{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T02:11:06Z","timestamp":1780711866394,"version":"3.54.1"},"reference-count":61,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["FS\/18\/41\/33762"],"award-info":[{"award-number":["FS\/18\/41\/33762"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p><jats:bold>Background and Purpose:<\/jats:bold> Arterial diameter is dictated by the contractile state of the vascular smooth muscle cells (VSMCs), which is modulated by direct and indirect inputs from endothelial cells (ECs). Modulators of KCNQ-encoded k<jats:sub>V<\/jats:sub>7 channels have considerable impact on arterial diameter and these channels are known to be expressed in VSMCs but not yet defined in ECs. However, expression of k<jats:sub>V<\/jats:sub>7 channels in ECs would add an extra level of vascular control. This study aims to characterize the expression and function of K<jats:sub>V<\/jats:sub>7 channels within rat mesenteric artery ECs.<\/jats:p><jats:p><jats:bold>Experimental Approach:<\/jats:bold> In rat mesenteric artery, KCNQ transcript and K<jats:sub>V<\/jats:sub>7 channel protein expression were determined via RT-qPCR, immunocytochemistry, immunohistochemistry and immunoelectron microscopy. Wire myography was used to determine vascular reactivity.<\/jats:p><jats:p><jats:bold>Key Results:<\/jats:bold> KCNQ transcript was identified in isolated ECs and VSMCs. K<jats:sub>V<\/jats:sub>7.1, K<jats:sub>V<\/jats:sub>7.4 and K<jats:sub>V<\/jats:sub>7.5 protein expression was determined in both isolated EC and VSMC and in whole vessels. Removal of ECs attenuated vasorelaxation to two structurally different K<jats:sub>V<\/jats:sub>7.2-5 activators S-1 and ML213. K<jats:sub>IR<\/jats:sub>2 blockers ML133, and BaCl<jats:sub>2<\/jats:sub> also attenuated S-1 or ML213-mediated vasorelaxation in an endothelium-dependent process. K<jats:sub>V<\/jats:sub>7 inhibition attenuated receptor-dependent nitric oxide (NO)-mediated vasorelaxation to carbachol, but had no impact on relaxation to the NO donor, SNP.<\/jats:p><jats:p><jats:bold>Conclusion and Implications:<\/jats:bold> In rat mesenteric artery ECs, K<jats:sub>V<\/jats:sub>7.4 and K<jats:sub>V<\/jats:sub>7.5 channels are expressed, functionally interact with endothelial K<jats:sub>IR<\/jats:sub>2.x channels and contribute to endogenous eNOS-mediated relaxation. This study identifies K<jats:sub>V<\/jats:sub>7 channels as novel functional channels within rat mesenteric ECs and suggests that these channels are involved in NO release from the endothelium of these vessels.<\/jats:p>","DOI":"10.3389\/fphys.2020.598779","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T06:25:40Z","timestamp":1607322340000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["KV7 Channel Expression and Function Within Rat Mesenteric Endothelial Cells"],"prefix":"10.3389","volume":"11","author":[{"given":"Samuel N.","family":"Baldwin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaun L.","family":"Sandow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gema","family":"Mond\u00e9jar-Parre\u00f1o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer B.","family":"Stott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain A.","family":"Greenwood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1635","DOI":"10.1111\/bph.14598","article-title":"TMEM16A is implicated in the regulation of coronary flow and is altered in hypertension.","volume":"176","author":"Askew Page","year":"2019","journal-title":"Br. 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