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For instance, tumor metabolism shifts towards an enhanced glycolytic state even under aerobic conditions. In contrast, DIO triggers lipid-induced IR by impairing insulin signaling and reducing insulin-stimulated glucose uptake. Based on physiological differences in systemic metabolism, we used a breath analysis approach to discriminate between different pathological states using glucose oxidation as a readout. We assessed glucose utilization in lung cancer-induced cachexia and DIO mouse models using a U-<jats:sup>13<\/jats:sup>C glucose tracer and stable isotope sensors integrated into an indirect calorimetry system. Our data showed increased <jats:sup>13<\/jats:sup>CO<jats:sub>2<\/jats:sub> expired by tumor-bearing (TB) mice and a reduction in exhaled <jats:sup>13<\/jats:sup>CO<jats:sub>2<\/jats:sub> in the DIO model. Taken together, our findings illustrate high glucose uptake and consumption in TB animals and decreased glucose uptake and oxidation in obese mice with an IR phenotype. Our work has important translational implications for the utility of stable isotopes in breath-based detection of glucose homeostasis in models of lung cancer progression and DIO.<\/jats:p>","DOI":"10.3389\/fphys.2022.1023614","type":"journal-article","created":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T08:36:13Z","timestamp":1664958973000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Exogenous detection of 13C-glucose metabolism in tumor and diet-induced obesity models"],"prefix":"10.3389","volume":"13","author":[{"given":"Amandine","family":"Verlande","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Kook","family":"Chun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei A.","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Oettler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harm J.","family":"Knot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma","family":"Masri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"046002","DOI":"10.1088\/1752-7163\/aacf12","article-title":"The characterisation of hepatic mitochondrial function in patients with non-alcoholic fatty liver disease (NAFLD) using the 13 C-ketoisocaproate breath test","volume":"12","author":"Afolabi","year":"2018","journal-title":"J. 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