{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T21:16:19Z","timestamp":1773090979898,"version":"3.50.1"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>\n                    In this study, a liquid chromatography-tandem mass spectrometry (LC-MS\/MS) method was developed and validated for the quantitative determination of amantadine in human plasma, with the incorporation of an internal standard to improve analytical accuracy. Plasma samples collected from volunteers were processed using acetonitrile-methanol (3:1, v\/v) as the extraction solvent, followed by protein precipitation and purification via the QuEChERS (Quick, Easy, Cheap, Efficacious, Rugged, and Safe) method. Analysis was performed using LC-MS\/MS under multiple reaction monitoring mode, with a total run time of 8\u00a0min. Quantification was carried out using the internal standard method. After a single oral administration of 200\u00a0mg amantadine hydrochloride, plasma concentrations were measured at various time points. Pharmacokinetic parameters were derived by fitting the data to a pharmacokinetic model using specialized software. The results demonstrated good linearity over the range of 0.5\u201320\u00a0ng\/mL, with a correlation coefficient (R\n                    <jats:sup>2<\/jats:sup>\n                    ) of 0.9978. The extraction recovery ranged from 94.5% to 110.1%, and both intra-day and inter-day relative standard deviations (RSD) were below 10%. The limit of detection (LOD) and limit of quantification (LOQ) were 0.15\u00a0ng\/mL and 0.5\u00a0ng\/mL, respectively. The absorption and elimination processes of amantadine in plasma followed first-order kinetics, with R\n                    <jats:sup>2<\/jats:sup>\n                    &amp;gt; 0.9. Notably, gender-specific differences were observed in the time to maximum concentration (T\n                    <jats:sub>max<\/jats:sub>\n                    ) and maximum concentration (C\n                    <jats:sub>max<\/jats:sub>\n                    ): females achieved a C\n                    <jats:sub>max<\/jats:sub>\n                    of 670.23\u00a0ng\/mL at 4\u00a0h, whereas males reached a C\n                    <jats:sub>max<\/jats:sub>\n                    of 650.87\u00a0ng\/mL at 8\u00a0h. This LC-MS\/MS method is simple, rapid, and accurate, rendering it suitable for pharmacokinetic studies of amantadine in humans. Additionally, the established kinetic model provides valuable references for clinical medication guidance.\n                  <\/jats:p>","DOI":"10.3389\/fphar.2026.1731595","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T09:06:31Z","timestamp":1770023191000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of an LC-MS\/MS method for amantadine detection in human plasma using ZIF-8 as adsorbent and pharmacokinetic investigation"],"prefix":"10.3389","volume":"17","author":[{"given":"Zhengang","family":"Wang","sequence":"first","affiliation":[{"name":"Affiliated Hospital of Shandong Second Medical University","place":["Weifang, Shandong, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanmei","family":"Han","sequence":"additional","affiliation":[{"name":"Jinan International Travel Healthcare Center","place":["Jinan, Shandong, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxia","family":"Li","sequence":"additional","affiliation":[{"name":"Weifang Customs","place":["Weifang, Shandong, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Weifang Customs","place":["Weifang, Shandong, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuiying","family":"Ding","sequence":"additional","affiliation":[{"name":"Weifang Customs","place":["Weifang, Shandong, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"111724.1","DOI":"10.1016\/j.microc.2024.111724","article-title":"Common and novel methods for the identifica-tion of bisphenol A in tea samples: a systematic review study","volume":"207","author":"Akbari","year":"2024","journal-title":"J.Microchem J."},{"key":"B2","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.jpha.2017.10.003","article-title":"Sensitive and rapid determination of amantadine without derivatization in human plasma by LC-MS\/MS for a bioequivalence study","volume":"8","author":"Bhadoriya","year":"2018","journal-title":"J.Pharm Anal."},{"key":"B3","doi-asserted-by":"publisher","first-page":"105915","DOI":"10.1016\/j.jfca.2023.105915","article-title":"MIL-53\/UiO-66\/PAN beads as a novel adsorbent for simultaneous extraction of trace pesticides in wheat, wheat flour and field soil followed by GC-MS analysis","volume":"126","author":"Cai","year":"2024","journal-title":"J.Food Compos Anal."},{"key":"B4","doi-asserted-by":"publisher","first-page":"103032","DOI":"10.1016\/j.ceca.2025.103032","article-title":"Stable expression of SARS-CoV-2 envelope viroporin promotes intracellular calcium depletion in human cells: relevance for endoplasmic reticulum stress, cell proliferation, pluripotency and lineage differentiation","volume":"128","author":"Cesare","year":"2025","journal-title":"J.Cell Calcium"},{"key":"B5","doi-asserted-by":"publisher","first-page":"111572","DOI":"10.1016\/j.jviromet.2025.115172","article-title":"D-cycloserine, a potential candidate for reducing hepatitis B virus cccDNA in vitro","volume":"336","author":"Choi","year":"2025","journal-title":"J.Virological Methods"},{"key":"B6","doi-asserted-by":"publisher","first-page":"233","DOI":"10.3390\/metabo15040233","article-title":"Recent advances in pretreatment methods and detection techniques for veterinary drug residues in animal-derived foods","volume":"15","author":"Dai","year":"2025","journal-title":"J. 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