{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:16:15Z","timestamp":1792084575935,"version":"4.4.0"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>Peripheral arterial disease (PAD) is a common circulatory problem in lower extremities, and the murine ischemic model is used to reproduce human PAD. To compare strain differences of skeletal muscle responses to ischemia, the left femoral artery was blocked by ligation to reduce blood flow to the limb of BALB\/c and C57BL\/6 mice. After 6\u00a0weeks of the femoral artery ligation, the functional and morphological changes of the gastrocnemius muscle were evaluated. BALB\/c mice displayed serious muscular dystrophy, including smaller myofibers (524.3 \u00b1 66\u00a0\u00b5M<jats:sup>2<\/jats:sup>), accumulation of adipose-liked tissue (17.8 \u00b1 0.9%), and fibrosis (6.0 \u00b1 0.5%), compared to C57BL\/6 mice (1,328.3 \u00b1 76.3\u00a0\u00b5M<jats:sup>2<\/jats:sup>, 0.27 \u00b1 0.09%, and 1.56 \u00b1 0.06%, respectively; <jats:italic>p<\/jats:italic> &amp;lt; 0.05). About neuromuscular junctions (NMJs) in the gastrocnemius muscle, 6\u00a0weeks of the femoral artery ligation induced more damage in BALB\/c mice than that in C57BL\/6 mice, demonstrated by the fragment number of nicotinic acetylcholine receptor (nAChR) clusters (8.8 \u00b1 1.3 in BALB\/c vs. 2.5 \u00b1 0.7 in C57BL\/6 mice, <jats:italic>p<\/jats:italic> &amp;lt; 0.05) and amplitude of sciatic nerve stimulated-endplate potentials (EPPs) (9.29 \u00b1 1.34\u00a0mV in BALB\/c vs. 20.28 \u00b1 1.42\u00a0mV in C57BL\/6 mice, <jats:italic>p<\/jats:italic> &amp;lt; 0.05). More importantly, 6\u00a0weeks of the femoral artery ligation significantly weakened sciatic nerve-stimulated skeletal muscle contraction in BALB\/c mice, whereas it didn\u2019t alter the skeletal muscle contraction in C57BL\/6 mice. These results suggest that the femoral artery ligation in BALB\/c mice is a useful animal model to develop new therapeutic approaches to improve limb structure and function in PAD, although the mechanisms about strain differences of skeletal muscle responses to ischemia are unclear.<\/jats:p>","DOI":"10.3389\/fphys.2022.1014744","type":"journal-article","created":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T08:03:51Z","timestamp":1663315431000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Different responses of skeletal muscles to femoral artery ligation-induced ischemia identified in BABL\/c and C57BL\/6 mice"],"prefix":"10.3389","volume":"13","author":[{"given":"Huiyin","family":"Tu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junliang","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongze","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron N.","family":"Barksdale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael C.","family":"Wadman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iraklis I.","family":"Pipinos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Long","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"E3704","DOI":"10.3390\/ijms20153704","article-title":"Variations in surgical procedures for inducing hind limb ischemia in mice and the impact of these variations on neovascularization assessment","volume":"20","author":"Aref","year":"2019","journal-title":"Int. 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