ejphar.2024.177033","article-title":"Scoparone alleviates nonalcoholic fatty liver disease by modulating the PPAR\u03b1 signaling pathway","volume":"984","author":"Huang","year":"2024","journal-title":"Eur. J. Pharmacol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"101178","DOI":"10.1016\/j.plipres.2022.101178","article-title":"Intestinal fatty acid binding protein: a rising therapeutic target in lipid metabolism","volume":"87","author":"Huang","year":"2022","journal-title":"Prog. Lipid Res."},{"key":"B18","doi-asserted-by":"publisher","first-page":"5717","DOI":"10.3390\/ijms22115717","article-title":"Pathophysiological aspects of alcohol metabolism in the liver","volume":"22","author":"Hyun","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"B19","doi-asserted-by":"publisher","first-page":"E1183","DOI":"10.1152\/ajpendo.90899.2008","article-title":"Intestinal lipid absorption","volume":"296","author":"Iqbal","year":"2009","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"B20","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1194\/jlr.R119000547","article-title":"Alcohol effects on hepatic lipid metabolism","volume":"61","author":"Jeon","year":"2020","journal-title":"J. Lipid Res."},{"key":"B21","doi-asserted-by":"publisher","first-page":"863756","DOI":"10.3389\/fphar.2022.863756","article-title":"Scoparone improves nonalcoholic steatohepatitis through alleviating JNK\/sab signaling pathway-mediated mitochondrial dysfunction","volume":"13","author":"Jiang","year":"2022","journal-title":"Front. Pharmacol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"121202","DOI":"10.1016\/j.lfs.2022.121202","article-title":"Anti-nociceptive effects of magnolol via inhibition of TRPV1\/P2Y and TLR4\/NF-\u03baB signaling in a postoperative pain model","volume":"312","author":"Khan","year":"2023","journal-title":"Life Sci."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1885","DOI":"10.3390\/antiox12101885","article-title":"Combination of cysteine and glutathione prevents ethanol-induced hangover and liver damage by modulation of Nrf2 signaling in HepG2 cells and mice","volume":"12","author":"Kim","year":"2023","journal-title":"Antioxidants (Basel)"},{"key":"B24","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1152\/physrev.00041.2018","article-title":"Free fatty acid receptors in health and disease","volume":"100","author":"Kimura","year":"2020","journal-title":"Physiol. Rev."},{"key":"B25","doi-asserted-by":"publisher","first-page":"5063","DOI":"10.3748\/wjg.v24.i45.5063","article-title":"Alcoholic liver disease: utility of animal models","volume":"24","author":"Lamas-Paz","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.2174\/0115680266282360240222062032","article-title":"Tetrandrine for targeting therapy resistance in cancer","volume":"24","author":"Lima","year":"2024","journal-title":"Curr. Top. Med. Chem."},{"key":"B27","doi-asserted-by":"publisher","first-page":"632767","DOI":"10.3389\/fphar.2021.632767","article-title":"Pharmacology, toxicity, bioavailability, and formulation of magnolol: an update","volume":"12","author":"Lin","year":"2021","journal-title":"Front. Pharmacol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"109895","DOI":"10.1016\/j.biopha.2020.109895","article-title":"Scoparone improves hepatic inflammation and autophagy in mice with nonalcoholic steatohepatitis by regulating the ROS\/P38\/Nrf2 axis and PI3K\/AKT\/mTOR pathway in macrophages","volume":"125","author":"Liu","year":"","journal-title":"Biomed. Pharmacother."},{"key":"B29","doi-asserted-by":"publisher","first-page":"104631","DOI":"10.1016\/j.phrs.2020.104631","article-title":"Geniposide reduces cholesterol accumulation and increases its excretion by regulating the FXR-mediated liver-gut crosstalk of bile acids","volume":"152","author":"Liu","year":"","journal-title":"Pharmacol. Res."},{"key":"B30","doi-asserted-by":"publisher","first-page":"3944","DOI":"10.1080\/21655979.2022.2031396","article-title":"Tetrandrine reduces oxidative stress, apoptosis, and extracellular matrix degradation and improves intervertebral disc degeneration by inducing autophagy","volume":"13","author":"Liu","year":"2022","journal-title":"Bioengineered"},{"key":"B31","doi-asserted-by":"publisher","first-page":"3136","DOI":"10.1111\/jcmm.16304","article-title":"Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress","volume":"25","author":"Lyu","year":"2021","journal-title":"J. Cell Mol. Med."},{"key":"B32","doi-asserted-by":"publisher","first-page":"e202300754","DOI":"10.1002\/cbdv.202300754","article-title":"Research progress on the structural modification of magnolol and honokiol and the biological activities of their derivatives","volume":"20","author":"Ming-Xin Guo","year":"2023","journal-title":"Chem. Biodivers."},{"key":"B33","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1007\/s12272-022-01392-4","article-title":"Alcoholic liver disease: a new insight into the pathogenesis of liver disease","volume":"45","author":"Park","year":"2022","journal-title":"Arch. Pharm. Res."