{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:07:54Z","timestamp":1791994074204,"version":"4.4.0"},"reference-count":57,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>\n                    Red grape pomace, a major by-product of the winemaking industry, represents a sustainable source of bioactive polyphenols with high nutraceutical and cosmetic potential. In this study, a standardized grape pomace extract (GPE) was developed from quality-controlled dried pomace, compliant with European Pharmacopoeia requirements for heavy metals, microbiological purity, and residual moisture. Phytochemical profiling by LC-DAD-ESI-MS\/MS revealed a complex composition dominated by anthocyanins\u2014primarily malvidin-3-\n                    <jats:italic>O<\/jats:italic>\n                    -glucoside\u2014alongside lignans, stilbenes, and flavonols. The extract showed a high total phenolic content (2.64\u00a0g GAE\/100\u00a0g) and exhibited strong, concentration-dependent antioxidant activity, with the highest efficacy in FRAP and ORAC assays, and significant anti-inflammatory effects in protein denaturation and protease inhibition tests. GPE demonstrated excellent photochemical stability and was incorporated into a lipid-based cosmetic formulation (SS + GPE) to assess its safety and photoprotective efficacy in a reconstructed human epidermis (RHE) model exposed to UVA (26\u00a0J\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) and UVB (1.5\u00a0J\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) radiation. Both GPE and SS + GPE treatments markedly reduced ROS, NO, and IL-1\u03b1 production (up to 60%\u201365% reduction vs. irradiated control) while maintaining cell viability above 90%. Histological analysis further confirmed the structural protection of the epidermis, showing fewer dyskeratotic cells, restored intercellular cohesion, and normalization of keratinization. Overall, these results demonstrate that GPE is a safe, photostable, and multifunctional bioactive extract capable of counteracting UV-induced oxidative and inflammatory damage. This study provides a comprehensive and sustainable strategy for the upcycling of red grape pomace into high value dermocosmetic formulations with proven broad-spectrum photoprotective efficacy.\n                  <\/jats:p>","DOI":"10.3389\/fphar.2025.1744377","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T07:43:34Z","timestamp":1767599014000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Harnessing red grape pomace extract for sustainable broad-spectrum photoprotection in a reconstructed human epidermis model"],"prefix":"10.3389","volume":"16","author":[{"given":"Antonella","family":"Smeriglio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Mangano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariarosaria","family":"Ingegneri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annarita","family":"La Neve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Brenna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Mastracci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Merlino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giancarlo","family":"Tonon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Bosco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Trombetta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"580","DOI":"10.3390\/foods13040580","article-title":"Grape pomace-advances in its bioactivity, health benefits, and food applications","volume":"13","author":"Almanza-Oliveros","year":"2024","journal-title":"Foods"},{"key":"B2","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.jclepro.2014.02.044","article-title":"Environmental impacts of consumption of Australian red wine in the UK","volume":"72","author":"Amienyo","year":"2014","journal-title":"J. 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