{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T11:31:02Z","timestamp":1791804662020,"version":"4.3.4"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE027323, R01DE026730, U24DE029463"],"award-info":[{"award-number":["R01DE027323, R01DE026730, U24DE029463"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>As a basic science, craniofacial research embraces multiple facets spanning from molecular regulation of craniofacial development, cell biology\/signaling and ultimately translational craniofacial biology. Calvarial sutures coordinate development of the skull, and the premature fusion of one or more, leads to craniosynostosis. Animal models provide significant contributions toward craniofacial biology and clinical\/surgical treatments of patients with craniofacial disorders. Studies employing mouse models are costly and time consuming for housing\/breeding. Herein, we present the establishment of a calvarial suture explant 2-D culture method that has been proven to be a reliable system showing fidelity with the <jats:italic>in vivo<\/jats:italic> harvesting procedure to isolate high yields of skeletal stem\/progenitor cells from small number of mice. Moreover, this method allows the opportunity to phenocopying models of craniosynostosis and <jats:italic>in vitro<\/jats:italic> tamoxifen-induction of <jats:italic>Actin<jats:sup>creERT2<\/jats:sup>;R26<jats:sup>Rainbow<\/jats:sup><\/jats:italic> suture explants to trace clonal expansion. This versatile method tackles needs of large number of mice to perform calvarial suture research.<\/jats:p>","DOI":"10.3389\/fphys.2022.823661","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T10:17:40Z","timestamp":1644488260000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Harnessing a Feasible and Versatile ex vivo Calvarial Suture 2-D Culture System to Study Suture Biology"],"prefix":"10.3389","volume":"13","author":[{"given":"Natalina","family":"Quarto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddharth","family":"Menon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Griffin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julika","family":"Huber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"Longaker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"W537","DOI":"10.1093\/nar\/gky379","article-title":"The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update.","volume":"46","author":"Afgan","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"H234","DOI":"10.1152\/ajpheart.00227.2012","article-title":"Effects of the absence of procollagen C-endopeptidase enhancer-2 on myocardial collagen accumulation in chronic pressure overload.","volume":"303","author":"Baicu","year":"2012","journal-title":"Am. 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