{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T17:50:00Z","timestamp":1784224200600,"version":"3.55.0"},"reference-count":23,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T00:00:00Z","timestamp":1694563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/00191-4"],"award-info":[{"award-number":["2018\/00191-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p><jats:bold>Introduction:<\/jats:bold> The medial preoptic area (mPOA) participates in thermoregulatory control and blood pressure modulation as shown by studies with electrical stimulation of this area or cobalt chloride injection, a non-selective synapse inhibitor. This study aimed to investigate whether angiotensin II (Ang II) and GABA could act or not in the mPOA to mediate the cardiovascular and micturition control pathways.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Female Wistar rats were submitted to stereotaxic surgery for implantation of a guide cannula into the mPOA 7\u00a0days prior to the experiments. Afterwards, the animals were isoflurane- anesthetized and submitted to the catheterization of the femoral artery and vein and urinary bladder cannulation for mean arterial pressure (MAP), heart rate (HR), and intravesical pressure (IP) recordings, respectively. After the baseline MAP, HR, and IP recordings for 15\u00a0min, Ang II (0.1\u00a0nM, 1\u00a0\u03bcL), losartan (AT-1 receptor antagonist, 100\u00a0nM, 1\u00a0\u03bcL), GABA (50\u00a0mM, 1\u00a0\u03bcL) or saline (1\u00a0\u03bcL) were injected into the mPOA, and the variables were measured for additional 30\u00a0min. In a different group of rats, the AT-1 receptor, angiotensin II converting enzyme (ACE), and GABAa receptor gene expression was evaluated in mPOA samples by qPCR. The data are as mean \u00b1 SEM and submitted to One-way ANOVA (Tukey posttest) or paired Student t-test (P &amp;lt;0.05).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The injection of Ang II into the mPOA evoked a significant hypotension (\u221237\u00b110\u00a0mmHg, n = 6, <jats:italic>p<\/jats:italic> = 0.024) and bradycardia (\u221247 \u00b1 20\u00a0bpm, <jats:italic>p<\/jats:italic> = 0.030) compared to saline (+1 \u00b1 1\u00a0mmHg and +6 \u00b1 2\u00a0bpm, n = 6). A significant increase in IP was observed after Ang II injection into the mPOA (+72.25 \u00b1 17.91%, <jats:italic>p<\/jats:italic> = 0.015 vs. \u22121.80 \u00b1 2.98%, n = 6, saline). No significant changes were observed in MAP, HR and IP after the losartan injection in the mPOA compared to saline injection. Injection of GABA into the mPOA evoked a significant fall in MAP and HR (\u221268 \u00b1 2\u00a0mmHg, n = 6, <jats:italic>p<\/jats:italic> &amp;lt; 0.0001 and \u2212115 \u00b1 14\u00a0bpm, n = 6, <jats:italic>p<\/jats:italic> = 0.0002 vs. \u22121 \u00b1 1\u00a0mmHg and +4 \u00b1 2\u00a0bpm, n = 6, saline), but no significant changes were observed in IP. The AT-1 receptor, ACE and GABAa receptor mRNA expression was observed in all mPOA samples.<\/jats:p><jats:p><jats:bold>Discussion:<\/jats:bold> Therefore, in female rats, Ang II mediated transmission in the mPOA is involved in the cardiovascular regulation and in the control of central micturition pathways. A phasic control dependent on AT-1 receptors in the mPOA seems to be involved in the regulation of those cardiovascular and intravesical 3 parameters. In contrast, GABAergic transmission in the mPOA participates in the pathways of cardiovascular control in anesthetized female rats, nevertheless, this neurotransmission is not involved in the micturition control.<\/jats:p>","DOI":"10.3389\/fphys.2023.1224505","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T12:31:28Z","timestamp":1694608288000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Angiotensinergic and GABAergic transmission in the medial preoptic area: role in urinary bladder and cardiovascular control in female rats"],"prefix":"10.3389","volume":"14","author":[{"given":"Sergio A.","family":"Daiuto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo P.","family":"de Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e1rbara do","family":"Vale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuha A.","family":"Dsouki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele","family":"Giannocco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo M.","family":"Cafarchio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrik","family":"Aronsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica A.","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,9,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"17097","DOI":"10.1038\/nrdp.2017.97","article-title":"Urinary incontinence in women","volume":"3","author":"Aoki","year":"2017","journal-title":"Nat. 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