{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:48:28Z","timestamp":1780678108637,"version":"3.54.1"},"reference-count":76,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5SC1GM125584"],"award-info":[{"award-number":["5SC1GM125584"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>Adult female mosquitoes rely on olfactory cues like carbon dioxide and other small molecules to find vertebrate hosts to acquire blood. The molecular physiology of the mosquito olfactory system is critical for their host preferences. Many laboratory strains of the yellow fever mosquito<jats:italic>Aedes aegypti<\/jats:italic>have been established since the late 19th century. These strains have been used for most molecular studies in this species. Some earlier comparative studies have identified significant physiological differences between different laboratory strains. In this study, we used a Y-tube olfactometer to determine the attraction of females of seven different strains of<jats:italic>Ae. aegypti<\/jats:italic>to a human host: UGAL, Rockefeller, Liverpool, Costa Rica, Puerto Rico, and two odorant receptor co-receptor (Orco) mutants Orco2 and Orco16. We performed RNA-seq using antennae of Rockefeller, Liverpool, Costa Rica, and Puerto Rico females. Our results showed that female<jats:italic>Aedes aegypti<\/jats:italic>from the Puerto Rico strain had significantly reduced attraction rates toward human hosts compared to all other strains. RNA-seq analyses of the antenna transcriptomes of Rockefeller, Liverpool, Costa Rica, and Puerto Rico strains revealed distinct differences in gene expression between the four strains, but conservation in gene expression patterns of known human-sensing genes. However, we identified several olfaction-related genes that significantly vary between strains, including receptors with significantly different expression in mosquitoes from the Puerto Rico strain and the other strains.<\/jats:p>","DOI":"10.3389\/fphys.2021.668236","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T11:52:56Z","timestamp":1629719576000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":19,"title":["Olfaction-Related Gene Expression in the Antennae of Female Mosquitoes From Common Aedes aegypti Laboratory Strains"],"prefix":"10.3389","volume":"12","author":[{"given":"Soumi","family":"Mitra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Pinch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yashoda","family":"Kandel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacy D.","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Immo A.","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1346","DOI":"10.1126\/science.161.3848.1346","article-title":"L-Lactic acid: a mosquito attractant isolated from humans.","volume":"161","author":"Acree","year":"1968","journal-title":"Science"},{"key":"B2","doi-asserted-by":"publisher","first-page":"5245","DOI":"10.1158\/0008-5472.can-04-0496","article-title":"Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets.","volume":"64","author":"Andersen","year":"2004","journal-title":"Cancer Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"e9471","DOI":"10.1371\/journal.pone.0009471","article-title":"The Anopheles gambiae odorant binding protein 1 (AgamOBP1) mediates indole recognition in the antennae of female mosquitoes.","volume":"5","author":"Biessmann","year":"2010","journal-title":"PLoS One"},{"key":"B4","doi-asserted-by":"publisher","first-page":"e7032","DOI":"10.1371\/journal.pone.0007032","article-title":"Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.","volume":"4","author":"Bohbot","year":"2009","journal-title":"PLoS One"},{"key":"B5","doi-asserted-by":"publisher","first-page":"e12138","DOI":"10.1371\/journal.pone.0012138","article-title":"Insect repellents: modulators of mosquito odorant receptor activity.","volume":"5","author":"Bohbot","year":"2010","journal-title":"PLoS One"},{"key":"B6","doi-asserted-by":"publisher","first-page":"39","DOI":"10.3389\/fevo.2015.00039","article-title":"The narrowing olfactory landscape of insect odorant receptors.","volume":"3","author":"Bohbot","year":"2015","journal-title":"Front. 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