{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T18:12:23Z","timestamp":1767204743052,"version":"build-2238731810"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T00:00:00Z","timestamp":1640304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000156","name":"Fonds de Recherche du Qu\u00e9bec - Sant\u00e9","doi-asserted-by":"publisher","award":["268346"],"award-info":[{"award-number":["268346"]}],"id":[{"id":"10.13039\/501100000156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000156","name":"Fonds de Recherche du Qu\u00e9bec - Sant\u00e9","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>\n                    Excessive carotid body responsiveness to O\n                    <jats:sub>2<\/jats:sub>\n                    and\/or CO\n                    <jats:sub>2<\/jats:sub>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    stimuli contributes to respiratory instability and apneas during sleep. In hypogonadal men, testosterone supplementation may increase the risk of sleep-disordered breathing; however, the site of action is unknown. The present study tested the hypothesis that testosterone supplementation potentiates carotid body responsiveness to hypoxia in adult male rats. Because testosterone levels decline with age, we also determined whether these effects were age-dependent.\n                    <jats:italic>In situ<\/jats:italic>\n                    hybridization determined that androgen receptor mRNA was present in the carotid bodies and caudal nucleus of the solitary tract of adult (69 days old) and aging (193\u2013206 days old) male rats. In urethane-anesthetized rats injected with testosterone propionate (2 mg\/kg; i.p.), peak breathing frequency measured during hypoxia (FiO\n                    <jats:sub>2<\/jats:sub>\n                    = 0.12) was 11% greater vs. the vehicle treatment group. Interestingly, response intensity following testosterone treatment was positively correlated with animal age. Exposing\n                    <jats:italic>ex vivo<\/jats:italic>\n                    carotid body preparations from young and aging rats to testosterone (5 nM, free testosterone) 90\u2013120 min prior to testing showed that the carotid sinus nerve firing rate during hypoxia (5% CO\n                    <jats:sub>2<\/jats:sub>\n                    + 95% N\n                    <jats:sub>2<\/jats:sub>\n                    ; 15 min) was augmented in both age groups as compared to vehicle (&amp;lt;0.001% DMSO). Ventilatory measurements performed using whole body plethysmography revealed that testosterone supplementation (2 mg\/kg; i.p.) 2 h prior reduced apnea frequency during sleep. We conclude that in healthy rats, age-dependent potentiation of the carotid body\u2019s response to hypoxia by acute testosterone supplementation does not favor the occurrence of apneas but rather appears to stabilize breathing during sleep.\n                  <\/jats:p>","DOI":"10.3389\/fphys.2021.781662","type":"journal-article","created":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T03:47:48Z","timestamp":1640317668000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Testosterone Supplementation Induces Age-Dependent Augmentation of the Hypoxic Ventilatory Response in Male Rats With Contributions From the Carotid Bodies"],"prefix":"10.3389","volume":"12","author":[{"given":"Tara A.","family":"Janes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danuzia","family":"Ambrozio-Marques","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e9bastien","family":"Fournier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Soliz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Kinkead","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,12,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1016\/j.smrv.2007.12.003","article-title":"The effects of testosterone on sleep and sleep-disordered breathing in men: its bidirectional interaction with erectile function.","volume":"12","author":"Andersen","year":"2008","journal-title":"Sleep Med. 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