{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:26:27Z","timestamp":1762341987033,"version":"3.41.2"},"reference-count":66,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T00:00:00Z","timestamp":1671753600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017995","name":"Nora Eccles Treadwell Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017995","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>Transient receptor potential canonical 1 (TRPC1) channels are Ca<jats:sup>2+<\/jats:sup>-permeable ion channels expressed in cardiomyocytes. An involvement of TRPC1 channels in cardiac diseases is widely established. However, the physiological role of TRPC1 channels and the mechanisms through which they contribute to disease development are still under investigation. Our prior work suggested that TRPC1 forms Ca<jats:sup>2+<\/jats:sup> leak channels located in the sarcoplasmic reticulum (SR) membrane. Prior studies suggested that TRPC1 channels in the cell membrane are mechanosensitive, but this was not yet investigated in cardiomyocytes or for SR localized TRPC1 channels. We applied adenoviral transfection to overexpress or suppress TRPC1 expression in neonatal rat ventricular myocytes (NRVMs). Transfections were evaluated with RT-qPCR, western blot, and fluorescent imaging. Single-molecule localization microscopy revealed high colocalization of exogenously expressed TRPC1 and the sarco\/endoplasmic reticulum Ca<jats:sup>2+<\/jats:sup> ATPase (SERCA2). To test our hypothesis that TRPC1 channels contribute to mechanosensitive Ca<jats:sup>2+<\/jats:sup> SR leak, we directly measured SR Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>SR<\/jats:sub>) using adenoviral transfection with a novel ratiometric genetically encoded SR-targeting Ca<jats:sup>2+<\/jats:sup> sensor. We performed fluorescence imaging to quantitatively assess [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>SR<\/jats:sub> and leak through TRPC1 channels of NRVMs cultured on stretchable silicone membranes. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>SR<\/jats:sub> was increased in cells with suppressed TRPC1 expression vs. control and Transient receptor potential canonical 1-overexpressing cells. We also detected a significant reduction in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>SR<\/jats:sub> in cells with Transient receptor potential canonical 1 overexpression when 10% uniaxial stretch was applied. These findings indicate that TRPC1 channels underlie the mechanosensitive modulation of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>SR<\/jats:sub>. Our findings are critical for understanding the physiological role of TRPC1 channels and support the development of pharmacological therapies for cardiac diseases.<\/jats:p>","DOI":"10.3389\/fphys.2022.1056657","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T07:46:17Z","timestamp":1671781577000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["TRPC1 channels underlie stretch-modulated sarcoplasmic reticulum calcium leak in cardiomyocytes"],"prefix":"10.3389","volume":"13","author":[{"given":"Molly E.","family":"Streiff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea C.","family":"Corbin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azmi A.","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Hunter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank B.","family":"Sachse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,12,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1016\/j.pbiomolbio.2017.06.005","article-title":"Physiological and pathophysiological role of transient receptor potential canonical channels in cardiac myocytes","volume":"130","author":"Ahmad","year":"2017","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"6217","DOI":"10.1523\/JNEUROSCI.0893-09.2009","article-title":"TRPC1 and TRPC6 channels cooperate with TRPV4 to mediate mechanical hyperalgesia and nociceptor sensitization","volume":"29","author":"Alessandri-Haber","year":"2009","journal-title":"J. Neurosci."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1226","DOI":"10.1139\/cjpp-2021-0260","article-title":"Cardiac hypertrophy caused by hyperthyroidism in rats: The role of ATF-6 and TRPC1 channels","volume":"99","author":"Bektur Aykanat","year":"2021","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"36387","DOI":"10.1074\/jbc.M109.073221","article-title":"Transient receptor potential canonical type 1 (TRPC1) operates as a sarcoplasmic reticulum calcium leak channel in skeletal muscle","volume":"284","author":"Berbey","year":"2009","journal-title":"J. Biol. Chem."