{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T23:43:37Z","timestamp":1773359017178,"version":"3.50.1"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31302053"],"award-info":[{"award-number":["31302053"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872439"],"award-info":[{"award-number":["31872439"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"DOI":"10.3389\/fphys.2019.00819","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T11:44:01Z","timestamp":1561635841000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Identification and Functional Verification of MicroRNA-16 Family Targeting Intestinal Divalent Metal Transporter 1 (DMT1) in vitro and in vivo"],"prefix":"10.3389","volume":"10","author":[{"given":"Shuxia","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shihui","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huifang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingdong","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqiang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruqian","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2019,6,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.cell.2007.04.037","article-title":"Molecular basis for target RNA recognition and cleavage by human RISC.","volume":"130","author":"Ameres","year":"2007","journal-title":"Cell"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1244","DOI":"10.3324\/haematol.2009.020685","article-title":"Regulation of divalent metal transporter 1 (DMT1) non-IRE isoform by the microRNA Let-7d in erythroid cells.","volume":"95","author":"Andolfo","year":"2010","journal-title":"Haematologica"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2010.24","article-title":"Target mRNA abundance dilutes microRNA and siRNA activity.","volume":"6","author":"Arvey","year":"2010","journal-title":"Mol. Syst. Biol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1007\/s00109-015-1362-3","article-title":"MiR-20a regulates expression of the iron exporter ferroportin in lung cancer.","volume":"94","author":"Babu","year":"2016","journal-title":"J. Mol. Med."},{"key":"B5","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: target recognition and regulatory functions.","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"B6","first-page":"3964","article-title":"The Nramp2\/DMT1 iron transporter is induced in the duodenum of microcytic anemia mk mice but is not properly targeted to the intestinal brush border.","volume":"96","author":"Canonne-Hergaux","year":"2000","journal-title":"Blood"},{"key":"B7","doi-asserted-by":"crossref","first-page":"4406","DOI":"10.1182\/blood.V93.12.4406","article-title":"Cellular and subcellular localization of the Nramp2 iron transporter in the intestinal brush border and regulation by dietary iron.","volume":"93","author":"Canonne-Hergaux","year":"1999","journal-title":"Blood"},{"key":"B8","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.tcm.2012.05.003","article-title":"MicroRNA-503 and the extended microRNA-16 family in angiogenesis.","volume":"21","author":"Caporali","year":"2011","journal-title":"Trends Cardiovasc. Med."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1386","DOI":"10.1172\/jci44883","article-title":"The liver-specific microRNA miR-122 controls systemic iron homeostasis in mice.","volume":"121","author":"Castoldi","year":"2011","journal-title":"J. Clin. Invest."},{"key":"B10","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1007\/978-1-4939-2547-6_17","article-title":"Dual luciferase gene reporter assays to study miRNA function.","volume":"1296","author":"Clement","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"3687","DOI":"10.1073\/pnas.0511155103","article-title":"The colorectal microRNAome.","volume":"103","author":"Cummins","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B12","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/j.molcel.2016.09.027","article-title":"Impact of microRNA levels, target-site complementarity, and cooperativity on competing endogenous RNA-regulated gene expression.","volume":"64","author":"Denzler","year":"2016","journal-title":"Mol. Cell."},{"key":"B13","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1055\/s-0043-118910","article-title":"Effects of zinc alpha2 glycoprotein on lipid metabolism of liver in high-fat diet-induced obese mice.","volume":"49","author":"Fan","year":"2017","journal-title":"Horm. Metab. Res."