{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T21:59:19Z","timestamp":1793397559058,"version":"4.5.0"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T00:00:00Z","timestamp":1761264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Scorpion and Centipede (SC) is an ancient formula of traditional Chinese medicine that is commonly utilized in a range of disorders, and it has been shown to have pharmacological effects on postmenopausal osteoporosis (PMOP). However, the specific mechanism of SC for the treatment of PMOP remains to be further investigated. This study aimed to investigate the therapeutic potential of a traditional Chinese medicine formula consisting of SC in regulating the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) to treat PMOP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The ovariectomy-induced mice (OVX) were established and divided into a sham surgery group, OVX, OVX with alendronate sodium, and OVX with SC, and kept for 10 weeks.\n                      <jats:italic>In vitro<\/jats:italic>\n                      experiments were conducted to evaluate the effects of SC on osteogenic and adipogenic differentiation in BMMSCs, MC3T3-E1, and 3T3-L1 cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results showed that SC treatment significantly improved bone mineral density (BMD), trabecular separation (Tb.Sp), trabecular thickness (Tb.Th), trabecular number (Tb.N), bone volume fraction (BV\/TV), and trabecular pattern factor (Tb.Pf) in OVX mice. In addition, SC treatment markedly increased Runx2, Osx, Alp, and Cx3cr1 while decreasing adipogenic genes like PPAR\u03b3 and C\/EBP\u03b1 in the bone tissue of OVX mice and BMMSCs. Notably, the effects of SC on osteogenic and adipogenic genes were blocked in Cx3cr1 knockdown MC3T3-E1 and 3T3-L1 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study demonstrates that the SC effectively increases bone mass and osteogenesis by promoting Cx3cr1, thereby increasing osteogenic differentiation and inhibiting adipogenic differentiation. These findings amplified the mechanisms of SC and its potential to treat PMOP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2025.1604096","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T05:28:17Z","timestamp":1761283697000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Scorpion-Centipede extracts mitigate ovariectomy-induced osteoporosis in mice through facilitating Cx3cr1 expression"],"prefix":"10.3389","volume":"16","author":[{"given":"Jinghuai","family":"Ni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingjie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbin","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penghua","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Min","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,10,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.20517\/mtod.2023.38","article-title":"Clinical frontiers of metabolic bone disorders: a comprehensive review","volume":"4","author":"Ansari","year":"2023","journal-title":"Metabolism Target Organ Damage"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1007\/s40265-015-0417-7","article-title":"Modulating bone resorption and bone formation in opposite directions in the treatment of postmenopausal osteoporosis","volume":"75","author":"Appelman-Dijkstra","year":"2015","journal-title":"Drugs"},{"key":"B3","doi-asserted-by":"publisher","first-page":"544","DOI":"10.3803\/EnM.2021.301","article-title":"Long-term treatment of postmenopausal osteoporosis","volume":"36","author":"Brown","year":"2021","journal-title":"Endocrinol. 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