{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T06:40:38Z","timestamp":1784097638972,"version":"3.55.0"},"reference-count":77,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T00:00:00Z","timestamp":1633478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>Bone-producing osteoblasts and dentin-producing odontoblasts are closely related cell types, a result from their shared evolutionary history in the ancient dermal skeleton. In mammals, the two cell types can be distinguished based on histological characters and the cells\u2019 position in the pulp cavity or in the tripartite periodontal complex. Different from mammals, teleost fish feature a broad diversity in tooth attachment modes, ranging from fibrous attachment to firm ankylosis to the underlying bone. The connection between dentin and jaw bone is often mediated by a collar of mineralized tissue, a part of the dental unit that has been termed \u201cbone of attachment\u201d. Its nature (bone, dentin, or an intermediate tissue type) is still debated. Likewise, there is a debate about the nature of the cells secreting this tissue: osteoblasts, odontoblasts, or yet another (intermediate) type of scleroblast. Here, we use expression of the P\/Q rich secretory calcium-binding phosphoprotein 5 (<jats:italic>scpp5<\/jats:italic>) to characterize the cells lining the so-called bone of attachment in the zebrafish dentition.<jats:italic>scpp5<\/jats:italic>is expressed in late cytodifferentiation stage odontoblasts but not in the cells depositing the \u201cbone of attachment\u201d. nor in<jats:italic>bona fide<\/jats:italic>osteoblasts lining the supporting pharyngeal jaw bone. Together with the presence of the osteoblast marker Zns-5, and the absence of covering epithelium, this links the cells depositing the \u201cbone of attachment\u201d to osteoblasts rather than to odontoblasts. The presence of dentinal tubule-like cell extensions and the near absence of osteocytes, nevertheless distinguishes the \u201cbone of attachment\u201d from true bone. These results suggest that the \u201cbone of attachment\u201d in zebrafish has characters intermediate between bone and dentin, and, as a tissue, is better termed \u201cdentinous bone\u201d. In other teleosts, the tissue may adopt different properties. The data furthermore support the view that these two tissues are part of a continuum of mineralized tissues. Expression of<jats:italic>scpp5<\/jats:italic>can be a valuable tool to investigate how differentiation pathways diverge between osteoblasts and odontoblasts in teleost models and help resolving the evolutionary history of tooth attachment structures in actinopterygians.<\/jats:p>","DOI":"10.3389\/fphys.2021.723210","type":"journal-article","created":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T22:03:24Z","timestamp":1633557804000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Cells at the Edge: The Dentin\u2013Bone Interface in Zebrafish Teeth"],"prefix":"10.3389","volume":"12","author":[{"given":"Joana T.","family":"Rosa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul Eckhard","family":"Witten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Huysseune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,10,6]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"2169","DOI":"10.1002\/jcp.27068","article-title":"The emerging role of extracellular Ca2+ in osteo\/odontogenic differentiation and the involvement of intracellular Ca2+ signaling: from osteoblastic cells to dental pulp cells and odontoblasts","volume":"234","author":"An","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1016\/j.devcel.2017.10.015","article-title":"Osteoblast production by reserved progenitor cells in zebrafish bone regeneration and maintenance","volume":"43","author":"Ando","year":"2017","journal-title":"Dev. Cell"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"247","DOI":"10.2174\/1574888X11666161026145149","article-title":"A perspective on stem cells as biological systems that produce differentiated osteoblasts and odontoblasts","volume":"12","author":"Ariffin","year":"2017","journal-title":"Curr. Stem Cell Res. Ther."