{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:16:15Z","timestamp":1786115775405,"version":"build-2736575974"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>To evaluate the effect of Icariin (ICA) on AOM\/DSS-induced colorectal cancer (CRC) in mice as well as CRC cells, and to explore the underlying molecular mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The therapeutic efficacy of ICA against AOM\/DSS-induced CRC was evaluated by detecting tumor growth and pathological alterations. Biomarkers related to Wnt\/\u03b2-catenin and mitochondrial apoptosis were tested, and cell experiments were performed to validate the regulatory correlation between ICA and the Wnt\/\u03b2-catenin pathway in CRC cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ICA significantly reduced tumor number, size and ameliorated pathological damage in AOM\/DSS-induced mice by regulating the mitochondrial-mediated apoptosis of colon tissue. ICA\u2019s regulation of mitochondrial-mediated apoptosis was confirmed to involve the Wnt\/\u03b2-catenin signaling pathway.\n                      <jats:italic>In vitro<\/jats:italic>\n                      , ICA restrained proliferation, migration and invasion of SW1463 and HCT116 cells, decreased mitochondrial membrane potential and changed the expression of pathway-related genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study demonstrated that ICA promoted mitochondrial-mediated apoptosis by regulating the Wnt\/\u03b2-catenin signaling pathway, suggesting that ICA could serve as a potential treatment for CRC.<\/jats:p>\n                  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