{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T18:38:04Z","timestamp":1789670284266,"version":"4.0.1"},"reference-count":18,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to define the role of interleukine-1\u03b2 (IL-1\u03b2) and tumor necrosis factor-\u03b1 (TNF-\u03b1) in the expression of P311 in vascular endothelial cells (VECs) and in wound healing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>DAPI staining, a CCK-8 assay, cell migration assay, and an angiogenesis assay were used to assess the effects exerted by TNF-\u03b1 and IL-1\u03b2 at various concentrations on morphology, proliferation, migration, and angiogenesis of VECs. Western blot (WB) and reverse transcription-polymerase chain reaction (RT-PCR) models were employed to observe the effects exerted by proteins related to the nuclear factor-kappa B (NF-\u03baB) signaling pathway and P311 mRNA expression. Bioinformatics analysis was performed on the binding sites of P311 and NF-\u03baB. Finally, to investigate the effects of IL-1\u03b2 and TNF-\u03b1 on wound healing and the length of new epithelium in mice, we established a full-thickness wound defect model in mice. Immunohistochemistry was used to measure changes in P311, proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1\/CD31), as well as other related proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      When levels of TNF-\u03b1 and IL-1\u03b2 were both 20 ng\/ml, their effects on cell proliferation, cytoskeleton protein expression, cell migration, and angiogenesis were the greatest (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.05). IL-1\u03b2 and TNF-\u03b1 at moderate concentrations effectively promoted P311 mRNA and p-NF-\u03baB protein expression (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.05), while p-NF-K b protein expression was decreased (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.05). Luciferase assays showed that P311 expression was also relatively greater when stimulated at moderate concentrations (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.05), while relative expression was significantly lower when the p-NF-K b inhibitor CAPE was added (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.05). On 7-day wound healing rate comparison, the control, IL-1\u03b2, IL-1\u03b2ab, TNF-\u03b1, and TNF-\u03b1ab groups were 18, 37, 35, 39, and 36%, respectively, while control group + P311 siRNA was 31% (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.05). New epithelial length, granulation tissue thickness, and number of blood vessels trends were also the same. In the control group, P311 showed lower relative expression levels than the others (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.05). P311 relative expression levels trended as follows: control group &amp;gt; IL-1\u03b2ab &amp;gt; IL-1\u03b2 &amp;gt; TNF-\u03b1ab &amp;gt; TNF-\u03b1 (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      When IL-1\u03b2 and TNF-\u03b1 concentrations are moderate, they effectively promote the proliferation, expression, migration, and angiogenesis of VECs, possibly by promoting the expression of the NF-K b pathway and thereby promoting the expression of P311.\n                      <jats:italic>In vitro<\/jats:italic>\n                      experiments on mice suggest that P311 effectively promotes wound healing, and its mechanism may be closely related to PCNA, CD31, and VEGF.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphys.2020.545008","type":"journal-article","created":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T01:43:26Z","timestamp":1606441406000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":35,"title":["Effects of IL-1\u03b2 and TNF-\u03b1 on the Expression of P311 in Vascular Endothelial Cells and Wound Healing in Mice"],"prefix":"10.3389","volume":"11","author":[{"given":"Daijun","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengfei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weifeng","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoxing","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,11,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1038\/nrm2183","article-title":"Molecular regulation of angiogenesis and lymphangiogenesis.","volume":"8","author":"Adams","year":"2007","journal-title":"Nat. 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