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.zool.2005.09.004","article-title":"Structure, attachment, replacement and growth of teeth in bluefish, Pomatomus saltatrix (Linnaeus, 1766), a teleost with deeply socketed teeth","volume":"108","author":"Bemis","year":"2005","journal-title":"Zoology"},{"key":"ref5","volume-title":"Chondroid Bone, Secondary Cartilage and Metaplasia.","author":"Beresford","year":"1981"},{"key":"ref6","volume-title":"The Teeth of Non-Mammalian Vertebrates.","author":"Berkovitz","year":"2016"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1630","DOI":"10.3389\/fphys.2018.01630","article-title":"Current perspectives on tooth implantation, attachment, and replacement in Amniota","volume":"9","author":"Bertin","year":"2018","journal-title":"Front. Physiol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1111\/j.1525-142X.2006.00084.x","article-title":"Expression of dlx genes in zebrafish tooth development: evolutionary implications","volume":"8","author":"Borday-Birraux","year":"2006","journal-title":"Evol. Dev."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1177\/154405910508400501","article-title":"Are cementoblasts a subpopulation of osteoblasts or a unique phenotype?","volume":"84","author":"Bosshardt","year":"2005","journal-title":"J. Dent. Res."},{"key":"ref10","first-page":"25","article-title":"Ontogenetic aspects of dental evolution","volume":"39","author":"Butler","year":"1995","journal-title":"Int. J. Dev. Biol."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/S0940-9602(97)80099-4","article-title":"The development of the dentigerous bones and teeth in the hemiramphid fish Dermogenys pusillus (Atheriniformes, Teleostei)","volume":"179","author":"Clemen","year":"1997","journal-title":"Ann. Anat."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.bone.2015.01.006","article-title":"Identification of novel osteogenic compounds by an ex-vivo sp7:luciferase zebrafish scale assay","volume":"74","author":"de Vrieze","year":"2015","journal-title":"Bone"},{"key":"ref13","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1002\/dvg.20639","article-title":"Zebrafish sp7:EGFP: a transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration","volume":"48","author":"DeLaurier","year":"2010","journal-title":"genesis"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1002\/jemt.10217","article-title":"Origin and early evolution of vertebrate skeletonization","volume":"59","author":"Donoghue","year":"2002","journal-title":"Microsc. Res. Tech."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1002\/jmor.1051670203","article-title":"Ontogeny and phylogeny of tooth attachment modes in actinopterygian fishes","volume":"167","author":"Fink","year":"1981","journal-title":"J. Morphol."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1177\/0022034509355440","article-title":"Formation of the tooth-bone interface","volume":"89","author":"Fleischmannova","year":"2010","journal-title":"J. Dent. Res."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"3166","DOI":"10.1002\/dvdy.20957","article-title":"A hierarchy of Runx transcription factors modulate the onset of chondrogenesis in craniofacial endochondral bones in zebrafish","volume":"235","author":"Flores","year":"2006","journal-title":"Dev. Dyn."},{"key":"ref18","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1002\/dvdy.20603","article-title":"Buried alive: how osteoblasts become osteocytes","volume":"235","author":"Franz-Odendaal","year":"2006","journal-title":"Dev. Dyn."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1017\/CBO9780511542626.012","article-title":"Chapter 12. Evolution of tooth attachment in lower vertebrates to tetrapods","volume-title":"Development, Function and Evolution of Teeth.","author":"Gaengler","year":"2000"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"20190401","DOI":"10.1098\/rspb.2019.0401","article-title":"The first formed tooth serves as a signalling centre to induce the formation of the dental row in zebrafish","volume":"286","author":"Gibert","year":"2019","journal-title":"Proc. R. Soc. B"},{"key":"ref21","first-page":"13","article-title":"Plasticity of and transitions between skeletal tissues in vertebrate evolution and development","volume-title":"Major Transitions in Vertebrate Evolution.","author":"Hall","year":"2007"},{"key":"ref22","first-page":"126","article-title":"Plasticity and variation of skeletal cells and tissues and the evolutionary development of actinopterygian fishes","volume-title":"Evolution and Development of Fishes.","author":"Hall","year":"2019"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"3991","DOI":"10.1242\/dev.042150","article-title":"Two populations of endochondral osteoblasts with differential sensitivity to hedgehog signalling","volume":"136","author":"Hammond","year":"2009","journal-title":"Development"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1007\/BF00193129","article-title":"Structure and origin of the tooth pedicel (the so-called bone of attachment) and dental-ridge bone in the mandibles of the sea breams Acanthopagrus australis, Pagrus auratus and Rhabdosargus sarba (Sparidae, Perciformes, Teleostei)","volume":"189","author":"Hughes","year":"1994","journal-title":"Anat. Embryol."