{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T06:22:36Z","timestamp":1787898156380,"version":"build-2784847793"},"reference-count":59,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>Chronic inflammation and oxidative stress are pivotal drivers of pathological conditions, necessitating safer plant-derived therapeutic alternatives. This study elucidates the anti-inflammatory and antioxidant mechanisms of <jats:italic>Nymphaea<\/jats:italic> \u201cEldorado\u201d flower water extract (NEWE) in a zebrafish (<jats:italic>Danio rerio<\/jats:italic>) model of copper sulfate (CuSO<jats:sub>4<\/jats:sub>)-induced inflammation. NEWE (25\u2013100\u00a0\u03bcg\/mL) attenuates CuSO<jats:sub>4<\/jats:sub>-triggered neutrophil migration and oxidative stress by downregulating proinflammatory genes (<jats:italic>il1b<\/jats:italic>, <jats:italic>ptgs2a\/b<\/jats:italic>) and reducing reactive oxygen species (ROS) production. Transcriptomic profiling identified 339 differentially expressed genes (DEGs), enriched in cytokine signaling and redox regulation, with <jats:italic>serpine1<\/jats:italic>, <jats:italic>stat3<\/jats:italic>, and <jats:italic>mmp9<\/jats:italic> emerging as key regulatory hubs. Widely targeted metabolomics revealed 891 bioactive compounds, including flavonoids and phenylpropanoids, with network pharmacology predicting multi-target interactions involving inflammatory and oxidative stress pathways. Molecular docking confirmed binding affinities between protocatechuic acid, L-pyroglutamic acid, and Serpine1\u2019s active site, indicating direct interference with inflammation modulation. Collectively, these results establish NEWE as a polypharmacological agent that disrupts inflammation-oxidative stress crosstalk primarily through Serpine1-mediated pathways, offering a molecular foundation for plant-derived interventions against chronic inflammatory diseases.<\/jats:p>","DOI":"10.3389\/fphar.2025.1612233","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T07:57:42Z","timestamp":1752220662000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Nymphaea \u201cEldorado\u201d flower extract targets serpine 1 to attenuate inflammatory and antioxidant crosstalk in zebrafish"],"prefix":"10.3389","volume":"16","author":[{"given":"Liyan","family":"Mao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuwei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiulan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqiong","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gongde","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beimi","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2061","DOI":"10.1016\/j.phytochem.2008.04.009","article-title":"Antioxidant constituents of Nymphaea caerulea flowers","volume":"69","author":"Agnihotri","year":"2008","journal-title":"Phytochemistry"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1762","DOI":"10.4049\/jimmunol.2300526","article-title":"Cutting edge: serpine1 negatively regulates Th1 cell responses in experimental autoimmune encephalomyelitis","volume":"211","author":"Akbar","year":"2023","journal-title":"J. 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