{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:59:01Z","timestamp":1791874741785,"version":"4.3.4"},"reference-count":71,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T00:00:00Z","timestamp":1687219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>The distribution of octopuses within the <jats:italic>Octopus vulgaris<\/jats:italic> species complex remains inadequately understood. Species determination can be complex and involves characterizing a specimen\u2019s physical features and comparing its genetic makeup to other populations. In this study, we present the first genetic confirmation of <jats:italic>Octopus insularis<\/jats:italic> (Leite and Haimovici, 2008) inhabiting the coastal waters of the Florida Keys, United States. We employed visual observations to identify species-specific body patterns of three wild-caught octopuses and used <jats:italic>de novo<\/jats:italic> genome assembly to confirm their species. All three specimens exhibited a red\/white reticulated pattern on their ventral arm surface. Two specimens displayed body pattern components of deimatic display (white eye encircled by a light ring, with darkening around the eye). All visual observations were consistent with distinguishing features of <jats:italic>O. insularis<\/jats:italic>. We then compared mitochondrial subunits COI, COIII, and 16S in these specimens across all available annotated octopod sequences, including <jats:italic>Sepia apama<\/jats:italic> (<jats:xref>Hotaling et al., 2021<\/jats:xref>) as a control outgroup taxon. For species exhibiting intraspecific genomic variation, we included multiple sequences from geographically distinct populations. Laboratory specimens consistently clustered into a single taxonomic node with <jats:italic>O. insularis<\/jats:italic>. These findings confirm <jats:italic>O. insularis<\/jats:italic> presence in South Florida and suggest a more extensive northern distribution than previously assumed. Whole genome Illumina sequencing of multiple specimens enabled taxonomic identification with well-established DNA barcodes while also generating the first <jats:italic>de novo<\/jats:italic> full assembly of <jats:italic>O. insularis<\/jats:italic>. Furthermore, constructing and comparing phylogenetic trees for multiple conserved genes is essential for confirming the presence and delineation of cryptic species in the Caribbean.<\/jats:p>","DOI":"10.3389\/fphys.2023.1162807","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T05:22:53Z","timestamp":1687238573000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Genetic confirmation of Octopus insularis (Leite and Haimovici, 2008) in South Florida, United States using physical features and de novo genome assembly"],"prefix":"10.3389","volume":"14","author":[{"given":"Brigid","family":"Maloney","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric Angel","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chelsea O.","family":"Bennice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo O.","family":"Magnasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,6,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"W537","DOI":"10.1093\/nar\/gky379","article-title":"The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update","volume":"46","author":"Afgan","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"B2","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1038\/nature14668","article-title":"The octopus genome and the evolution of cephalopod neural and morphological novelties","volume":"524","author":"Albertin","year":"2015","journal-title":"Nature"},{"key":"B3","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.dsr2.2010.05.016","article-title":"Cryptic speciation and the circumpolarity debate: A case study on endemic southern ocean octopuses using the COI barcode of life","volume":"58","author":"Allcock","year":"2011","journal-title":"Deep Sea Res. 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