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1002\/jmor.1051750302","article-title":"Observations on tooth development and implantation in the upper pharyngeal jaws in Astatotilapia elegans (Trewavas, 1933; Teleostei: Cichlidae)","volume":"175","author":"Huysseune","year":"1983","journal-title":"J. Morphol."},{"key":"ref26","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/B978-012529650-2\/50024-X","article-title":"Skeletal system","volume-title":"The Laboratory Fish.","author":"Huysseune","year":"2000"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1002\/ar.1092330304","article-title":"Development of cartilage and bone tissues of the anterior part of the mandible in cichlid fish: a light and TEM study","volume":"233","author":"Huysseune","year":"1992","journal-title":"Anat. Rec."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1007\/s00427-008-0237-9","article-title":"Unique and shared gene expression patterns in Atlantic salmon (Salmo salar) tooth development","volume":"218","author":"Huysseune","year":"2008","journal-title":"Dev. Genes Evol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1007\/s004290050185","article-title":"Early development of the zebrafish (Danio rerio) pharyngeal dentition (Teleostei, Cyprinidae)","volume":"198","author":"Huysseune","year":"1998","journal-title":"Anat. Embryol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1111\/j.1525-142X.2007.00209.x","article-title":"An evolutionary view on tooth development and replacement in wild Atlantic salmon (Salmo salar L.)","volume":"10","author":"Huysseune","year":"2008","journal-title":"Evol. Dev."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1093\/genetics\/141.4.1583","article-title":"Temperature-sensitive mutations that cause stage-specific defects in zebrafish fin regeneration","volume":"141","author":"Johnson","year":"1995","journal-title":"Genetics"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"e47394","DOI":"10.1371\/journal.pone.0047394","article-title":"Skeletogenic fate of zebrafish cranial and trunk neural crest","volume":"7","author":"Kague","year":"2012","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/j.ydbio.2018.01.021","article-title":"Zebrafish sp7 mutants show tooth cycling independent of attachment, eruption and poor differentiation of teeth","volume":"435","author":"Kague","year":"2018","journal-title":"Dev. Biol."},{"key":"ref34","doi-asserted-by":"publisher","first-page":"3165","DOI":"10.1093\/molbev\/msaa153","article-title":"A genomic cluster containing novel and conserved genes is associated with cichlid fish dental developmental convergence","volume":"37","author":"Karagic","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1007\/s00427-009-0276-x","article-title":"The SCPP gene repertoire in bony vertebrates and graded differences in mineralized tissues","volume":"219","author":"Kawasaki","year":"2009","journal-title":"Dev. Genes Evol."},{"key":"ref36","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1159\/000324225","article-title":"The SCPP gene family and the complexity of hard tissues in vertebrates","volume":"194","author":"Kawasaki","year":"2011","journal-title":"Cells Tissues Organs"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1146\/annurev-genet-102108-134242","article-title":"Biomineralization in humans: making the hard choices in life","volume":"43","author":"Kawasaki","year":"2009","journal-title":"Annu. Rev. Genet."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"102023","DOI":"10.1016\/j.isci.2020.102023","article-title":"Coevolution of enamel, ganoin, enameloid, and their matrix SCPP genes in osteichthyans","volume":"24","author":"Kawasaki","year":"2021","journal-title":"iScience"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1002\/jez.b.22755","article-title":"SCPP genes and their relatives in gar: rapid expansion of mineralization genes in osteichthyans","volume":"328","author":"Kawasaki","year":"2017","journal-title":"J. Exp. Zool. B Mol. Dev. Evol."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1002\/jez.b.21088","article-title":"Evolutionary genetics of vertebrate tissue mineralization: the origin and evolution of the secretory calcium-binding phosphoprotein family","volume":"306","author":"Kawasaki","year":"2006","journal-title":"J. Exp. Zool. B Mol. Dev. Evol."