{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T06:10:03Z","timestamp":1756793403460,"version":"3.44.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>Astronauts endure repetitive episodes of hypergravity (HG) during takeoff and landing of spaceflights, and also in space due to microgravity. Blood-brain barrier alterations and disruptions were recently reported in mice subjected to a short period of HG induced by centrifugation. In this study, we have evaluated if the blood-spinal cord barrier (BSCB) was also damaged by centrifugation-induced HG in mice. For that purpose, adult C57Bl\/6J male mice were subjected to HG in a centrifuge at 2 g for 24 h, while control mice stayed in normogravity (n = 7-8 per group). Mice were sacrificed after centrifugation, and thoracic spinal cords collected for immunohistochemistry. Alterations of the BSCB were evaluated by measuring the immunoreactive areas of tight junction-associated proteins (claudin-5, occludin and zonula occludens-1 (ZO-1)) normalized to that of collagen IV-positive vessels. Additionally, the extravasation of a large blood-derived protein, fibrinogen, was quantified to determine if BSCB integrity was strongly impaired. Interestingly, a significant decrease in occludin level was measured in the spinal cord of HG 2 g mice compared to that of control 1 g mice (\u221228.6%, p = 0.0378), whereas claudin-5 (\u221220.6%, p = 0.2030) and ZO-1 (\u221219.6%, p = 0.3048) levels were not significantly affected. The decrease in occludin tight junction proteins was not accompanied by an extravasation of fibrinogen into the spinal cord parenchyma (p &amp;gt; 0.05). Overall, this study reports for the first time structural alterations of the BSCB in the context of hypergravity.<\/jats:p>","DOI":"10.3389\/fphys.2025.1658389","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T05:32:10Z","timestamp":1756791130000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Blood-spinal cord barrier alterations in a mouse model of centrifugation-induced hypergravity"],"prefix":"10.3389","volume":"16","author":[{"given":"David","family":"Dubayle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Luc","family":"Morel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sighild","family":"Lemarchant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"593026","DOI":"10.3389\/fphys.2020.593026","article-title":"Blood-brain barrier leakage is increased in parkinson's disease","volume":"11","author":"Al-Bachari","year":"2020","journal-title":"Front. 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