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.brainres.2011.11.044","article-title":"Critical role of TRPP2 and TRPC1 channels in stretch-induced injury of blood-brain barrier endothelial cells","volume":"1436","author":"Berrout","year":"2012","journal-title":"Brain Res."},{"key":"B7","doi-asserted-by":"publisher","first-page":"27208","DOI":"10.1074\/jbc.M301118200","article-title":"Caveolin-1 contributes to assembly of store-operated Ca2+ influx channels by regulating plasma membrane localization of TRPC1","volume":"278","author":"Brazer","year":"2003","journal-title":"J. Biol. Chem."},{"key":"B8","doi-asserted-by":"publisher","first-page":"696694","DOI":"10.3389\/fphys.2021.696694","article-title":"Need for speed: The importance of physiological strain rates in determining myocardial stiffness","volume":"12","author":"Caporizzo","year":"2021","journal-title":"Front. Physiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1159\/000354498","article-title":"Upregulation of TRPC1 contributes to contractile function in isoproterenol-induced hypertrophic myocardium of rat","volume":"32","author":"Chen","year":"2013","journal-title":"Cell Physiol. Biochem."},{"key":"B10","doi-asserted-by":"publisher","first-page":"e1001025","DOI":"10.1371\/journal.pbio.1001025","article-title":"Local Ca2+ entry via Orai1 regulates plasma membrane recruitment of TRPC1 and controls cytosolic Ca2+ signals required for specific cell functions","volume":"9","author":"Cheng","year":"2011","journal-title":"PLoS Biol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/B978-0-12-407870-3.00007-X","article-title":"Contribution and regulation of TRPC channels in store-operated Ca2+ entry","volume":"71","author":"Cheng","year":"2013","journal-title":"Curr. Top. Membr."},{"key":"B12","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1161\/01.res.67.3.574","article-title":"Developmental changes in cardiac myocyte calcium regulation","volume":"67","author":"Chin","year":"1990","journal-title":"Circ. Res."},{"key":"B13","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/1471-2199-12-30","article-title":"TRPC1 transcript variants, inefficient nonsense-mediated decay and low up-frameshift-1 in vascular smooth muscle cells","volume":"12","author":"Dedman","year":"2011","journal-title":"BMC Mol. Biol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s00424-007-0314-3","article-title":"Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1","volume":"455","author":"Dietrich","year":"2007","journal-title":"Pflugers Arch."},{"key":"B15","doi-asserted-by":"publisher","first-page":"939","DOI":"10.3390\/cells3040939","article-title":"Classical transient receptor potential 1 (TRPC1): Channel or channel regulator?","volume":"3","author":"Dietrich","year":"2014","journal-title":"Cells"},{"key":"B16","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1161\/CIRCRESAHA.110.225888","article-title":"TRPC channels as effectors of cardiac hypertrophy","volume":"108","author":"Eder","year":"2011","journal-title":"Circ. Res."},{"key":"B17","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1007\/978-3-319-57732-6_25","article-title":"Cardiac remodeling and disease: SOCE and TRPC signaling in cardiac pathology","volume":"993","author":"Eder","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"159","DOI":"10.3389\/fphys.2019.00159","article-title":"TRP channels: current perspectives in the adverse cardiac remodeling","volume":"10","author":"Falc\u00f3n","year":"2019","journal-title":"Front. Physiol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"173","DOI":"10.3390\/cells9010173","article-title":"TRPC channels: dysregulation and Ca(2+) mishandling in ischemic heart disease","volume":"9","author":"Falc\u00f3n","year":"2020","journal-title":"Cells"},{"key":"B20","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1152\/jn.00658.2011","article-title":"TRPC1 contributes to light-touch sensation and mechanical responses in low-threshold cutaneous sensory neurons","volume":"107","author":"Garrison","year":"2012","journal-title":"J. Neurophysiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1097","DOI":"10.1007\/s00424-007-0359-3","article-title":"Revisiting TRPC1 and TRPC6 mechanosensitivity","volume":"455","author":"Gottlieb","year":"2008","journal-title":"Pflugers Arch."