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1177\/154405910808700608","article-title":"SCPP gene evolution and the dental mineralization continuum","volume":"87","author":"Kawasaki","year":"2008","journal-title":"J. Dent. Res."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1038\/s41559-018-0624-1","article-title":"The nature of aspidin and the evolutionary origin of bone","volume":"2","author":"Keating","year":"2018","journal-title":"Nat. Ecol. Evol."},{"key":"ref43","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1139\/z79-089","article-title":"The attachment of teeth in Lophius","volume":"57","author":"Kerebel","year":"1979","journal-title":"Can. J. Zool."},{"key":"ref44","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1111\/j.1469-7998.1960.tb05570.x","article-title":"Development and structure of some actinopterygian and urodele teeth","volume":"133","author":"Kerr","year":"1960","journal-title":"Proc. Zool. Soc. London"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1002\/aja.1002030302","article-title":"Stages of embryonic development of the zebrafish","volume":"203","author":"Kimmel","year":"1995","journal-title":"Dev. Dyn."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1016\/j.devcel.2011.04.014","article-title":"Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin","volume":"20","author":"Knopf","year":"2011","journal-title":"Dev. Cell"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"1694","DOI":"10.3390\/ijms20071694","article-title":"Regulation of proliferation, differentiation and functions of osteoblasts by Runx2","volume":"20","author":"Komori","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"ref48","doi-asserted-by":"publisher","first-page":"1021","DOI":"10.1002\/dvdy.300","article-title":"Miniaturization: how many cells are needed to build a tooth?","volume":"250","author":"Larionova","year":"2021","journal-title":"Dev. Dyn."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1002\/dvdy.21838","article-title":"Tracking gene expression during zebrafish osteoblast differentiation","volume":"238","author":"Li","year":"2009","journal-title":"Dev. Dyn."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/s12903-018-0680-6","article-title":"The role of osteomodulin on osteo\/odontogenic differentiation in human dental pulp stem cells","volume":"19","author":"Lin","year":"2019","journal-title":"BMC Oral Health"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1159\/000141794","article-title":"Studies of the acellular bone of teleost fish. I. Morphological and systematic variations","volume":"46","author":"Moss","year":"1961","journal-title":"Acta Anat."},{"key":"ref52","first-page":"481","article-title":"On the teeth of the larval Belone vulgaris, and the attachment of teeth in fishes","volume":"76","author":"Moy-Thomas","year":"1934","journal-title":"Quart. J. Micr. Sci."},{"key":"ref53","first-page":"321","article-title":"Histologic studies of placoderms and fossil elasmobranchs. 1. The endoskeleton, with remarks on the hard tissues of lower vertebrates in general","volume":"2","author":"\u00d8rvig","year":"1951","journal-title":"Ark. Zool."},{"key":"ref54","first-page":"45","article-title":"Phylogeny of tooth tissues: evolution of some calcified tissues in early vertebrates","volume-title":"Structural and Chemical Organization of Teeth.","author":"\u00d8rvig","year":"1967"},{"key":"ref55","first-page":"549","article-title":"From reptile to mammal: evolutionary considerations of the dentition with emphasis on tooth attachment","volume":"52","author":"Osborn","year":"1984","journal-title":"Symp. Zool. Soc. Lond."},{"key":"ref56","volume-title":"Comparative Odontology.","author":"Peyer","year":"1968"},{"key":"ref57","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1002\/dvdy.21836","article-title":"Osterix-mcherry transgenic medaka for in vivo imaging of bone formation","volume":"238","author":"Renn","year":"2009","journal-title":"Dev. Dyn."},{"key":"ref58","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1083\/jcb.131.2.483","article-title":"Osteogenic differentiation of hypertrophic chondrocytes involves asymmetric cell division and apoptosis","volume":"131","author":"Roach","year":"1995","journal-title":"J. Cell Biol."