{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:07:31Z","timestamp":1781104051495,"version":"3.54.1"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL145875"],"award-info":[{"award-number":["HL145875"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Chemical ablation of renal sensory nerves using agonists for transient receptor potential vanilloid-1 (TRPV1) lowers arterial blood pressure (ABP) in multiple experimental models of hypertension. Interestingly, both afferent renal nerve activity and arterial blood pressure were significantly attenuated in male Trpv1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      rats after 2-kidney-1-clip (2K1C) renovascular hypertension. However, TRPV1 expression in sensory neurons differs across species and is lower in mice versus rats or humans. Therefore, the current study assessed the proportion of TRPV1 in mouse renal sensory neurons and tested whether deletion of TRPV1 altered renovascular hypertension in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      2K1C surgery was performed by placement of a 0.5mm length of polyetrafluoroethylene tubing around the right renal artery. Experiment 1 quantified the proportion of TRPV1 mouse renal sensory neurons in both sham and 2K1C after a kidney injection of the tracer wheat germ agglutinin conjugated to AlexaFluor 647. Experiment 2 assessed ABP using telemetry in WT and Trpv1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice after 2K1C.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      First, the majority of retrogradely labeled neurons were located in the ipsilateral T10-L2 dorsal root ganglion and small to medium sized (10-29um diameter). Approximately 60% were TRPV1-positive. Second, 2K1C significantly increased ABP in both male and female WT and Trpv1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice. However, the magnitude of the hypertension was not statistically different between strain and sex. Depressor responses to ganglionic blockade also did not differ between strains and sex.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings suggest that a subset of renal sensory neurons in the mouse are TRPV1-positive, and renovascular 2K1C hypertension is not attenuated in the Trpv1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mouse.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphys.2025.1713864","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T05:15:38Z","timestamp":1764306938000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["2-Kidney-1-clip hypertension is not attenuated in mice lacking the transient receptor potential vanilloid type 1 (TRPV1) channel"],"prefix":"10.3389","volume":"16","author":[{"given":"Sean D.","family":"Stocker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Benoit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob B.","family":"Sullivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline B.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,11,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.1161\/HYPERTENSIONAHA.116.07850","article-title":"Resting afferent renal nerve discharge and renal inflammation: elucidating the role of afferent and efferent renal nerves in deoxycorticosterone acetate salt hypertension","volume":"68","author":"Banek","year":"2016","journal-title":"Hypertension"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1811","DOI":"10.1161\/HYPERTENSIONAHA.123.22620","article-title":"IL-1R mediated activation of renal sensory nerves in DOCA-Salt hypertension","volume":"81","author":"Baumann","year":"2024","journal-title":"Hypertension"},{"key":"B3","doi-asserted-by":"publisher","first-page":"eadj9173","DOI":"10.1126\/sciadv.adj9173","article-title":"Harmonized cross-species cell atlases of trigeminal and dorsal root ganglia","volume":"10","author":"Bhuiyan","year":"2024","journal-title":"Sci. Adv."},{"key":"B4","doi-asserted-by":"publisher","first-page":"878","DOI":"10.1161\/01.hyp.25.4.878","article-title":"Renal afferent denervation prevents hypertension in rats with chronic renal failure","volume":"25","author":"Campese","year":"1995","journal-title":"Hypertension"},{"key":"B5","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1126\/science.288.5464.306","article-title":"Impaired nociception and pain sensation in mice lacking the capsaicin receptor","volume":"288","author":"Caterina","year":"2000","journal-title":"Science"},{"key":"B6","doi-asserted-by":"publisher","first-page":"10119","DOI":"10.1523\/JNEUROSCI.1299-11.2011","article-title":"Restriction of transient receptor potential vanilloid-1 to the peptidergic subset of primary afferent neurons follows its developmental downregulation in nonpeptidergic neurons","volume":"31","author":"Cavanaugh","year":"2011","journal-title":"J. Neurosci."},{"key":"B7","doi-asserted-by":"publisher","first-page":"1627","DOI":"10.1113\/JP271198","article-title":"Dopamine modulation of transient receptor potential vanilloid type 1 (TRPV1) receptor in dorsal root ganglia neurons","volume":"594","author":"Chakraborty","year":"2016","journal-title":"J. Physiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"2639","DOI":"10.1113\/JP273455","article-title":"Presynaptic inhibition of transient receptor potential vanilloid type 1 (TRPV1) receptors by noradrenaline in nociceptive neurons","volume":"595","author":"Chakraborty","year":"2017","journal-title":"J. Physiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/s12933-015-0183-6","article-title":"Activation of TRPV1 channel by dietary capsaicin improves visceral fat remodeling through connexin43-mediated Ca2+ influx","volume":"14","author":"Chen","year":"2015","journal-title":"Cardiovasc Diabetol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"H117","DOI":"10.1152\/ajpheart.00675.2020","article-title":"Impact of anesthesia, sex, and circadian cycle on renal afferent nerve sensitivity","volume":"320","author":"DeLalio","year":"2021","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1002\/cne.902740103","article-title":"Ultrastructural localization and characterization of sensory nerves in the rat kidney","volume":"274","author":"Ferguson","year":"1988","journal-title":"J. Comp. Neurol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1007\/BF00219744","article-title":"The innervation of the renal cortex in the dog. An ultrastructural study","volume":"253","author":"Ferguson","year":"1988","journal-title":"Cell Tissue Res."