},{"key":"B34","volume-title":"Pharmacopoeia of people's Republic of China","author":"Pharmacopoeia","year":"2020"},{"key":"B35","doi-asserted-by":"publisher","first-page":"15995","DOI":"10.3390\/ijms242115995","article-title":"Application of luteolin in neoplasms and nonneoplastic diseases","volume":"24","author":"Rakoczy","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1425","DOI":"10.3390\/antiox12071425","article-title":"Autophagy, oxidative stress, and alcoholic liver disease: a systematic review and potential clinical applications","volume":"12","author":"Salete-Granado","year":"2023","journal-title":"Antioxidants (Basel)"},{"key":"B37","doi-asserted-by":"publisher","first-page":"5097","DOI":"10.1111\/jcmm.15139","article-title":"Geniposide alleviates non-alcohol fatty liver disease via regulating Nrf2\/AMPK\/mTOR signalling pathways","volume":"24","author":"Shen","year":"2020","journal-title":"J. Cell Mol. Med."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1274","DOI":"10.1038\/s41401-021-00693-6","article-title":"Tetrandrine alleviates silicosis by inhibiting canonical and non-canonical NLRP3 inflammasome activation in lung macrophages","volume":"43","author":"Song","year":"2022","journal-title":"Acta Pharmacol. Sin."},{"key":"B39","doi-asserted-by":"publisher","first-page":"4660","DOI":"10.3748\/wjg.v30.i43.4660","article-title":"Elafibranor alleviates alcohol-related liver fibrosis by restoring intestinal barrier function","volume":"30","author":"Sun","year":"2024","journal-title":"World J. Gastroenterol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1856","DOI":"10.1136\/gutjnl-2021-324295","article-title":"Clinical, histological and molecular profiling of different stages of alcohol-related liver disease","volume":"71","author":"Ventura-Cots","year":"2022","journal-title":"Gut"},{"key":"B41","doi-asserted-by":"publisher","first-page":"155055","DOI":"10.1016\/j.phymed.2023.155055","article-title":"Kaempferol ameliorated alcoholic liver disease through inhibiting hepatic bile acid synthesis by targeting intestinal FXR-FGF15 signaling","volume":"120","author":"Xiao","year":"2023","journal-title":"Phytomedicine"},{"key":"B42","doi-asserted-by":"publisher","first-page":"154961","DOI":"10.1016\/j.phymed.2023.154961","article-title":"Chlorogenic acids: a pharmacological systematic review on their hepatoprotective effects","volume":"118","author":"Xue","year":"2023","journal-title":"Phytomedicine"},{"key":"B43","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1186\/s12967-023-04166-8","article-title":"Pathogenic mechanisms and regulatory factors involved in alcoholic liver disease","volume":"21","author":"Yan","year":"2023","journal-title":"J. Transl. Med."},{"key":"B44","doi-asserted-by":"publisher","first-page":"122714","DOI":"10.1016\/j.lfs.2024.122714","article-title":"PIM1 alleviated liver oxidative stress and NAFLD by regulating the NRF2\/HO-1\/NQO1 pathway","volume":"349","author":"Yang","year":"2024","journal-title":"Life Sci."},{"key":"B45","doi-asserted-by":"publisher","first-page":"772635","DOI":"10.3389\/fphar.2021.772635","article-title":"Geniposide ameliorates liver fibrosis through reducing oxidative stress and inflammatory respose, inhibiting apoptosis and modulating overall metabolism","volume":"12","author":"Yang","year":"2021","journal-title":"Front. Pharmacol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"115943","DOI":"10.1016\/j.jep.2022.115943","article-title":"Integration of pharmacodynamics, network pharmacology and metabolomics to elucidate effect and mechanism of Artemisia capillaris Thunb. in the treatment of jaundice","volume":"303","author":"Yang","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1161\/CIRCRESAHA.123.322607","article-title":"Chylomicrons regulate lacteal permeability and intestinal lipid absorption","volume":"133","author":"Zarkada","year":"2023","journal-title":"Circ. Res."},{"key":"B48","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.3390\/antiox12091677","article-title":"The effect of preventing oxidative stress and its mechanisms in the extract from Sonchus brachyotus DC. Based on the Nrf2-Keap1-ARE signaling pathway","volume":"12","author":"Zhang","year":"2023","journal-title":"Antioxidants (Basel)"}],"container-title":["Frontiers in Pharmacology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2025.1575280\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T08:41:30Z","timestamp":1742978490000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2025.1575280\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,26]]},"references-count":48,"alternative-id":["10.3389\/fphar.2025.1575280"],"URL":"https:\/\/doi.org\/10.3389\/fphar.2025.1575280","relation":{},"ISSN":["1663-9812"],"issn-type":[{"value":"1663-9812","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,26]]},"article-number":"1575280"}}