},{"key":"ref59","author":"Romeis","year":"1989"},{"key":"ref60","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1016\/j.bbagen.2016.03.034","article-title":"Central role of betaine-homocysteine S-methyltransferase 3 in chondral ossification and evidence for sub-functionalization in neoteleost fish","volume":"1860","author":"Rosa","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"ref61","first-page":"3","article-title":"Chapter 1. Comparative anatomy of tooth attachment","volume-title":"The Periodontal Ligament in Health and Disease.","author":"Shellis","year":"1982"},{"key":"ref62","doi-asserted-by":"publisher","first-page":"178","DOI":"10.3389\/fcell.2019.00178","article-title":"Collagen dynamics during the process of osteocyte embedding and mineralization","volume":"7","author":"Shiflett","year":"2019","journal-title":"Front. Cell Dev. Biol."},{"key":"ref63","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1002\/jemt.10220","article-title":"First-generation teeth in nonmammalian lineages: evidence for a conserved ancestral character?","volume":"59","author":"Sire","year":"2002","journal-title":"Microsc. Res. Tech."},{"key":"ref64","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1017\/S1464793102006073","article-title":"Formation of dermal skeletal and dental tissues in fish: a comparative and evolutionary approach","volume":"78","author":"Sire","year":"2003","journal-title":"Biol. Rev."},{"key":"ref65","first-page":"3","article-title":"Origin and evolution of bone and dentin and of acidic secretory calcium-binding phosphoproteins","volume-title":"Phosphorylated Extracellular Matrix Proteins of Bone and Dentin.","author":"Sire","year":"2012"},{"key":"ref66","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1111\/j.1463-6395.1994.tb01211.x","article-title":"The canaliculi of Williamson in holostean bone (Osteichthyes, Actinopterygii): a structural and ultrastructural study","volume":"75","author":"Sire","year":"1994","journal-title":"Acta Zool."},{"key":"ref67","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1007\/978-1-4615-2878-4_10","article-title":"A developmental model for evolution of the vertebrate exoskeleton and teeth. The role of cranial and trunk neural crest","volume-title":"Evolutionary Biology.","author":"Smith","year":"1993"},{"key":"ref68","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/jmor.1051270102","article-title":"Tooth attachment by means of a periodontium in the triggerfish (Balistidae)","volume":"127","author":"Soule","year":"1969","journal-title":"J. Morphol."},{"key":"ref69","first-page":"41","article-title":"Studies upon the attachment of teeth","volume":"7","author":"Tomes","year":"1875","journal-title":"Trans. Odontol. Soc."},{"key":"ref70","doi-asserted-by":"crossref","DOI":"10.5962\/bhl.title.20637","volume-title":"A Manual of Dental Anatomy: Human and Comparative.","author":"Tomes","year":"1882"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1643\/0045-8511(2001)001[0035:PODRTI]2.0.CO;2","article-title":"Position of developing replacement teeth in teleosts","volume":"2001","author":"Trapani","year":"2001","journal-title":"Copeia"},{"key":"ref72","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1002\/1097-0177(2000)9999:9999<::AID-DVDY1069>3.0.CO;2-Z","article-title":"Dynamics of tooth formation and replacement in the zebrafish (Danio rerio) (Teleostei, Cyprinidae)","volume":"219","author":"Van der heyden","year":"2000","journal-title":"Dev. Dyn."},{"key":"ref73","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1007\/s004410000180","article-title":"Development and fine structure of pharyngeal replacement teeth in juvenile zebrafish (Danio rerio) (Teleostei, Cyprinidae)","volume":"302","author":"Van der heyden","year":"2000","journal-title":"Cell Tissue Res."},{"key":"ref74","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1007\/978-1-61779-860-3_16","article-title":"Whole mount immunohistochemistry and in situ hybridization of larval and adult zebrafish dental tissues","volume":"887","author":"Verstraeten","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"ref75","volume-title":"The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish Danio (","author":"Westerfield","year":"2000"},{"key":"ref76","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.jdsr.2016.04.002","article-title":"Histology of human cementum: its structure, function, and development","volume":"52","author":"Yamamoto","year":"2016","journal-title":"Jpn. Dent. Sci. Rev."},{"key":"ref77","doi-asserted-by":"publisher","first-page":"12097","DOI":"10.1073\/pnas.1302703111","article-title":"Hypertrophic chondrocytes can become osteoblasts and osteocytes in endochondral bone formation","volume":"111","author":"Yang","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. 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