{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T13:41:32Z","timestamp":1778852492863,"version":"3.51.4"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:00:00Z","timestamp":1619395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>The <jats:italic>mdx<\/jats:italic> mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7\u201311-weeks old) <jats:italic>mdx<\/jats:italic> animals. The m<jats:italic>dx<\/jats:italic> mice were divided into three groups: <jats:italic>mdxSed<\/jats:italic>, sedentary controls (<jats:italic>n<\/jats:italic> = 28); <jats:italic>mdx<\/jats:italic>Ex, exercise-trained animals (<jats:italic>n<\/jats:italic> = 28); and <jats:italic>mdx<\/jats:italic>Ex+T, exercise-trained animals with the Tempol treatment (<jats:italic>n<\/jats:italic> = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-\u03baB and increased the PGC1-\u03b1 and PPAR\u03b4 levels in the exercise-trained-<jats:italic>mdx<\/jats:italic> mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD.<\/jats:p>","DOI":"10.3389\/fphys.2021.649793","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T06:03:40Z","timestamp":1619417020000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice"],"prefix":"10.3389","volume":"12","author":[{"given":"Heloina Nathalli\u00ea Mariano da","family":"Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Covatti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme Luiz da","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela Sayuri","family":"Mizobuti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael Dias","family":"M\u00e2ncio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00falio de Almeida","family":"Hermes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Akemi","family":"Kido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9ria Helena Alves","family":"Cagnon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine Cristina Leite","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine","family":"Minatel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,4,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1007\/s11010-015-2572-2","article-title":"Experimental evidence for the therapeutic potential of tempol in the treatment of acute liver injury.","volume":"411","author":"Abouzied","year":"2016","journal-title":"Mol. 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