{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:00:50Z","timestamp":1785477650221,"version":"3.56.0"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxidation (LPO), represents a promising therapeutic strategy.\n                      <jats:italic>Magnolia officinalis<\/jats:italic>\n                      , a traditional herb for resolving dampness and phlegm, is known to modulate cellular metabolism. While magnolol (MAG), a bioactive neolignan from\n                      <jats:italic>M. officinalis<\/jats:italic>\n                      , shows anti-TNBC activity, its role in inducing ferroptosis remains unexplored.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Anti-TNBC effects of MAG were assessed in MDA-MB-231 and 4T1 cells via viability, apoptosis, and ferroptosis assays (intracellular Fe\n                      <jats:sup>2+<\/jats:sup>\n                      , LPO, GSH). Target identification employed network pharmacology, RNA-seq, surface plasmon resonance, and pull-down assays. Mechanisms were validated using siRNA\/overexpression, Co-IP, and immunofluorescence.\n                      <jats:italic>In vivo<\/jats:italic>\n                      efficacy was evaluated in xenograft models.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      MAG inhibited proliferation and induced apoptosis and ferroptosis in TNBC cells, evidenced by elevated Fe\n                      <jats:sup>2+<\/jats:sup>\n                      and lipid peroxidation, depleted GSH, downregulated SLC7A11\/GPX4, and mitochondrial shrinkage effects reversed by ferroptosis inhibitors. MAG directly bound and suppressed transcription factor ETS2. ETS2 knockdown sensitized cells to MAG-induced ferroptosis, while its overexpression restored SLC7A11\/GPX4 expression and conferred resistance. Mechanistically, ETS2 transcriptionally regulated SLC7A11, and MAG enhanced ETS2-SLC7A11 protein interaction.\n                      <jats:italic>In vivo<\/jats:italic>\n                      , MAG significantly suppressed tumor growth with low toxicity and downregulated ETS2, SLC7A11, and GPX4.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MAG induces ferroptosis in MDA-MB-231 cells, which may be mediated by targeting the ETS2\/SLC7A11\/GPX4 signaling axis, providing mechanistic insights into its anti-TNBC activity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2026.1876648","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:39:03Z","timestamp":1785476343000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Magnolol triggers ferroptosis in triple-negative breast cancer through modulation of the ETS2\/SLC7A11\/GPX4 signaling axis"],"prefix":"10.3389","volume":"17","author":[{"given":"Jing","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Zhong","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhuang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Han","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]},{"name":"Anhui Province Biochemical Pharmaceutical Engineering Technology Research Center","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]},{"name":"Anhui Province Biochemical Pharmaceutical Engineering Technology Research Center","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine, Bengbu Medical University","place":["Bengbu, Anhui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo-Han","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]},{"name":"Anhui Province Biochemical Pharmaceutical Engineering Technology Research Center","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Zhu","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]},{"name":"Anhui Province Biochemical Pharmaceutical Engineering Technology Research Center","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Mei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Bengbu Medical University","place":["Bengbu, China"]},{"name":"Anhui Province Biochemical Pharmaceutical Engineering Technology Research Center","place":["Bengbu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2244","DOI":"10.1038\/s41467-021-22471-y","article-title":"Ferroptotic cell death triggered by conjugated linolenic acids is mediated by ACSL1","volume":"12","author":"Beatty","year":"2021","journal-title":"Nat. Commun."},{"key":"B2","doi-asserted-by":"publisher","first-page":"4963","DOI":"10.1038\/s41596-021-00615-0","article-title":"Analysis of clonogenic growth in vitro","volume":"16","author":"Brix","year":"2021","journal-title":"Nat. Protoc."},{"key":"B3","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/s11658-025-00785-9","article-title":"RSL3 promotes PARP1 apoptotic functions by distinct mechanisms during ferroptosis","volume":"30","author":"Chen","year":"2025","journal-title":"Cell. Mol. Biol. Lett"},{"key":"B4","doi-asserted-by":"publisher","first-page":"100975","DOI":"10.1016\/j.jpha.2024.100975","article-title":"Dissection of triple-negative breast cancer microenvironment and identification of potential therapeutic drugs using single-cell RNA sequencing analysis","volume":"14","author":"Cheng","year":"2024","journal-title":"J. Pharm. Anal."