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1073\/pnas.95.3.1148","article-title":"Nramp2 is mutated in the anemic Belgrade (b) rat: evidence of a role for Nramp2 in endosomal iron transport.","volume":"95","author":"Fleming","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B15","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1038\/ng0897-383","article-title":"Microcytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene.","volume":"16","author":"Fleming","year":"1997","journal-title":"Nat. Genet."},{"key":"B16","doi-asserted-by":"publisher","first-page":"4268","DOI":"10.1182\/blood-2008-04-150953","article-title":"Regulation of the divalent metal ion transporter DMT1 and iron homeostasis by a ubiquitin-dependent mechanism involving Ndfips and WWP2.","volume":"112","author":"Foot","year":"2008","journal-title":"Blood"},{"key":"B17","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1101\/gr.082701.108","article-title":"Most mammalian mRNAs are conserved targets of microRNAs.","volume":"19","author":"Friedman","year":"2009","journal-title":"Genome Res."},{"key":"B18","doi-asserted-by":"publisher","first-page":"844","DOI":"10.1016\/j.celrep.2013.02.026","article-title":"Iron regulatory proteins control a mucosal block to intestinal iron absorption.","volume":"3","author":"Galy","year":"2013","journal-title":"Cell Rep."},{"key":"B19","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/s1001-9294(10)60043-0","article-title":"Fatty acid synthase and hormone-sensitive lipase expression in liver are involved in zinc-alpha2-glycoprotein-induced body fat loss in obese mice.","volume":"25","author":"Gong","year":"2010","journal-title":"Chin. Med. Sci. J."},{"key":"B20","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.1038\/ijo.2009.141","article-title":"Zinc-alpha2-glycoprotein is involved in regulation of body weight through inhibition of lipogenic enzymes in adipose tissue.","volume":"33","author":"Gong","year":"2009","journal-title":"Int. J. Obes."},{"key":"B21","doi-asserted-by":"publisher","first-page":"D140","DOI":"10.1093\/nar\/gkj112","article-title":"MiRBase: microRNA sequences, targets and gene nomenclature.","volume":"34","author":"Griffiths-Jones","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"B22","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/s0014-5793(01)03189-1","article-title":"Iron-dependent regulation of the divalent metal ion transporter.","volume":"509","author":"Gunshin","year":"2001","journal-title":"FEBS Lett."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1258","DOI":"10.1172\/jci24356","article-title":"Slc11a2 is required for intestinal iron absorption and erythropoiesis but dispensable in placenta and liver.","volume":"115","author":"Gunshin","year":"2005","journal-title":"J. Clin. Invest."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03072-8","article-title":"Ultraconserved element uc.372 drives hepatic lipid accumulation by suppressing miR-195\/miR4668 maturation.","volume":"9","author":"Guo","year":"2018","journal-title":"Nat. Commun."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1910","DOI":"10.1038\/onc.2012.214","article-title":"MicroRNA-497 targets insulin-like growth factor 1 receptor and has a tumour suppressive role in human colorectal cancer.","volume":"32","author":"Guo","year":"2013","journal-title":"Oncogene"},{"key":"B26","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1016\/j.molmed.2014.06.005","article-title":"MicroRNAs in cancer: biomarkers, functions and therapy.","volume":"20","author":"Hayes","year":"2014","journal-title":"Trends Mol. Med."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1434.e15","DOI":"10.1053\/j.gastro.2017.01.049","article-title":"MicroRNA 301A promotes intestinal inflammation and colitis-associated cancer development by inhibiting BTG1.","volume":"152","author":"He","year":"2017","journal-title":"Gastroenterology"},{"key":"B28","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/j.tcb.2015.07.011","article-title":"The functions of microRNAs: mRNA decay and translational repression.","volume":"25","author":"Iwakawa","year":"2015","journal-title":"Trends Cell. Biol."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0020363","article-title":"Human microRNA targets.","volume":"2","author":"John","year":"2004","journal-title":"PLoS Biol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1278","DOI":"10.1038\/ng2135","article-title":"The role of site accessibility in microRNA target recognition.","volume":"39","author":"Kertesz","year":"2007","journal-title":"Nat. Genet."