{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:36:57Z","timestamp":1776836217678,"version":"3.51.2"},"reference-count":108,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>\n                    Excessive exposure to ultraviolet (UV) radiation induces oxidative stress, DNA damage, and extracellular matrix (ECM) disorganization, contributing to skin aging and disease. To enhance photoprotection strategies, antioxidant molecules have been explored as complementary agents to conventional UV filters. Here, we investigated the photoprotective potential of\n                    <jats:italic>N\u2032,N\u2034,N\u2034'<\/jats:italic>\n                    '-((1\n                    <jats:italic>E<\/jats:italic>\n                    ,1\u2032\n                    <jats:italic>E,<\/jats:italic>\n                    1\u2033\n                    <jats:italic>E<\/jats:italic>\n                    )-(((1,3,5-triazine-2,4,6-triyl)tris(oxy))tris(benzene-4,1-diyl))tris(methanylylidene))tris(2-hydroxybenzohydrazide) (Trz-HBH\n                    <jats:sub>3<\/jats:sub>\n                    ), a novel resveratrol analogue obtained through molecular triplication. The compound was evaluated for UV absorption, photostability, phototoxicity in 3T3 fibroblasts, and protective effects against UVB-induced damage in HaCaT keratinocytes. To improve physiological relevance, three-dimensional (3D) human skin models were developed using fibroblasts and keratinocytes, with or without human umbilical vein endothelial cells (HUVEC), and assembled manually or by extrusion-based bioprinting. Models were characterized by Calcein-AM staining and (immuno)histochemical analysis. To assess oxidative stress responses, tissues were exposed to UVA radiation from two sources (UVA lamp and sun simulator), and intracellular reactive oxygen species (ROS) levels were quantified. While Trz-HBH\n                    <jats:sub>3<\/jats:sub>\n                    showed no cytotoxic or phototoxic effects, it exhibited broad UV absorption (in the UVB, UVA II and part of UVA I), high photostability (remaining absorbance values of 93.8% \u00b1 2.8% in the UVA region and 86.0% \u00b1 8.0% in the UVB region), and protective effects against UVB-induced damage, maintaining cell viability close to 100%. Notably, Trz-HBH\n                    <jats:sub>3<\/jats:sub>\n                    effectively reduced UVA-induced ROS by 63.5% and 69.5%, in both reconstructed and endothelialized bioprinted human skin models, respectively. These findings support the antioxidant and photoprotective properties of Trz-HBH\n                    <jats:sub>3<\/jats:sub>\n                    and reinforce the applicability of bioprinted skin as an advanced\n                    <jats:italic>in vitro<\/jats:italic>\n                    platform to investigate UV-induced skin responses.\n                  <\/jats:p>","DOI":"10.3389\/fphar.2026.1756483","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T04:37:33Z","timestamp":1776832653000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Photoprotection of a novel resveratrol analogue: insights from 2D, 3D, and endothelialized bioprinted human skin models"],"prefix":"10.3389","volume":"17","author":[{"given":"Ana J\u00falia","family":"Pasuch Gluzezak","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo","place":["Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Essayan","sequence":"additional","affiliation":[{"name":"3d.FAB, Universit\u00e9 Lyon 1, CNRS, INSA, CPE-Lyon, ICBMS","place":["Villeurbanne, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celine","family":"Thomann","sequence":"additional","affiliation":[{"name":"3d.FAB, Universit\u00e9 Lyon 1, CNRS, INSA, CPE-Lyon, ICBMS","place":["Villeurbanne, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean Leandro","family":"dos Santos","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences of UNESP","place":["Araraquara, S\u00e3o Paulo, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe A.","family":"Marquette","sequence":"additional","affiliation":[{"name":"3d.FAB, Universit\u00e9 Lyon 1, CNRS, INSA, CPE-Lyon, ICBMS","place":["Villeurbanne, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena Rigo","family":"Gaspar","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo","place":["Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.jphotobiol.2014.07.004","article-title":"Photodegradation of avobenzone: stabilization effect of antioxidants","volume":"140","author":"Afonso","year":"2014","journal-title":"J. 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