},{"key":"B22","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1016\/j.bbadis.2007.02.007","article-title":"Involvement of transient receptor potential proteins in cardiac hypertrophy","volume":"1772","author":"Guinamard","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"B23","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/j.cels.2016.03.010","article-title":"Optogenetic control of calcium oscillation waveform defines NFAT as an integrator of calcium load","volume":"2","author":"Hannanta-Anan","year":"2016","journal-title":"Cell Syst."},{"key":"B24","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.yjmcc.2020.01.008","article-title":"Location and function of transient receptor potential canonical channel 1 in ventricular myocytes","volume":"139","author":"Hu","year":"2020","journal-title":"J. Mol. Cell Cardiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.hlc.2007.07.004","article-title":"Store-operated Ca2+ entry and TRPC expression; possible roles in cardiac pacemaker tissue","volume":"16","author":"Ju","year":"2007","journal-title":"Heart Lung Circ."},{"key":"B26","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1016\/j.bbrc.2013.06.002","article-title":"Sildenafil prevents the up-regulation of transient receptor potential canonical channels in the development of cardiomyocyte hypertrophy","volume":"436","author":"Kiso","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B27","doi-asserted-by":"publisher","first-page":"119020","DOI":"10.1016\/j.bbamcr.2021.119020","article-title":"A comprehensive overview of the complex world of the endo- and sarcoplasmic reticulum Ca(2+)-leak channels","volume":"1868","author":"Lemos","year":"2021","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"B28","doi-asserted-by":"publisher","first-page":"2895","DOI":"10.1073\/pnas.0712288105","article-title":"Functional interactions among Orai1, TRPCs, and STIM1 suggest a STIM-regulated heteromeric Orai\/TRPC model for SOCE\/Icrac channels","volume":"105","author":"Liao","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B29","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1007\/s41048-019-0082-6","article-title":"A genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain in the endoplasmic reticulum","volume":"5","author":"Luo","year":"2019","journal-title":"Biophys. Rep."},{"key":"B30","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1038\/ncb1218","article-title":"TRPC1 forms the stretch-activated cation channel in vertebrate cells","volume":"7","author":"Maroto","year":"2005","journal-title":"Nat. Cell Biol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"3092","DOI":"10.1038\/emboj.2008.233","article-title":"Gq-coupled receptors as mechanosensors mediating myogenic vasoconstriction","volume":"27","author":"Mederos y Schnitzler","year":"2008","journal-title":"Embo J."},{"key":"B32","doi-asserted-by":"publisher","first-page":"eaan0724","DOI":"10.1126\/scitranslmed.aan0724","article-title":"Sarcoplasmic reticulum calcium leak contributes to arrhythmia but not to heart failure progression","volume":"10","author":"Mohamed","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"B33","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1016\/j.carpath.2016.08.004","article-title":"Endoglin selectively modulates transient receptor potential channel expression in left and right heart failure","volume":"25","author":"Morine","year":"2016","journal-title":"Cardiovasc Pathol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"jcs238360","DOI":"10.1242\/jcs.238360","article-title":"Mammalian TRP ion channels are insensitive to membrane stretch","volume":"132","author":"Nikolaev","year":"2019","journal-title":"J. Cell Sci."},{"key":"B35","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1007\/s00210-008-0321-8","article-title":"Roles of TRP channels in the development of cardiac hypertrophy","volume":"378","author":"Nishida","year":"2008","journal-title":"Naunyn Schmiedeb. Arch. Pharmacol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1016\/j.yjmcc.2006.10.020","article-title":"Upregulation of TRPC1 in the development of cardiac hypertrophy","volume":"42","author":"Ohba","year":"2007","journal-title":"J. Mol. Cell Cardiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"9105","DOI":"10.1074\/jbc.M608942200","article-title":"Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex is involved in store-operated calcium influx. Evidence for similarities in store-operated and calcium release-activated calcium channel components","volume":"282","author":"Ong","year":"2007","journal-title":"J. Biol. Chem."