},{"key":"B31","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1038\/nbt.1543","article-title":"Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs.","volume":"27","author":"Khan","year":"2009","journal-title":"Nat. Biotechnol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"833","DOI":"10.3892\/mmr.2016.6088","article-title":"Influence of microRNA-34a on proliferation, invasion and metastasis of HCT116 cells.","volume":"15","author":"Li","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.3390\/nu9060617","article-title":"Long-term dexamethasone exposure down-regulates hepatic TFR1 and reduces liver iron concentration in rats.","volume":"9","author":"Li","year":"2017","journal-title":"Nutrients"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1458-8","article-title":"MiR-16 promotes the apoptosis of human cancer cells by targeting FEAT.","volume":"15","author":"Liang","year":"2015","journal-title":"BMC Cancer"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1368","DOI":"10.1002\/jcb.26297","article-title":"Knockdown of long non-coding RNA MALAT1 inhibits growth and motility of human hepatoma cells via modulation of miR-195.","volume":"119","author":"Liu","year":"2018","journal-title":"J. Cell. Biochem."},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00551","article-title":"MicroRNA-146a-5p mediates high glucose-induced endothelial inflammation via targeting interleukin-1 receptor-associated kinase 1 expression.","volume":"8","author":"Lo","year":"2017","journal-title":"Front. Physiol."},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00355","article-title":"MicroRNA-200a\/200b modulate high glucose-induced endothelial inflammation by targeting O-linked N-acetylglucosamine transferase expression.","volume":"9","author":"Lo","year":"2018","journal-title":"Front. Physiol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1172\/jci38499","article-title":"HIF-2alpha, but not HIF-1alpha, promotes iron absorption in mice.","volume":"119","author":"Mastrogiannaki","year":"2009","journal-title":"J. Clin. Invest."},{"key":"B39","doi-asserted-by":"publisher","first-page":"11171","DOI":"10.3390\/ijms140611171","article-title":"Fluctuation of global gene expression by endogenous miRNA response to the introduction of an exogenous miRNA.","volume":"14","author":"Nagata","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"B40","doi-asserted-by":"publisher","first-page":"G930","DOI":"10.1152\/ajpgi.2000.278.6.G930","article-title":"Gene expression of divalent metal transporter 1 and transferrin receptor in duodenum of Belgrade rats.","volume":"278","author":"Oates","year":"2000","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"B41","doi-asserted-by":"crossref","DOI":"10.1186\/s12974-016-0753-x","article-title":"In vivo inhibition of miR-155 significantly alters post-stroke inflammatory response.","volume":"13","author":"Pena-Philippides","year":"2016","journal-title":"J. Neuroinflam."},{"key":"B42","doi-asserted-by":"publisher","first-page":"D168","DOI":"10.1093\/nar\/gku988","article-title":"LncRNAdb v2.0: expanding the reference database for functional long noncoding RNAs.","volume":"43","author":"Quek","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1186\/1758-907x-3-3","article-title":"Target gene expression levels and competition between transfected and endogenous microRNAs are strong confounding factors in microRNA high-throughput experiments.","volume":"3","author":"Saito","year":"2012","journal-title":"Silence"},{"key":"B44","doi-asserted-by":"publisher","first-page":"18578","DOI":"10.1073\/pnas.0804373105","article-title":"Divalent metal transporter 1 (DMT1) contributes to neurodegeneration in animal models of Parkinson\u2019s disease.","volume":"105","author":"Salazar","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B45","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.cell.2011.07.014","article-title":"A ceRNA hypothesis: the rosetta stone of a hidden RNA language?","volume":"146","author":"Salmena","year":"2011","journal-title":"Cell"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003408","article-title":"Iron-responsive miR-485-3p regulates cellular iron homeostasis by targeting ferroportin.","volume":"9","author":"Sangokoya","year":"2013","journal-title":"PLoS Genet."},{"key":"B47","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.exphem.2008.10.002","article-title":"MiR-320 targets transferrin receptor 1 (CD71) and inhibits cell proliferation.","volume":"37","author":"Schaar","year":"2009","journal-title":"Exp. Hematol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/j.cmet.2008.12.012","article-title":"Intestinal hypoxia-inducible transcription factors are essential for iron absorption following iron deficiency.","volume":"9","author":"Shah","year":"2009","journal-title":"Cell Metab."