{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:24:50Z","timestamp":1787027090973,"version":"build-2736575974"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T00:00:00Z","timestamp":1709769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>\n                    The genetic male sterile line (\n                    <jats:italic>GMS<\/jats:italic>\n                    ) of the silkworm\n                    <jats:italic>Bombyx mori<\/jats:italic>\n                    is a recessive mutant that is naturally mutated from the wild-type\n                    <jats:italic>898WB<\/jats:italic>\n                    strain. One of the major characteristics of the\n                    <jats:italic>GMS<\/jats:italic>\n                    mutant is its small larvae. Through positional cloning, candidate genes for the\n                    <jats:italic>GMS<\/jats:italic>\n                    mutant were located in a region approximately 800.5\u00a0kb long on the 24th linkage group of the silkworm. One of the genes was\n                    <jats:italic>Bombyx mori<\/jats:italic>\n                    CCAAT\/enhancer-binding protein zeta (\n                    <jats:italic>BmC\/EBPZ<\/jats:italic>\n                    ), which is a member of the basic region-leucine zipper transcription factor family. Compared with the wild-type\n                    <jats:italic>898WB<\/jats:italic>\n                    strain, the\n                    <jats:italic>GMS<\/jats:italic>\n                    mutant features a 9\u00a0bp insertion in the 3\u2032end of open reading frame sequence of\n                    <jats:italic>BmC\/EBPZ<\/jats:italic>\n                    gene. Moreover, the high expression level of the\n                    <jats:italic>BmC\/EBPZ<\/jats:italic>\n                    gene in the testis suggests that the gene is involved in the regulation of reproduction-related genes. Using the CRISPR\/Cas9-mediated knockout system, we found that the\n                    <jats:italic>BmC\/EBPZ<\/jats:italic>\n                    knockout strains had the same phenotypes as the\n                    <jats:italic>GMS<\/jats:italic>\n                    mutant, that is, the larvae were small. However, the larvae of\n                    <jats:italic>BmC\/EBPZ<\/jats:italic>\n                    knockout strains died during the development of the third instar. Therefore, the\n                    <jats:italic>BmC\/EBPZ<\/jats:italic>\n                    gene was identified as the major gene responsible for\n                    <jats:italic>GMS<\/jats:italic>\n                    mutation.\n                  <\/jats:p>","DOI":"10.3389\/fphys.2024.1298869","type":"journal-article","created":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T09:51:46Z","timestamp":1709805106000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["BmC\/EBPZ gene is essential for the larval growth and development of silkworm, Bombyx mori"],"prefix":"10.3389","volume":"15","author":[{"given":"Xinglin","family":"Mei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianchen","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anli","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongxu","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peitong","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoling","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,3,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1002\/j.1460-2075.1990.tb08316.x","article-title":"A nuclear factor for IL-6 expression (NF-IL6) is a member of a C\/EBP family","volume":"9","author":"Akira","year":"1990","journal-title":"EMBO J."},{"key":"B2","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1186\/1471-2164-7-94","article-title":"Bioinformatic screening of human ESTs for differentially expressed genes in normal and tumor tissues","volume":"7","author":"Aouacheria","year":"2006","journal-title":"BMC genomics"},{"key":"B3","doi-asserted-by":"publisher","first-page":"917","DOI":"10.7150\/ijbs.7224","article-title":"The many faces of C\/EBP\u03b4 and their relevance for inflammation and cancer","volume":"9","author":"Balamurugan","year":"2013","journal-title":"Int. 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