{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T06:51:36Z","timestamp":1781679096917,"version":"3.54.5"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"\n Objective<\/jats:title>\n \n This study aimed to investigate the protective effects of a novel composite comprising\n Lactobacillus<\/jats:italic>\n paracasei and\n Puerariae Radix<\/jats:italic>\n extract (Lac_PRE) against hydrogen peroxide (H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n )-induced multi-organ oxidative damage in a zebrafish model.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n An acute toxicity test was conducted to determine the optimal subacute H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n exposure concentration in wild-type AB strain zebrafish. Subsequently, zebrafish were divided into four groups: control, H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n exposure (2.0\u00a0mmol\/L), H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n exposure with Lac_PRE treatment, and Lac_PRE control. After 14 days, we assessed hepatic and cardiac function markers, inflammatory gene expression (cyclooxygenase, cox; tumor necrosis factor-alpha, tnf-\u03b1; interleukin-6, il-6), oxidative stress indicators (malondialdehyde, MDA), and the intestinal Firmicutes\/Bacteroidetes (F\/B) ratio.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n exposure induced significant oxidative and inflammatory damage in zebrafish, Kruskal-Wallis tests revealed significant overall differences among groups for hepatic ALT, hepatic MDA, cardiac cTnI, cardiac MDA, cardiac COX, cardiac TNF-\u03b1, cardiac IL-6, and the intestinal F\/B ratio (all p &lt; 0.05). Two a priori planned comparisons were tested using Dunn\u2019s post hoc test with Bonferroni \u00d72 correction. H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n exposure significantly elevated cardiac cTnI (adjusted p = 0.002), cardiac MDA (adjusted p = 0.015), cardiac COX (adjusted p = 0.029), TNF-\u03b1 (adjusted p = 0.023), IL-6 (adjusted p = 0.005), and significantly disrupted the F\/B ratio (adjusted p = 0.012) compared to control. The Lac_PRE composite significantly reduced hepatic ALT (adjusted p &lt; 0.001) and cardiac COX expression (adjusted p = 0.023). For the remaining indicators, the H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n _Lac_PRE group showed consistent numerical improvements, but these differences did not reach statistical significance after Bonferroni correction.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The L. paracasei and\n Puerariae Radix<\/jats:italic>\n extract composite effectively alleviates H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n -induced oxidative inflammatory damage in zebrafish by enhancing antioxidant defenses, suppressing inflammation, and modulating gut microbiota. These preliminary findings suggest that this probiotic-botanical combination may exert synergistic protective effects against oxidative damage. However, given the methodological limitations including small effective sample sizes, these results should be interpreted as exploratory and hypothesis-generating. Further validation in studies with larger sample sizes and individual-level analyses is warranted before translational applications can be considered.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fphar.2026.1787487","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T05:52:12Z","timestamp":1781675532000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Ameliorative effects of a Lactobacillus paracasei and Puerariae Radix extract complex on hydrogen peroxide-induced oxidative damage in zebrafish"],"prefix":"10.3389","volume":"17","author":[{"given":"Suyu","family":"Chen","sequence":"first","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]},{"name":"School of Public Health, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Tang","sequence":"additional","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]},{"name":"School of Public Health, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Junhong","sequence":"additional","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]},{"name":"School of Public Health, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Bosi","sequence":"additional","affiliation":[{"name":"School of Public Health, Huazhong University of Science and Technology","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianpeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dafeng","family":"Lin","sequence":"additional","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangli","family":"Yang","sequence":"additional","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peimao","family":"Li","sequence":"additional","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naixing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2341","DOI":"10.3390\/microorganisms12112341","article-title":"Intestinal insights: the gut microbiome's role in atherosclerotic disease: a narrative review","volume":"12","author":"Alexandrescu","year":"2024","journal-title":"Microorganisms"},{"key":"B2","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1186\/s40360-018-0216-3","article-title":"Absolute oral bioavailability and disposition kinetics of puerarin in female rats","volume":"19","author":"Anukunwithaya","year":"2018","journal-title":"BMC Pharmacol. 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