{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:24:51Z","timestamp":1787066691276,"version":"build-2736575974"},"reference-count":25,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>Dalea<\/jats:italic>\n                      genus [Fabaceae] is rich in bioactive flavonoids and contains underexplored species, such as Dalea pazensis Rusby, with potential for antifungal and dermatological applications.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      Considering the limited knowledge available on\n                      <jats:italic>D. pazensis,<\/jats:italic>\n                      this study aims to expand the current understanding of its chemical and biological potential.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>\n                      Sequential extraction of\n                      <jats:italic>D. pazensis<\/jats:italic>\n                      roots was performed using solvents of increasing polarity. Additionally, essential oil (EO) was obtained from the aerial parts. Extracts and EO were chemically characterized by ultra-performance liquid chromatography\u2013tandem mass spectrometry (UPLC-MS\/MS) and gas chromatography\/mass spectrometry (GC-MS), respectively. Antifungal activity was evaluated against azole-sensitive and azole-resistant\n                      <jats:italic>Candida albicans<\/jats:italic>\n                      strains, while tyrosinase inhibition was assessed in the different extracts using an\n                      <jats:italic>in vitro<\/jats:italic>\n                      enzymatic assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and discussion<\/jats:title>\n                    <jats:p>\n                      The chloroform extract (CDp) exhibited the most potent antifungal activity (minimum inhibitory concentration, MIC = 125\u00a0\u03bcg\/mL), a relevant value considering that the reference drug fluconazole shows an MIC of 32\u00a0\u03bcg\/mL in this resistant strain, underscoring the extract\u2019s significant activity despite azole resistance. CDp showed significant tyrosinase inhibition (half-maximal inhibitory concentration, IC\n                      <jats:sub>50<\/jats:sub>\n                      = 1.27\u00a0\u03bcg\/mL). UPLC-MS\/MS analysis identified (2\n                      <jats:italic>S<\/jats:italic>\n                      )-5,7,2\u2032,4\u2032-tetrahydroxy-5\u2032-(1\u2034,1\u2034-dimethylallyl)-8-prenylflavanone (compound 1) as the major constituent, previously linked to antifungal activity and efflux pump inhibition. EO analysis revealed \u03b2-caryophyllene (41.1%) as the main component, suggesting a distinct chemotype within the species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This is the first chemical report of the EO and the deepening of prenylflavonoid content in different extracts of\n                      <jats:italic>D. pazensis<\/jats:italic>\n                      , highlighting the pharmacological relevance of its dual antifungal and antityrosinase profile, a combination of interest for dermatological formulations targeting fungal infections, hyperpigmentation, or post-infectious dyschromias. The findings underscore this species as a promising source of prenylated flavanones with dual antifungal and anti-tyrosinase activity, as well as bioactive volatiles with antifungal activity. The results support its use in developing natural antifungal therapies, particularly against MDR pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2025.1717359","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T06:38:27Z","timestamp":1767595107000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Phytochemical characterization and pharmacological evaluation of aerial and root parts of Dalea pazensis Rusby [Fabaceae]"],"prefix":"10.3389","volume":"16","author":[{"given":"Mariana Andrea","family":"Peralta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Einy Nallybe","family":"Bedoya Aguirre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melisa Fabiana","family":"Negro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Echeverr\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Daniela","family":"Santi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Gabriela","family":"Ortega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"043101","DOI":"10.1063\/1.3653552","article-title":"Retention indices for frequently reported compounds of plant essential oils","volume":"40","author":"Babushok","year":"2011","journal-title":"J. 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