{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T20:06:17Z","timestamp":1780085177750,"version":"3.54.0"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021310","name":"Nigerian Institute of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021310","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Peptic ulcer disease (PUD) remains a global health burden, with current therapies limited by recurrence, adverse effects, and high costs.\n                      <jats:italic>Moringa oleifera<\/jats:italic>\n                      , a medicinal plant traditionally used for gastrointestinal disorders, has shown anti-ulcer potential in several experimental models. However, no systematic synthesis has consolidated the available preclinical evidence. This review evaluated the therapeutic efficacy of\n                      <jats:italic>M. oleifera<\/jats:italic>\n                      in PUD using evidence from preclinical (rodent) studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      We searched PubMed, CINAHL, Scopus, Web of Science, and the Cochrane Library from inception (without time filter) to August 2025. Eligible studies were randomized controlled animal experiments assessing\n                      <jats:italic>M. oleifera<\/jats:italic>\n                      extracts in ulcer-induced models. Data on ulcer index were extracted, and a random-effects meta-analysis was performed using Hedge\u2019s g to calculate standard mean difference (SMD). Heterogeneity was assessed with I\n                      <jats:sup>2<\/jats:sup>\n                      , and publication bias with Egger\u2019s regression and trim-and-fill methods.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Eleven (11) studies involving 268 animals met the inclusion criteria, with SYRCLE quality scores ranging from 8 to 9. Pooled analysis showed that\n                      <jats:italic>M. oleifera<\/jats:italic>\n                      significantly reduced ulcer index compared with controls (SMD = \u22121.42; 95% CI: \u22122.01 to \u22120.83; p &amp;lt; 0.01). Subgroup analyses revealed variability by dose and comparator. High-dose comparisons with standard drugs showed no significant difference (SMD = \u22120.03; 95% CI: \u22120.27 to 0.66; p = 0.92; I\n                      <jats:sup>2<\/jats:sup>\n                      = 69%), while comparisons with basal controls also lacked significance (SMD = 0.99; 95% CI: 0.24\u20132.22; p = 0.11). Sensitivity analyses resolved heterogeneity (I\n                      <jats:sup>2<\/jats:sup>\n                      = 0%) and publication bias, without altering overall outcomes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Moringa oleifera<\/jats:italic>\n                      demonstrates consistent gastroprotective and ulcer-healing effects in preclinical studies, though not superior to standard therapies. Methodological heterogeneity and the absence of clinical trials highlight the need for standardized experimental protocols and translational research to establish its role as a potential adjunctive therapy for PUD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Systematic Review Registration<\/jats:title>\n                    <jats:p>Identifier CRD420251080346.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2026.1689789","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T06:38:14Z","timestamp":1773988694000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Preclinical evidence of the therapeutic effect of Moringa oleifera in peptic ulcer disease: a systematic review and meta-analysis"],"prefix":"10.3389","volume":"17","author":[{"given":"Saheed Olatunbosun","family":"Akiode","sequence":"first","affiliation":[{"name":"Biotechnology Advanced Research Centre, Sheda Science and Technology Complex (SHESTCO)","place":["Garki, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeoti Gbemisola","family":"Adeniran","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Medical Sciences","place":["Ondo City, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oyedayo Phillips","family":"Akano","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Basic Medical Sciences, Olabisi Onabanjo University","place":["Ago Iwoye, Ogun, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayokunmi Stephen","family":"Olusa","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, Obafemi Awolowo University","place":["Ile-Ife, Osun State, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oghale Oghenefego","family":"Ovuakporie-Uvo","sequence":"additional","affiliation":[{"name":"Department of Science Laboratory Technology\/Centre for Herbal Medicine and Drug Discovery, University of Medical Sciences","place":["Ondo City, Ondo State, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluchukwu Perpetual","family":"Okeke","sequence":"additional","affiliation":[{"name":"Nigerian Institute of Medical Research Foundation","place":["Yaba, Lagos State, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olunike Rebecca","family":"Abodunrin","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatics, Nanjing Medical University","place":["Nanjing, China"]},{"name":"Center for Reproduction and Population Studies, Clinical Sciences Department, Nigerian Institute of Medical Research","place":["Yaba, Lagos State, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Folahanmi Tomiwa","family":"Akinsolu","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Population Studies, Clinical Sciences Department, Nigerian Institute of Medical Research","place":["Yaba, Lagos State, Nigeria"]},{"name":"Department of Research, Capacity Building and Implementation, Alabastron Initiative","place":["Lagos, Nigeria"]},{"name":"Department of Public Health, Faculty of Basic Medical and Health Sciences, Lead City University","place":["Ibadan, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwaisu","family":"Iliyasu","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Drug Development, Kaduna State University","place":["Kaduna, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olajide Odunayo","family":"Sobande","sequence":"additional","affiliation":[{"name":"Nigerian Institute of Medical Research Foundation","place":["Yaba, Lagos State, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamoru Abdulazeez","family":"Adeniyi","sequence":"additional","affiliation":[{"name":"Department of Animal and Environmental Biology, Federal University Dutse","place":["Dutse, Jigawa State, Nigeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,20]]},"reference":[{"key":"B1","first-page":"189","article-title":"Proximate analysis and anti-ulcer activity of methanolic extract of Moringa oleifera","volume":"20","author":"Abdu","year":"2021","journal-title":"Afr. 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