{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T06:13:55Z","timestamp":1779257635431,"version":"3.51.4"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study investigated the protective effects of a commercial total flavonoid preparation derived from\n                      <jats:italic>Puerariae Lobatae Radix<\/jats:italic>\n                      on exhaustive exercise\u2013induced liver injury in mice, with a focus on oxidative stress, inflammatory responses, and NF-\u03baB\u2013related signaling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A mouse model of exhaustive exercise\u2013induced liver injury was established by exhaustive swimming. Mice were assigned to a normal control group, an exercise model group, a silymarin-treated group, and low-, medium-, and high-dose total flavonoid preparation groups. Liver function, histopathological alterations, oxidative stress\u2013related parameters, inflammatory mediators, and NF-\u03baB\u2013related signaling proteins were evaluated after 4 weeks of treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Exhaustive exercise induced marked liver injury, as evidenced by elevated serum ALT and AST levels, histopathological damage, aggravated oxidative stress, and increased inflammatory responses. In the exercise model group, serum GSH decreased from 155.77 \u00b1 9.32 to 71.64 \u00b1 4.20\u00a0\u03bcmol\/L, hepatic MDA increased from 3.80 \u00b1 1.50 to 8.34 \u00b1 1.58\u00a0nmol\/mg protein, hepatic GSH decreased from 1.43 \u00b1 0.05 to 1.00 \u00b1 0.07\u00a0nmol\/mg protein, and hepatic SOD decreased from 0.92 \u00b1 0.56 to 0.33 \u00b1 0.21\u00a0U\/mg protein. Treatment with the tested total flavonoid preparation attenuated these alterations, with the medium-dose group showing the most consistent effects, including restoration of serum GSH to 135.84 \u00b1 10.49\u00a0\u03bcmol\/L, reduction of hepatic MDA to 4.07 \u00b1 0.68\u00a0nmol\/mg protein, increase of hepatic GSH to 1.35 \u00b1 0.05\u00a0nmol\/mg protein, and increase of hepatic SOD to 0.86 \u00b1 0.07\u00a0U\/mg protein. The preparation also reduced inflammatory mediator expression and modulated NF-\u03baB\u2013related signaling, as reflected by decreased p-IKK\u03b1 and increased p-I\u03baB\u03b1 expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The tested total flavonoid preparation attenuated exhaustive exercise\u2013induced liver injury in mice. Its protective effects were associated with reduced oxidative stress, suppression of inflammatory responses, and modulation of NF-\u03baB\u2013related signaling, supporting its potential value in preventing exercise-related hepatic stress.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2026.1823011","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T05:46:43Z","timestamp":1779256003000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective effects of a commercial total flavonoid preparation from Puerariae Lobatae Radix on exhaustive exercise\u2013induced liver injury in mice"],"prefix":"10.3389","volume":"17","author":[{"given":"Zhenmao","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Medical Humanities, Anhui Medical University","place":["Hefei, Anhui, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Clinical College, Anhui Medical University","place":["Hefei, Anhui, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengkun","family":"Zhao","sequence":"additional","affiliation":[{"name":"The First Clinical College, Anhui Medical University","place":["Hefei, Anhui, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangchen","family":"Qiu","sequence":"additional","affiliation":[{"name":"The First Clinical College, Anhui Medical University","place":["Hefei, Anhui, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ding","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Medical Humanities, Anhui Medical University","place":["Hefei, Anhui, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"B1","first-page":"48","article-title":"Total flavonoids from puerariae lobatae radix alleviate myocardial injury in mice subjected to exhaustive exercise by downregulating the NF-\u03baB signaling pathway","volume":"39","author":"Chen","year":"2023"},{"key":"B2","volume-title":"Pharmacopoeia of the people\u2019s Republic of China","year":"2020"},{"key":"B3","doi-asserted-by":"publisher","first-page":"118468","DOI":"10.1016\/j.jep.2024.118468","article-title":"Pueraria Lobata antioxidant extract ameliorates non-alcoholic fatty liver by altering hepatic fat accumulation and oxidative stress","volume":"333","author":"Dong","year":"2024","journal-title":"J. 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