{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:55:23Z","timestamp":1791615323192,"version":"4.3.4"},"reference-count":59,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:00:00Z","timestamp":1672963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>\n                    Cortisol is an essential regulator of neuroendocrine stress responses in teleost. Cortisol performs its effects through the modulation of glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), activating gene expression. Until now the contribution of both receptors in the global transcriptional response in teleost skeletal muscle has not been explored. To understand in a comprehensive and global manner how GR and MR modulates the skeletal muscle transcriptomic response, we performed RNA-seq analysis. Juvenile rainbow trout (\n                    <jats:italic>Oncorhynchus mykiss<\/jats:italic>\n                    ) pretreated with a suppressor of endogenous cortisol production were intraperitoneally injected with cortisol (10\u00a0mg\/kg). We also included a treatment with mifepristone (GR antagonist) and eplerenone (MR antagonist) in the presence or absence of cortisol. cDNA libraries were constructed from the skeletal muscle of rainbow trout groups: vehicle, cortisol, mifepristone, eplerenone, mifepristone\/cortisol and eplerenone\/cortisol. RNA-seq analysis revealed that 135 transcripts were differentially expressed in cortisol vs. mifepristone\/cortisol group, mainly associated to inflammatory response, ion transmembrane transport, and proteolysis. In the other hand, 68 transcripts were differentially expressed in cortisol vs. eplerenone\/cortisol group, mainly associated to muscle contraction, and regulation of cell cycle. To validate these observations, we performed\n                    <jats:italic>in vitro<\/jats:italic>\n                    experiments using rainbow trout myotubes. In myotubes treated with cortisol, we found increased expression of\n                    <jats:italic>cxcr2<\/jats:italic>\n                    ,\n                    <jats:italic>c3<\/jats:italic>\n                    , and\n                    <jats:italic>clca3p<\/jats:italic>\n                    mediated by GR, associated with inflammatory response, proteolysis, and ion transmembrane transport, respectively. Contrastingly, MR modulated the expression of\n                    <jats:italic>myh2<\/jats:italic>\n                    and\n                    <jats:italic>gadd45g<\/jats:italic>\n                    mainly associated with muscle contraction and regulation of cell cycle, respectively. These results suggest that GR and MR have a differential participation in the physiological response to stress in teleost skeletal muscle.\n                  <\/jats:p>","DOI":"10.3389\/fphys.2022.1048008","type":"journal-article","created":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T09:07:22Z","timestamp":1672996042000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["Role of glucocorticoid and mineralocorticoid receptors in rainbow trout (Oncorhynchus mykiss) skeletal muscle: A transcriptomic perspective of cortisol action"],"prefix":"10.3389","volume":"13","author":[{"given":"Jorge E.","family":"Aedo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Zuloaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Aravena-Canales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Molina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Antonio","family":"Vald\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3310","DOI":"10.3390\/ani11113310","article-title":"Differential metabolic and transcriptional responses of gilthead seabream (sparus aurata) administered with cortisol or cortisol-BSA","volume":"11","author":"Aedo","year":"2021","journal-title":"Animals"},{"key":"B2","doi-asserted-by":"publisher","first-page":"100627","DOI":"10.1016\/j.cbd.2019.100627","article-title":"Quantitative proteomics analysis of membrane glucocorticoid receptor activation in rainbow trout skeletal muscle","volume":"32","author":"Aedo","year":"","journal-title":"Comp. 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