{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T22:42:58Z","timestamp":1771368178727,"version":"3.50.1"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["307399\/2017-6"],"award-info":[{"award-number":["307399\/2017-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p><jats:bold>Aim:<\/jats:bold> We investigated the antioxidant protective power of egg white hydrolysate (EWH) against the vascular damage induced by mercury chloride (HgCl<jats:sub>2<\/jats:sub>) exposure in resistance arteries.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Male Wistar rats received for 60 days: (I) intramuscular injections (i.m.) of saline and tap water by gavage \u2013 Untreated group; (II) 4.6 \u03bcg\/kg of HgCl<jats:sub>2<\/jats:sub> i.m. for the first dose and subsequent doses of 0.07 \u03bcg\/kg\/day and tap water by gavage \u2013 HgCl<jats:sub>2<\/jats:sub> group; (III) saline i.m. and 1 g\/kg\/day of EWH by gavage \u2013 EWH group, or (IV) the combination of the HgCl<jats:sub>2<\/jats:sub> i.m. and EWH by gavage \u2013 EWH + HgCl<jats:sub>2<\/jats:sub> group. Blood pressure (BP) was indirectly measured and dose-response curves to acetylcholine (ACh), sodium nitroprusside (SNP), and noradrenaline (NE) were assessed in mesenteric resistance arteries (MRA), as <jats:italic>in situ<\/jats:italic> production of superoxide anion, nitric oxide (NO) release, vascular reactive oxygen species (ROS), lipid peroxidation, and antioxidant status.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Egg white hydrolysate prevented the elevation in BP and the vascular dysfunction after HgCl<jats:sub>2<\/jats:sub> exposure; restored the NO-mediated endothelial modulation and inhibited the oxidative stress and inflammatory pathways induced by HgCl<jats:sub>2<\/jats:sub>.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Egg white hydrolysate seems to be a useful functional food to prevent HgCl<jats:sub>2<\/jats:sub>-induced vascular toxic effects in MRA.<\/jats:p>","DOI":"10.3389\/fphys.2020.595767","type":"journal-article","created":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T05:38:01Z","timestamp":1605850681000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Antioxidant Properties of Egg White Hydrolysate Prevent Mercury-Induced Vascular Damage in Resistance Arteries"],"prefix":"10.3389","volume":"11","author":[{"given":"Alyne Goulart","family":"Escobar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danize Aparecida","family":"Rizzetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janaina Trindade","family":"Piagette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franck Maciel","family":"Pe\u00e7anha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalton Valentim","family":"Vassallo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Miguel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia Alessandra","family":"Wiggers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1161\/CIRCULATIONAHA.106.612325","article-title":"5-methyltetrahydrofolate rapidly improves endothelial function and decreases superoxide production in human vessels: effects on vascular tetrahydrobiopterin availability and endothelial nitric oxide synthase coupling","volume":"114","author":"Antoniades","year":"2006","journal-title":"Circulation"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1161\/HYPERTENSIONAHA.113.01991","article-title":"Increased nitric oxide bioavailability in adult GRK2 hemizygous mice protects against angiotensin II-induced hypertension","volume":"63","author":"Avenda\u00f1o","year":"2014","journal-title":"Hypertension"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: the FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. 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