},{"key":"B38","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1007\/978-3-319-26974-0_5","article-title":"Role of TRPC channels in store-operated calcium entry","volume":"898","author":"Ong","year":"2016","journal-title":"Adv. Exp. Med. Biol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1152\/physrev.00057.2003","article-title":"Store-operated calcium channels","volume":"85","author":"Parekh","year":"2005","journal-title":"Physiol. Rev."},{"key":"B40","doi-asserted-by":"publisher","first-page":"ra46","DOI":"10.1126\/scisignal.2000769","article-title":"beta-Arrestin-biased agonism of the angiotensin receptor induced by mechanical stress","volume":"3","author":"Rakesh","year":"2010","journal-title":"Sci. Signal"},{"key":"B41","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1007\/s12265-010-9208-4","article-title":"TRP-ing up heart and vessels: canonical transient receptor potential channels and cardiovascular disease","volume":"3","author":"Rowell","year":"2010","journal-title":"J. Cardiovasc Transl. Res."},{"key":"B42","doi-asserted-by":"publisher","first-page":"30530","DOI":"10.1074\/jbc.M115.682583","article-title":"Store-operated Ca2+ entry mediated by Orai1 and TRPC1 participates to insulin secretion in rat \u03b2-cells","volume":"290","author":"Sabourin","year":"2015","journal-title":"J. Biol. Chem."},{"key":"B43","doi-asserted-by":"publisher","first-page":"13394","DOI":"10.1074\/jbc.M115.693911","article-title":"Transient receptor potential canonical (TRPC)\/Orai1-dependent store-operated Ca2+ channels: new targets of aldosterone in cardiomyocytes","volume":"291","author":"Sabourin","year":"2016","journal-title":"J. Biol. Chem."},{"key":"B44","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1113\/jphysiol.2006.119305","article-title":"Angiotensin II activates two cation conductances with distinct TRPC1 and TRPC6 channel properties in rabbit mesenteric artery myocytes","volume":"577","author":"Saleh","year":"2006","journal-title":"J. Physiol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"2463","DOI":"10.1113\/jphysiol.2008.152157","article-title":"Diverse properties of store-operated TRPC channels activated by protein kinase C in vascular myocytes","volume":"586","author":"Saleh","year":"2008","journal-title":"J. Physiol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"2111","DOI":"10.1016\/j.bpj.2010.01.042","article-title":"Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes","volume":"98","author":"Santiago","year":"2010","journal-title":"Biophys. J."},{"key":"B47","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.1161\/CIRCRESAHA.109.206581","article-title":"TRPC1 channels are critical for hypertrophic signaling in the heart","volume":"105","author":"Seth","year":"2009","journal-title":"Circ. Res."},{"key":"B48","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1161\/01.res.0000036914.12686.28","article-title":"Quantitative assessment of the SR Ca2+ leak-load relationship","volume":"91","author":"Shannon","year":"2002","journal-title":"Circ. Res."},{"key":"B49","doi-asserted-by":"publisher","first-page":"e009291","DOI":"10.1161\/CIRCEP.120.009291","article-title":"Activation of TRPC (transient receptor potential canonical) channel currents in iron overloaded cardiac myocytes","volume":"14","author":"Siri-Angkul","year":"2021","journal-title":"Circ. Arrhythm. Electrophysiol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"1772","DOI":"10.1016\/j.bbamcr.2015.04.016","article-title":"Functional and physiopathological implications of TRP channels","volume":"1853","author":"Smani","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"B51","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.neulet.2009.03.082","article-title":"Down regulation of TRPC1 by shRNA reduces mechanosensitivity in mouse dorsal root ganglion neurons in vitro","volume":"457","author":"Staaf","year":"2009","journal-title":"Neurosci. Lett."},{"key":"B52","doi-asserted-by":"publisher","first-page":"H1241","DOI":"10.1152\/ajpheart.00818.2011","article-title":"G protein-mediated stretch reception","volume":"302","author":"Storch","year":"2012","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"B53","doi-asserted-by":"publisher","first-page":"916278","DOI":"10.3389\/fphys.2022.916278","article-title":"Effects of sarcolemmal background Ca(2+) entry and sarcoplasmic Ca(2+) leak currents on electrophysiology and Ca(2+) transients in human ventricular cardiomyocytes: A computational comparison","volume":"13","author":"Streiff","year":"2022","journal-title":"Front. Physiol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"4153","DOI":"10.1038\/ncomms5153","article-title":"Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA","volume":"5","author":"Suzuki","year":"2014","journal-title":"Nat. Commun."