{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T05:53:57Z","timestamp":1776750837791,"version":"3.51.2"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006321","name":"National Institutes of Natural Sciences","doi-asserted-by":"publisher","award":["82341233"],"award-info":[{"award-number":["82341233"]}],"id":[{"id":"10.13039\/501100006321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Ulcerative colitis (UC) is being increasingly connected to the pathogen\n                      <jats:italic>Veillonella parvula<\/jats:italic>\n                      (V. parvula). Modified Gegen Qinlian Decoction (MGQD), a traditional Chinese medicine therapy, is used to treat UC. However, the underlying mechanism by which V. parvula exacerbates UC and whether MGQD can alleviate it is unknown.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      MGQD therapy was tested on pathogen-free and pseudo-germ-free mice infected with V. parvula. Colitis severity was determined by disease activity index, colon length, and histology. Transmission electron microscopy, Western blot, immunofluorescence, and immunohistochemistry were used to assess the integrity of the intestinal mucosal barrier.\n                      <jats:italic>In vitro<\/jats:italic>\n                      , mouse colonic organoids were utilized to examine the effects of baicalin and puerarin on inflammation-induced downregulation of Muc2 and ZO-1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MGQD therapy dramatically reduced the severity of colitis in both mouse models. MGQD restored the integrity of the mucus and mechanical barriers. Furthermore, baicalin and puerarin reduced inflammation-induced downregulation of Muc2 and ZO-1 in mouse colonic organoids.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings demonstrate that MGQD alleviates intestinal barrier dysfunction caused by V. parvula, providing novel mechanistic insight for the management of chronic colitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2026.1803816","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T05:34:46Z","timestamp":1776749686000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Modified gegen qinlian decoction ameliorates Veillonella parvula-exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function"],"prefix":"10.3389","volume":"17","author":[{"given":"Meiling","family":"She","sequence":"first","affiliation":[{"name":"Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences","place":["Beijing, China"]},{"name":"China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyi","family":"Dai","sequence":"additional","affiliation":[{"name":"Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences","place":["Beijing, China"]},{"name":"China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xintong","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences","place":["Beijing, China"]},{"name":"China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuo","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shanghai Jiao Tong University","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaobo","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shanghai Jiao Tong University","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xudong","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"951558","DOI":"10.3389\/fphar.2022.951558","article-title":"Huangkui lianchang decoction attenuates experimental colitis by inhibiting the NF-\u03baB pathway and autophagy","volume":"13","author":"Cheng","year":"2022","journal-title":"Front. Pharmacol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1445838","DOI":"10.3389\/fimmu.2024.1445838","article-title":"Modified Gegen Qinlian Decoction modulated the gut microbiome and bile acid metabolism and restored the function of goblet cells in a mouse model of ulcerative colitis","volume":"15","author":"Huang","year":"2024","journal-title":"Front. Immunol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1136\/gutjnl-2012-303207","article-title":"Bacteria penetrate the normally impenetrable inner colon mucus layer in both murine colitis models and patients with ulcerative colitis","volume":"63","author":"Johansson","year":"2014","journal-title":"Gut"},{"key":"B4","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1136\/gut.52.5.713","article-title":"Vanilloid receptor-1 containing primary sensory neurones mediate dextran sulphate sodium induced colitis in rats","volume":"52","author":"Kihara","year":"2003","journal-title":"Gut"},{"key":"B5","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1093\/ecco-jcc\/jjac177","article-title":"Increasing incidence of inflammatory bowel disease, with greatest change among the elderly: a nationwide study in Finland, 2000-2020","volume":"17","author":"Kontola","year":"2023","journal-title":"J. Crohns Colitis"},{"key":"B6","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1016\/S0140-6736(23)00966-2","article-title":"Ulcerative colitis","volume":"402","author":"Le Berre","year":"2023","journal-title":"Lancet"},{"key":"B7","doi-asserted-by":"publisher","first-page":"e2400206","DOI":"10.1002\/advs.202400206","article-title":"Gingerenone A attenuates ulcerative colitis via targeting IL-17RA to inhibit inflammation and restore intestinal barrier function","volume":"11","author":"Liang","year":"2024","journal-title":"Adv. Sci. (Weinh)"},{"key":"B8","doi-asserted-by":"publisher","first-page":"115084","DOI":"10.1016\/j.jep.2022.115084","article-title":"Potential activity of traditional Chinese medicine against ulcerative colitis: a review","volume":"289","author":"Liu","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"155329","DOI":"10.1016\/j.phymed.2023.155329","article-title":"Study on the mechanism of modified Gegen Qinlian decoction in regulating the intestinal flora-bile acid-TGR5 axis for the treatment of type 2 diabetes mellitus based on macro genome sequencing and targeted metabonomics integration","volume":"132","author":"Liu","year":"2024","journal-title":"Phytomedicine"},{"key":"B10","doi-asserted-by":"publisher","first-page":"154660","DOI":"10.1016\/j.phymed.2023.154660","article-title":"Modified Gegen Qinlian decoction ameliorates DSS-induced chronic colitis in mice by restoring the intestinal mucus barrier and inhibiting the activation of \u03b3\u03b4T17 cells","volume":"111","author":"Ma","year":"2023","journal-title":"Phytomedicine"},{"key":"B11","doi-asserted-by":"publisher","first-page":"e20211938","DOI":"10.1084\/jem.20211938","article-title":"Intelectin-1 binds and alters the localization of the mucus barrier-modifying bacterium Akkermansia muciniphila","volume":"220","author":"Matute","year":"2023","journal-title":"J. Exp. Med."