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1475","DOI":"10.3390\/biom13101475","article-title":"USP39-mediated non-proteolytic control of ETS2 suppresses nuclear localization and activity","volume":"13","author":"Choi","year":"2023","journal-title":"Biomolecules"},{"key":"B6","doi-asserted-by":"publisher","first-page":"925","DOI":"10.1038\/s41419-022-05384-6","article-title":"Ferroptosis: a double-edged sword mediating immune tolerance of cancer","volume":"13","author":"Dang","year":"2022","journal-title":"Cell Death Dis."},{"key":"B7","doi-asserted-by":"publisher","first-page":"1733","DOI":"10.1038\/s41401-025-01481-2","article-title":"Bardoxolone displays potent activity against triple negative breast cancer by inhibiting the TRIP13\/STAT3 circuit","volume":"46","author":"Deng","year":"2025","journal-title":"Acta. Pharmacol. Sin."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1060","DOI":"10.1016\/j.cell.2012.03.042","article-title":"Ferroptosis: an iron-dependent form of nonapoptotic cell death","volume":"149","author":"Dixon","year":"2012","journal-title":"Cell"},{"key":"B9","doi-asserted-by":"publisher","first-page":"3891","DOI":"10.1021\/acs.jcim.1c00203","article-title":"AutoDock vina 1.2.0: new docking methods, expanded force field, and python bindings","volume":"61","author":"Eberhardt","year":"2021","journal-title":"J. Chem. Inf. Model."},{"key":"B10","doi-asserted-by":"publisher","first-page":"102917","DOI":"10.1016\/j.redox.2023.102917","article-title":"Glutathione and peroxisome redox homeostasis","volume":"67","author":"Ferreira","year":"2023","journal-title":"Redox. Biol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1007\/s40259-022-00549-3","article-title":"Therapeutic siRNA: State-Of-The-Art and future perspectives","volume":"36","author":"Friedrich","year":"2022","journal-title":"BioDrugs"},{"key":"B12","doi-asserted-by":"publisher","first-page":"e833","DOI":"10.1002\/cpz1.833","article-title":"Western blotting with fast SDS-PAGE and semi-dry protein transfer","volume":"3","author":"Gari\u0107","year":"2023","journal-title":"Curr. Protoc."},{"key":"B13","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1097\/CM9.0000000000002239","article-title":"Regulated cell death in cancer: from pathogenesis to treatment","volume":"136","author":"Gong","year":"2023","journal-title":"Chin. Med. J. Engl."},{"key":"B14","doi-asserted-by":"publisher","first-page":"e889","DOI":"10.1002\/ctm2.889","article-title":"N6-methyladenosine-RNA methylation promotes expression of solute carrier family 7 member 11, an uptake transporter of cystine for lipid reactive oxygen species scavenger glutathione synthesis, leading to hepatoblastoma ferroptosis resistance","volume":"12","author":"Hill","year":"2022","journal-title":"Clin.Transl. Med."},{"key":"B15","doi-asserted-by":"publisher","first-page":"108592","DOI":"10.1016\/j.cclet.2023.108592","article-title":"Chemo-immunotherapy for chemo-resistance and metastasis of triple-negative breast cancer by combination of iron-oxide nanoparticles and dual targeting doxorubicin liposomes","volume":"34","author":"Hu","year":"2023","journal-title":"Chin. Chem. Lett."},{"key":"B16","doi-asserted-by":"publisher","first-page":"101510","DOI":"10.1016\/j.jcmgh.2025.101510","article-title":"The relationship between CSF1R signaling, monocyte-macrophage differentiation, and susceptibility to inflammatory bowel disease","volume":"19","author":"Hume","year":"2025","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"e1900325","DOI":"10.1002\/pmic.201900325","article-title":"Profiling the protein targets of unmodified bio-active molecules with drug affinity responsive target stability and liquid chromatography\/tandem mass spectrometry","volume":"20","author":"Hwang","year":"2020","journal-title":"Proteomics"},{"key":"B18","doi-asserted-by":"publisher","first-page":"2229","DOI":"10.1038\/s41401-024-01336-2","article-title":"The regulation and function of Nrf2 signaling in ferroptosis-activated cancer therapy","volume":"45","author":"Jiang","year":"2024","journal-title":"Acta. Pharmacol. Sin."},{"key":"B19","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/s40880-0180288-x","article-title":"Amino acid transporter SLC7A11\/xCT at the crossroads of regulating redox homeostasis and nutrient dependency of cancer","volume":"38","author":"Koppula","year":"2018","journal-title":"Cancer Commun."