},{"key":"B49","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1038\/nrg.2016.20","article-title":"Endogenous microRNA sponges: evidence and controversy.","volume":"17","author":"Thomson","year":"2016","journal-title":"Nat. Rev. Genet."},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2018.00165","article-title":"Lower expression of Ndfip1 is associated with Alzheimer disease pathogenesis through decreasing DMT1 degradation and increasing iron influx.","volume":"10","author":"Tian","year":"2018","journal-title":"Front. Aging Neurosci."},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-017-0204-2","article-title":"Negative effects of long-term feeding of high-grain diets to lactating goats on milk fat production and composition by regulating gene expression and DNA methylation in the mammary gland.","volume":"8","author":"Tian","year":"2017","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.29538","article-title":"Stress responsive miR-31 is a major modulator of mouse intestinal stem cells during regeneration and tumorigenesis.","volume":"6","author":"Tian","year":"2017","journal-title":"eLife"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032754","article-title":"Endocrine disruptor regulation of microRNA expression in breast carcinoma cells.","volume":"7","author":"Tilghman","year":"2012","journal-title":"PLoS One"},{"key":"B54","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1007\/s12672-016-0276-z","article-title":"MiR-15b inhibits the progression of glioblastoma cells through targeting insulin-like growth factor receptor 1.","volume":"8","author":"Wang","year":"2017","journal-title":"Horm Cancer"},{"key":"B55","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1667\/RR14428.1","article-title":"MiR-29a regulates radiosensitivity in human intestinal cells by targeting PTEN gene.","volume":"186","author":"Wang","year":"2016","journal-title":"Radiat. Res."},{"key":"B56","doi-asserted-by":"publisher","first-page":"1661","DOI":"10.1016\/j.biopha.2018.07.105","article-title":"LncRNA SNHG16 reverses the effects of miR-15a\/16 on LPS-induced inflammatory pathway.","volume":"106","author":"Wang","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"B57","doi-asserted-by":"publisher","first-page":"D146","DOI":"10.1093\/nar\/gku1104","article-title":"MiRDB: an online resource for microRNA target prediction and functional annotations.","volume":"43","author":"Wong","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"B58","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/j.cmet.2016.07.015","article-title":"Iron uptake via DMT1 integrates cell cycle with JAK-STAT3 signaling to promote colorectal tumorigenesis.","volume":"24","author":"Xue","year":"2016","journal-title":"Cell Metab."},{"key":"B59","doi-asserted-by":"publisher","first-page":"2295","DOI":"10.1097\/MIB.0b013e31829e71cf","article-title":"miR-106b fine tunes ATG16L1 expression and autophagic activity in intestinal epithelial HCT116 cells.","volume":"19","author":"Zhai","year":"2013","journal-title":"Inflamm. Bowel. Dis."},{"key":"B60","doi-asserted-by":"publisher","first-page":"4820","DOI":"10.12659\/msm.898574","article-title":"Expression of long non-coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) exacerbates hepatocellular carcinoma through suppressing miR-195.","volume":"22","author":"Zhang","year":"2016","journal-title":"Med. Sci. Monit."},{"key":"B61","doi-asserted-by":"publisher","first-page":"1867","DOI":"10.1159\/000485876","article-title":"MicroRNA-195 suppresses the progression of pancreatic cancer by targeting DCLK1.","volume":"44","author":"Zhou","year":"2017","journal-title":"Cell Physiol. Biochem."}],"container-title":["Frontiers in Physiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fphys.2019.00819\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T02:47:32Z","timestamp":1575168452000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fphys.2019.00819\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,27]]},"references-count":61,"alternative-id":["10.3389\/fphys.2019.00819"],"URL":"https:\/\/doi.org\/10.3389\/fphys.2019.00819","relation":{},"ISSN":["1664-042X"],"issn-type":[{"value":"1664-042X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,27]]},"article-number":"819"}}