},{"key":"B55","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/j.yjmcc.2018.10.020","article-title":"Inhibition of TRPC1 prevents cardiac hypertrophy via NF-\u03baB signaling pathway in human pluripotent stem cell-derived cardiomyocytes","volume":"126","author":"Tang","year":"2019","journal-title":"J. Mol. Cell Cardiol."},{"key":"B56","doi-asserted-by":"publisher","first-page":"382586","DOI":"10.1155\/2011\/382586","article-title":"Dynamic changes in sarcoplasmic reticulum structure in ventricular myocytes","volume":"2011","author":"Vega","year":"2011","journal-title":"J. Biomed. Biotechnol."},{"key":"B57","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.cophys.2017.12.001","article-title":"Recent insights on the role of TRP channels in cardiac muscle","volume":"1","author":"Vennekens","year":"2018","journal-title":"Curr. Opin. Physiology"},{"key":"B58","doi-asserted-by":"publisher","first-page":"e58388","DOI":"10.7554\/eLife.58388","article-title":"The push-to-open mechanism of the tethered mechanosensitive ion channel NompC","volume":"10","author":"Wang","year":"2021","journal-title":"Elife"},{"key":"B59","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.pharmthera.2008.03.008","article-title":"TRP channel and cardiovascular disease","volume":"118","author":"Watanabe","year":"2008","journal-title":"Pharmacol. Ther."},{"key":"B60","doi-asserted-by":"publisher","first-page":"1785","DOI":"10.3389\/fphys.2018.01785","article-title":"Potential arrhythmogenic role of TRPC channels and store-operated calcium entry mechanism in mouse ventricular myocytes","volume":"9","author":"Wen","year":"2018","journal-title":"Front. Physiol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"3685","DOI":"10.1093\/eurheartj\/ehz326","article-title":"Modelling diastolic dysfunction in induced pluripotent stem cell-derived cardiomyocytes from hypertrophic cardiomyopathy patients","volume":"40","author":"Wu","year":"2019","journal-title":"Eur. Heart J."},{"key":"B62","doi-asserted-by":"publisher","first-page":"471","DOI":"10.4062\/biomolther.2016.096","article-title":"Canonical transient receptor potential channels and their link with cardio\/cerebro-vascular diseases","volume":"25","author":"Xiao","year":"2017","journal-title":"Biomol. Ther. Seoul."},{"key":"B63","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.jbiomech.2016.12.002","article-title":"Effects of transient receptor potential canonical 1 (TRPC1) on the mechanical stretch-induced expression of airway remodeling-associated factors in human bronchial epithelioid cells","volume":"51","author":"Yu","year":"2017","journal-title":"J. Biomech."},{"key":"B64","doi-asserted-by":"publisher","first-page":"H157","DOI":"10.1152\/ajpheart.00457.2014","article-title":"Role of TRP channels in the cardiovascular system","volume":"308","author":"Yue","year":"2015","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"B65","doi-asserted-by":"publisher","first-page":"1391","DOI":"10.1016\/j.cell.2015.08.024","article-title":"Ankyrin repeats convey force to gate the NOMPC mechanotransduction channel","volume":"162","author":"Zhang","year":"2015","journal-title":"Cell"},{"key":"B66","doi-asserted-by":"publisher","first-page":"4743","DOI":"10.1113\/jphysiol.2010.197913","article-title":"Ca2+ spark-dependent and -independent sarcoplasmic reticulum Ca2+ leak in normal and failing rabbit ventricular myocytes","volume":"588","author":"Zima","year":"2010","journal-title":"J. Physiol."},{"key":"B67","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1038\/ncb1137","article-title":"Mechanical stress activates angiotensin II type 1 receptor without the involvement of angiotensin II","volume":"6","author":"Zou","year":"2004","journal-title":"Nat. Cell Biol."}],"container-title":["Frontiers in Physiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphys.2022.1056657\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T07:46:19Z","timestamp":1671781579000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphys.2022.1056657\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,23]]},"references-count":66,"alternative-id":["10.3389\/fphys.2022.1056657"],"URL":"https:\/\/doi.org\/10.3389\/fphys.2022.1056657","relation":{},"ISSN":["1664-042X"],"issn-type":[{"type":"electronic","value":"1664-042X"}],"subject":[],"published":{"date-parts":[[2022,12,23]]},"article-number":"1056657"}}