},{"key":"B20","doi-asserted-by":"publisher","first-page":"5566","DOI":"10.1021\/bi200642e","article-title":"Regulatory mechanisms of tumor suppressor P16INK4A and their relevance to cancer","volume":"50","author":"Li","year":"2011","journal-title":"Biochemistry"},{"key":"B21","doi-asserted-by":"publisher","first-page":"116925","DOI":"10.1016\/j.bcp.2025.116925","article-title":"Discovery of a novel BCL-2 inhibitor GW806742X for the treatment of TNBC","volume":"237","author":"Liu","year":"","journal-title":"Biochem. Pharmacol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"156925","DOI":"10.1016\/j.phymed.2025.156925","article-title":"Ponicidin triggered ferroptosis in esophageal squamous cell carcinoma by suppressing the SLC7A11\/Glutathione\/GPX4 signalling axis","volume":"143","author":"Liu","year":"","journal-title":"Phytomedicine"},{"key":"B23","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1016\/j.jep.2019.02.041","article-title":"A review of the phytochemistry and pharmacological activities of Magnoliae officinalis Cortex","volume":"236","author":"Luo","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1038\/nprot.2007.25","article-title":"Establishment of human tumor xenografts in immunodeficient mice","volume":"2","author":"Morton","year":"2007","journal-title":"Nat. Protoc."},{"key":"B25","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1021\/acs.jnatprod.9b01285","article-title":"Natural products as sources of new drugs over the nearly four decades from 01\/1981 to 09\/2019","volume":"83","author":"Newman","year":"2020","journal-title":"J. Nat. Prod."},{"key":"B26","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1038\/s43586-022-00131-9","article-title":"Volume electron microscopy","volume":"2","author":"Peddie","year":"2022","journal-title":"Nat. Rev. Methods Prim."},{"key":"B27","doi-asserted-by":"publisher","first-page":"e820","DOI":"10.1002\/ctm2.820","article-title":"Connecting N6\u2010methyladenosine modification to ferroptosis resistance in hepatoblastoma","volume":"12","author":"Peng","year":"2022","journal-title":"Clin. Transl. Med."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1426","DOI":"10.3390\/ijms23031426","article-title":"Ten approaches that improve immunostaining: a review of the latest advances for the optimization of immunofluorescence","volume":"23","author":"Pi\u00f1a","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1348","DOI":"10.1111\/iej.13353","article-title":"MTT versus other cell viability assays to evaluate the biocompatibility of root canal filling materials: a systematic review","volume":"53","author":"Pintor","year":"2020","journal-title":"Int. Endod. J."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1325347","DOI":"10.3389\/fcimb.2023.1325347","article-title":"Mechanistic insights of magnolol antimicrobial activity against mycoplasma using untargeted metabolomic analyses","volume":"13","author":"Qiao","year":"2023","journal-title":"Front. Cell. Infec. Microbiol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"102593","DOI":"10.1016\/j.redox.2022.102593","article-title":"Side-by-side comparison of recombinant human glutathione peroxidases identifies overlapping substrate specificities for soluble hydroperoxides","volume":"59","author":"Schwarz","year":"2023","journal-title":"Redox. Biol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1016\/j.chembiol.2024.09.008","article-title":"Lipid availability influences ferroptosis sensitivity in cancer cells by regulating polyunsaturated fatty acid trafficking","volume":"32","author":"Sokol","year":"2025","journal-title":"Cell Chem. Biol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1089\/dna.2025.0064","article-title":"The role of ETS2 in macrophage inflammation","volume":"44","author":"Stankey","year":"2025","journal-title":"DNA Cell Biol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1038\/s41586-024-07501-1","article-title":"A disease-associated gene desert directs macrophage inflammation through ETS2","volume":"630","author":"Stankey","year":"2024","journal-title":"Nature"},{"key":"B35","doi-asserted-by":"publisher","first-page":"2401","DOI":"10.1016\/j.cell.2022.06.003","article-title":"Ferroptosis turns 10: emerging mechanisms, physiological functions, and therapeutic applications","volume":"185","author":"Stockwell","year":"2022","journal-title":"Cell"},{"key":"B36","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1007\/978-1-0716-3327-4_15","article-title":"Characterization of small molecule-protein interactions using SPR method","volume":"2690","author":"Sun","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1007\/978-1-4939-7871-7_20","article-title":"Analysis of protein-protein interaction by Co-IP in human cells","volume":"1794","author":"Tang","year":"2018","journal-title":"Methods Mol. Biol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1038\/s41419-024-06903-3","article-title":"ID2-ETS2 axis regulates the transcriptional acquisition of pro-tumoral microglia phenotype in glioma","volume":"15","author":"V\u00e1zquez-Cabrera","year":"2024","journal-title":"Cell Death Dis."},{"key":"B39","doi-asserted-by":"publisher","first-page":"6441","DOI":"10.3390\/molecules27196441","article-title":"Magnolol as a potential anticancer agent: a proposed mechanistic insight","volume":"27","author":"Wang","year":"2022","journal-title":"Molecules"},{"key":"B40","doi-asserted-by":"publisher","first-page":"1712","DOI":"10.1038\/s41401-023-01062-1","article-title":"Aspirin triggers ferroptosis in hepatocellular carcinoma cells through restricting NF-\u03baB p65-activated SLC7A11 transcription","volume":"44","author":"Wang","year":"2023","journal-title":"Acta Pharmacol. Sin."