},{"key":"B12","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1038\/s41575-020-00399-w","article-title":"The intestinal barrier, an arbitrator turned provocateur in IBD","volume":"18","author":"Mehandru","year":"2021","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"2769","DOI":"10.1016\/S0140-6736(17)32448-0","article-title":"Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: a systematic review of population-based studies","volume":"390","author":"Ng","year":"2017","journal-title":"Lancet"},{"key":"B14","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1016\/j.jhep.2021.09.010","article-title":"Rifaximin-\u03b1 reduces gut-derived inflammation and mucin degradation in cirrhosis and encephalopathy: RIFSYS randomised controlled trial","volume":"76","author":"Patel","year":"2022","journal-title":"J. Hepatol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"112846","DOI":"10.1016\/j.intimp.2024.112846","article-title":"Morin alleviates DSS-induced ulcerative colitis in mice via inhibition of inflammation and modulation of intestinal microbiota","volume":"140","author":"Qiu","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1673","DOI":"10.1038\/s41564-022-01224-7","article-title":"Inflammation-associated nitrate facilitates ectopic colonization of oral bacterium Veillonella parvula in the intestine","volume":"7","author":"Rojas-Tapias","year":"2022","journal-title":"Nat. Microbiol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"948","DOI":"10.1053\/j.gastro.2017.11.276","article-title":"Pyrin inflammasome regulates tight junction integrity to restrict colitis and tumorigenesis","volume":"154","author":"Sharma","year":"2018","journal-title":"Gastroenterology"},{"key":"B18","doi-asserted-by":"publisher","first-page":"109940","DOI":"10.1016\/j.jnutbio.2025.109940","article-title":"Ursolic acid attenuates sarcopenia through IL-17a-related gut-muscle axis in senile diabetic mice and myotube model","volume":"143","author":"She","year":"2025","journal-title":"J. Nutr. Biochem."},{"key":"B19","doi-asserted-by":"publisher","first-page":"2142","DOI":"10.1136\/gutjnl-2018-317571","article-title":"Structural weakening of the colonic mucus barrier is an early event in ulcerative colitis pathogenesis","volume":"68","author":"van der Post","year":"2019","journal-title":"Gut"},{"key":"B20","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1136\/gutjnl-2012-304066","article-title":"Functional bowel symptoms in quiescent inflammatory bowel diseases: role of epithelial barrier disruption and low-grade inflammation","volume":"63","author":"Vivinus-N\u00e9bot","year":"2014","journal-title":"Gut"},{"key":"B21","doi-asserted-by":"publisher","first-page":"756978","DOI":"10.3389\/fphar.2021.756978","article-title":"Modified Gegen Qinlian Decoction Regulates Treg\/Th17 Balance to Ameliorate DSS-Induced Acute Experimental Colitis in Mice by Altering the Gut Microbiota","volume":"12","author":"Wang","year":"2021","journal-title":"Front. Pharmacol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"116538","DOI":"10.1016\/j.jep.2023.116538","article-title":"Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro","volume":"313","author":"Wang","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1016\/j.tips.2024.08.003","article-title":"Ulcerative colitis: clinical biomarkers, therapeutic targets, and emerging treatments","volume":"45","author":"Wangchuk","year":"2024","journal-title":"Trends Pharmacol. Sci."},{"key":"B24","doi-asserted-by":"publisher","first-page":"114103","DOI":"10.1016\/j.jep.2021.114103","article-title":"Zuojin Pill ameliorates inflammation in indomethacin-induced gastric injury via inhibition of MAPK pathway","volume":"275","author":"Wei","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"155730","DOI":"10.1016\/j.phymed.2024.155730","article-title":"Narirutin mitigates dextrose sodium sulfate-induced colitis in mice by modulating intestinal flora","volume":"130","author":"Xu","year":"2024","journal-title":"Phytomedicine"},{"key":"B26","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1038\/s41420-022-01015-3","article-title":"Overabundance of Veillonella parvula promotes intestinal inflammation by activating macrophages via LPS-TLR4 pathway","volume":"8","author":"Zhan","year":"2022","journal-title":"Cell Death Discov."},{"key":"B27","doi-asserted-by":"publisher","first-page":"2247","DOI":"10.3389\/fmicb.2018.02247","article-title":"The gut microbiota in the pathogenesis and therapeutics of inflammatory bowel disease","volume":"9","author":"Zuo","year":"2018","journal-title":"Front. Microbiol."}],"container-title":["Frontiers in Pharmacology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2026.1803816\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T05:34:50Z","timestamp":1776749690000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2026.1803816\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,21]]},"references-count":27,"alternative-id":["10.3389\/fphar.2026.1803816"],"URL":"https:\/\/doi.org\/10.3389\/fphar.2026.1803816","relation":{},"ISSN":["1663-9812"],"issn-type":[{"value":"1663-9812","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,21]]},"article-number":"1803816"}}