{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:52:12Z","timestamp":1791525132662,"version":"4.3.4"},"reference-count":90,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Estrogen deficiency following ovariectomy produces metabolic, skeletal, and uterine alterations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, we evaluated an authenticated and chemically characterized dichloromethane\u2013methanol (1:1, v\/v) root extract of\n                      <jats:italic>Dolomiaea costus<\/jats:italic>\n                      (Syn.\n                      <jats:italic>Saussurea costus<\/jats:italic>\n                      ) in ovariectomized female Wistar rats. Thirty rats were assigned to sham-operated, ovariectomized untreated, and ovariectomized extract-treated groups. Beginning 3\u00a0weeks after surgery, the treated group received the extract orally at 300\u00a0mg\/kg body weight\/day for 12 weeks. Metabolic and lipid variables, adipokines, antioxidant status, and bone-related biomarkers were determined. Uterine, femoral, hepatic, and adipose tissues were examined histologically, while estrogen receptor-\u03b1 (ER-\u03b1) and tumor necrosis factor-\u03b1 (TNF- \u03b1) immunoreactivity were evaluated. The extract was characterized using spectrophotometric assays, GC\u2013MS, and HPLC-DAD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ovariectomy was associated with increased body and adipose-tissue mass, dyslipidemia, impaired glucose homeostasis, altered adipokine concentrations, decreased antioxidant capacity, and structural alterations in the uterus, bone, liver, and adipose tissue. Compared with untreated ovariectomized rats, extract administration was associated with improvements in several biochemical and histological outcomes and with increased uterine ER-\u03b1 immunoreactivity. Chemical profiling indicated the presence of sesquiterpene lactones, fatty-acid derivatives, and phenolic metabolites.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Increased ER-\u03b1 immunoreactivity is consistent with an estrogen-responsive effect but does not establish direct estrogen-receptor agonism. These preclinical findings support further standardized pharmacological and toxicological evaluation of the extract; they do not establish efficacy or safety in menopausal women.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2026.1841164","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:35:17Z","timestamp":1791524117000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical characterization and effects of Dolomiaea costus root extract on ovariectomy-induced metabolic, skeletal, and uterine alterations in rats"],"prefix":"10.3389","volume":"17","author":[{"given":"Tahany Saleh","family":"Aldayel","sequence":"first","affiliation":[{"name":"Department of Health Sciences, Clinical Nutrition, College of Health and Rehabilitation Sciences, Princess Nourah bint Abdulrahman University","place":["Riyadh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany Salah","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Center of Scientific Foundation for Experimental Studies and Research","place":["Ismailia, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba M. A.","family":"Abdelrazek","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University","place":["Ismailia, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doaa H.","family":"Elsayed","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University","place":["Ismailia, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona A.","family":"Higo","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science, Al-Arish University","place":["North Sinai, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enas E.","family":"Eltamany","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University","place":["Ismailia, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba Nageh","family":"Gad EL-Hak","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Sciences, Suez Canal University","place":["Ismailia, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"5713606","DOI":"10.1155\/2019\/5713606","article-title":"Soy isoflavones ameliorate metabolic and immunological alterations of ovariectomy in female wistar rats: antioxidant and estrogen sparing potential","volume":"2019","author":"Abdelrazek","year":"2019","journal-title":"Oxidative Medicine Cellular Longevity"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2377","DOI":"10.1007\/s00198-013-2313-x","article-title":"NF-\u03baB signaling and bone resorption","volume":"24","author":"Abu-Amer","year":"2013","journal-title":"J. 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