{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:46:08Z","timestamp":1782794768833,"version":"3.54.5"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Encephalomyocarditis virus (EMCV), a highly significant member of the picornaviridae family, infects a broad range of mammalian hosts and causes severe pathological consequences like encephalitis, myocarditis, and neurologic diseases, etc. In our previous research, curcumol demonstrated a significant reduction in EMCV replication\n                      <jats:italic>in vitro<\/jats:italic>\n                      . However, its antiviral effect\n                      <jats:italic>in vivo<\/jats:italic>\n                      needs to be elucidated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, the mechanism of curcumol against EMCV replication was demonstrated\n                      <jats:italic>in vivo<\/jats:italic>\n                      by establishing an EMCV (100 TCID\n                      <jats:sub>50<\/jats:sub>\n                      \/20 g) infected mice model. At day 3 post EMCV infection, drug treatment groups were continuously treated with curcumol for 5 days.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The viral load and expression of interleukins such as\n                      <jats:italic>IL-1\u03b2, IL-6,<\/jats:italic>\n                      and\n                      <jats:italic>TNF-\u03b1<\/jats:italic>\n                      mRNA levels were significantly decreased in the heart and brain tissues at day 9 of EMCV infection (5 days of curcumol treatment), detected by qPCR. Moreover, Western blot analysis revealed that curcumol considerably elevated the protein levels of I\u03baB and P65, while significantly reducing the levels of P-I\u03baB, P-P65, NLRP3 inflammasome, caspase 1, and IL-1\u03b2 in cardiac and cerebral tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Conclusively, these results revealed that curcumol inhibits the NF-\u03baB\/NLRP3 inflammasome pathway and thus alleviates heart and brain damage induced by EMCV infection in Kunming mice. The present findings provide new insights into the therapeutic potential of curcumol in addressing EMCV-induced pathological conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2026.1865874","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:24:40Z","timestamp":1782793480000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Curcumol alleviates cardiac and cerebral injury caused by encephalomyocarditis virus (EMCV) in Kunming mice via suppressing NF-\u03baB\/NLRP3 inflammasome pathway"],"prefix":"10.3389","volume":"17","author":[{"given":"Panpan","family":"Sun","sequence":"first","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University","place":["Taigu, Shanxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangang","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Public Health, Changzhi Medical College","place":["Changzhi, Shanxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Haseeb","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University","place":["Taigu, Shanxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University","place":["Taigu, Shanxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Sun","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University","place":["Taigu, Shanxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaogui","family":"Sun","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University","place":["Taigu, Shanxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University","place":["Taigu, Shanxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoyu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Dentistry and Medical Sciences, Faculty of Science and Health, Charles Sturt University","place":["Orange, NSW, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongquan","family":"Li","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University","place":["Taigu, Shanxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1161\/CIRCRESAHA.120.315937","article-title":"Interleukin-1 and the inflammasome as therapeutic targets in cardiovascular disease","volume":"126","author":"Abbate","year":"2020","journal-title":"Circulation Research"},{"key":"B2","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1007\/s10741-018-9716-x","article-title":"Role of cytokines and inflammation in heart function during health and disease","volume":"23","author":"Bartekova","year":"2018","journal-title":"Heart Failure Reviews"},{"key":"B3","doi-asserted-by":"publisher","first-page":"2449","DOI":"10.1080\/15548627.2020.1831800","article-title":"Essential role of autophagy in restricting poliovirus infection revealed by identification of an ATG7 defect in a poliomyelitis patient","volume":"17","author":"Brinck Andersen","year":"2021","journal-title":"Autophagy"},{"key":"B4","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1186\/s12985-016-0653-9","article-title":"Encephalomyocarditis virus infection in Macaca sylvanus and hystrix cristata from an Italian rescue centre for wild and exotic animals","volume":"13","author":"Cardeti","year":"2016","journal-title":"Virology J."},{"key":"B5","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.ejps.2014.09.001","article-title":"Curcumol induces HSC-T6 cell death through suppression of Bcl-2: involvement of PI3K and NF-\u03baB pathways","volume":"65","author":"Chen","year":"2014","journal-title":"Eur. 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