},{"key":"B41","doi-asserted-by":"publisher","first-page":"216619","DOI":"10.1016\/j.canlet.2024.216619","article-title":"AMPK\u03b11-mediated ZDHHC8 phosphorylation promotes the palmitoylation of SLC7A11 to facilitate ferroptosis resistance in glioblastoma","volume":"584","author":"Wang","year":"2024","journal-title":"Cancer Lett."},{"key":"B42","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1007\/978-1-0716-1920-9_22","article-title":"RNA-Seq experiment and data analysis","volume":"2418","author":"Withanage","year":"2022","journal-title":"Methods Mol. Biol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"e0270599","DOI":"10.1371\/journal.pone.0270599","article-title":"Inducing apoptosis using chemical treatment and acidic pH, and detecting it using the Annexin V flow cytometric assay","volume":"17","author":"Worsley","year":"2022","journal-title":"PLoS One"},{"key":"B44","doi-asserted-by":"publisher","first-page":"156130","DOI":"10.1016\/j.phymed.2024.156130","article-title":"Bufalin induces ferroptosis by modulating the 2,4-dienoyl-CoA reductase (DECR1)-SLC7A11 axis in breast cancer","volume":"135","author":"Wu","year":"2024","journal-title":"Phytomedicine"},{"key":"B45","doi-asserted-by":"publisher","first-page":"156611","DOI":"10.1016\/j.phymed.2025.156611","article-title":"\u03b1-Hederin causes ferroptosis in triple-negative breast cancer through modulating IRF1 to suppress GPX4","volume":"141","author":"Wu","year":"2025","journal-title":"Phytomedicine"},{"key":"B46","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1038\/s41420-025-02308-z","article-title":"Targeting ferroptosis: a promising approach for treating lung carcinoma","volume":"11","author":"Wu","year":"2025","journal-title":"Cell Death Disco"},{"key":"B47","doi-asserted-by":"publisher","first-page":"102999","DOI":"10.1016\/j.redox.2023.102999","article-title":"Cysteine and homocysteine can be exploited by GPX4 in ferroptosis inhibition independent of GSH synthesis","volume":"69","author":"Xia","year":"2024","journal-title":"Redox Biol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"217697","DOI":"10.1016\/j.canlet.2025.217697","article-title":"Glutathione metabolism in ferroptosis and cancer therapy","volume":"621","author":"Xue","year":"2025","journal-title":"Cancer Lett."},{"key":"B49","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.cmet.2022.09.021","article-title":"Ferroptosis heterogeneity in triple-negative breast cancer reveals an innovative immunotherapy combination strategy","volume":"35","author":"Yang","year":"2023","journal-title":"Cell Metab."},{"key":"B50","doi-asserted-by":"publisher","first-page":"e2301292","DOI":"10.1002\/adhm.202301292","article-title":"Synergistic amplification of ferroptosis with liposomal oxidation catalyst and Gpx4 inhibitor for enhanced cancer therapy","volume":"12","author":"Yuan","year":"2023","journal-title":"Adv. Healthc. Mat."},{"key":"B51","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.biopha.2018.05.029","article-title":"Down-regulation of ETS2 inhibits the invasion and metastasis of renal cell carcinoma cells by inducing EMT via the PI3K\/Akt signaling pathway","volume":"104","author":"Zhang","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"B52","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1038\/s41401-022-00981-9","article-title":"Sorafenib triggers ferroptosis via inhibition of HBXIP\/SCD axis in hepatocellular carcinoma","volume":"44","author":"Zhang","year":"2023","journal-title":"Acta Pharmacol. Sin."},{"key":"B53","doi-asserted-by":"publisher","first-page":"1385565","DOI":"10.3389\/fphar.2024.1385565","article-title":"Induction of ferroptosis by natural products in non-small cell lung cancer: a comprehensive systematic review","volume":"15","author":"Zhang","year":"2024","journal-title":"Front. Pharmacol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"163576","DOI":"10.1016\/j.cej.2025.163576","article-title":"Alkaline phosphatase-responsive lipid-like polyphosphonitrile nanovesicles to enhance immunotherapy and inhibit metastasis of triple-negative breast cancer","volume":"515","author":"Zhao","year":"2025","journal-title":"Chem. Eng. J."},{"key":"B55","doi-asserted-by":"publisher","first-page":"2607","DOI":"10.3390\/cells10102607","article-title":"Tutorial: guidelines for single-cell RT-qPCR","volume":"10","author":"Zucha","year":"2021","journal-title":"Cells"}],"container-title":["Frontiers in Pharmacology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2026.1876648\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:39:04Z","timestamp":1785476344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2026.1876648\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,31]]},"references-count":55,"alternative-id":["10.3389\/fphar.2026.1876648"],"URL":"https:\/\/doi.org\/10.3389\/fphar.2026.1876648","relation":{},"ISSN":["1663-9812"],"issn-type":[{"value":"1663-9812","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,31]]},"article-number":"1876648"}}