{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:52:03Z","timestamp":1791348723498,"version":"4.3.1"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Nectin4 is an epithelial cell-adhesion molecule that may be involved in airway inflammatory responses. However, the effects of Nectin4 targeting in asthma has not yet been investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Here we engineered anti-Nectin4 monoclonal antibody, N-1T1C8Ab or N-1T3H9Ab, their effects on airway epithelial dysfunction and inflammation. Dermatophagoides pteronyssinus 1 (\n                      <jats:italic>Der p<\/jats:italic>\n                      1)\u2013stimulated normal human bronchial epithelial (NHBE) cells and ovalbumin-induced asthmatic BALB\/c mice were used as experimental models.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Anti-Nectin4 antibody treatment restored epithelial barrier dysfunction and was associated with changes in IL-33-related, MyD88\/IRAK, and Afadin\/Rac1 signaling in NHBE cells. In the murine asthma model, administration of N-1T1C8Ab or N-1T3H9Ab reduced airway inflammation, airway resistance, and immune cell infiltration in bronchoalveolar lavage fluid. Histological analysis demonstrated decreased inflammatory cell infiltration, goblet cell hyperplasia, and fibrosis in lung tissues. Anti-Nectin4 antibody treatment was also associated with modulation of IL-33\/MyD88-related signaling components.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings indicate that anti-Nectin4 monoclonal antibodies attenuate airway inflammation and improve epithelial barrier function. Further preclinical studies are warranted to determine the potential of Nectin4 targeting in asthma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2026.1931755","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:48:52Z","timestamp":1791348532000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti-Nectin4 monoclonal antibodies attenuate airway inflammation and epithelial barrier dysfunction in asthma"],"prefix":"10.3389","volume":"17","author":[{"given":"Pureun-Haneul","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital","place":["Bucheon, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MinHyeok","family":"An","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital","place":["Bucheon, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SangMin","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital","place":["Bucheon, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An-Soo","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital","place":["Bucheon, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"83","DOI":"10.3390\/ijms23010083","article-title":"Advances and challenges of antibody therapeutics for severe bronchial asthma","volume":"23","author":"Abe","year":"2021","journal-title":"Int. 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