},{"key":"B13","doi-asserted-by":"publisher","first-page":"R112","DOI":"10.1152\/ajpregu.00427.2014","article-title":"A novel method of selective ablation of afferent renal nerves by periaxonal application of capsaicin","volume":"308","author":"Foss","year":"2015","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1016\/j.neuron.2016.11.007","article-title":"SHANK3 deficiency impairs heat Hyperalgesia and TRPV1 signaling in primary sensory neurons","volume":"92","author":"Han","year":"2016","journal-title":"Neuron"},{"key":"B15","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1146\/annurev-cellbio-101011-155833","article-title":"TRP channels and pain","volume":"29","author":"Julius","year":"2013","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.rvsc.2016.06.002","article-title":"Development of renal atrophy in murine 2 kidney 1 clip hypertension is strain independent","volume":"107","author":"Kashyap","year":"2016","journal-title":"Res. Vet. Sci."},{"key":"B17","doi-asserted-by":"publisher","first-page":"I149","DOI":"10.1161\/01.hyp.5.2_pt_2.i149","article-title":"Hemodynamic and afferent renal nerve responses to intrarenal adenosine in the dog","volume":"5","author":"Katholi","year":"1983","journal-title":"Hypertension"},{"key":"B18","doi-asserted-by":"publisher","first-page":"28621","DOI":"10.1038\/srep28621","article-title":"Systemic desensitization through TRPA1 channels by capsazepine and mustard oil - a novel strategy against inflammation and pain","volume":"6","author":"Kistner","year":"2016","journal-title":"Sci. Rep."},{"key":"B19","doi-asserted-by":"publisher","first-page":"R79","DOI":"10.1152\/ajpregu.00351.2014","article-title":"Role of renal sensory nerves in physiological and pathophysiological conditions","volume":"308","author":"Kopp","year":"2015","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"R411","DOI":"10.1152\/ajpregu.00072.2023","article-title":"Renal and hypothalamic inflammation in renovascular hypertension: role of afferent renal nerves","volume":"325","author":"Lauar","year":"2023","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1016\/j.neuroscience.2005.12.020","article-title":"The role of the capsaicin receptor TRPV1 and acid-sensing ion channels (ASICS) in proton sensitivity of subpopulations of primary nociceptive neurons in rats and mice","volume":"139","author":"Leffler","year":"2006","journal-title":"Neuroscience"},{"key":"B22","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1007\/s00424-019-02346-4","article-title":"Afferent innervation of the ischemic kidney contributes to renal dysfunction in renovascular hypertensive rats","volume":"472","author":"Lopes","year":"2020","journal-title":"Pflugers Arch."},{"key":"B23","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1002\/cne.903110309","article-title":"Sensory innervation of the rat kidney and ureter as revealed by the anterograde transport of wheat germ agglutinin-horseradish peroxidase (WGA-HRP) from dorsal root ganglia","volume":"311","author":"Marfurt","year":"1991","journal-title":"J. Comp. Neurol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1016\/j.bbadis.2007.02.002","article-title":"TRP channels in disease","volume":"1772","author":"Nilius","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"B25","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1152\/jn.00173.2019","article-title":"Renal sensory nerves increase sympathetic nerve activity and blood pressure in 2-kidney 1-clip hypertensive mice","volume":"122","author":"Ong","year":"2019","journal-title":"J. Neurophysiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1002\/cphy.c150047","article-title":"Renal nerves and long-term control of arterial pressure","volume":"7","author":"Osborn","year":"2017","journal-title":"Compr. Physiol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1146\/annurev-physiol-031620-091656","article-title":"Function of renal nerves in kidney physiology and pathophysiology","volume":"83","author":"Osborn","year":"2021","journal-title":"Annu. Rev. Physiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1161\/01.res.43.4.534","article-title":"Renal chemoreceptors in the rat","volume":"43","author":"Recordati","year":"1978","journal-title":"Circ. Res."},{"key":"B29","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1161\/01.res.46.3.395","article-title":"Renal receptors in the rat sensitive to chemical alterations of their environment","volume":"46","author":"Recordati","year":"1980","journal-title":"Circ. Res."},{"key":"B30","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/0165-1838(81)90066-7","article-title":"Renal chemoreceptors","volume":"3","author":"Recordati","year":"1981","journal-title":"J. Auton. Nerv. Syst."},{"key":"B31","doi-asserted-by":"publisher","first-page":"3171","DOI":"10.1111\/j.1460-9568.2008.06267.x","article-title":"Neurokinin 2 receptor-mediated activation of protein kinase C modulates capsaicin responses in DRG neurons from adult rats","volume":"27","author":"Sculptoreanu","year":"2008","journal-title":"Eur. J. Neurosci."},{"key":"B32","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1007\/BF01188046","article-title":"Selective neuronal glycoconjugate expression in sensory and autonomic ganglia: relation of lectin reactivity to peptide and enzyme markers","volume":"19","author":"Silverman","year":"1990","journal-title":"J. Neurocytol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1671","DOI":"10.1161\/HYPERTENSIONAHA.123.21153","article-title":"Deletion of the transient receptor potential Vanilloid 1 channel attenuates sympathoexcitation and hypertension and improves glomerular filtration rate in 2-Kidney-1-Clip rats","volume":"80","author":"Stocker","year":"2023","journal-title":"Hypertension"},{"key":"B34","doi-asserted-by":"publisher","first-page":"6497","DOI":"10.1523\/JNEUROSCI.19-15-06497.1999","article-title":"Isolectin B(4)-positive and -negative nociceptors are functionally distinct","volume":"19","author":"Stucky","year":"1999","journal-title":"J. Neurosci."},{"key":"B35","doi-asserted-by":"publisher","first-page":"R1285","DOI":"10.1152\/ajpregu.00003.2008","article-title":"Mice lacking the transient receptor vanilloid potential 1 channel display normal thirst responses and central Fos activation to hypernatremia","volume":"294","author":"Taylor","year":"2008","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"974197","DOI":"10.3389\/fnins.2023.974197","article-title":"Periglomerular afferent innervation of the mouse renal cortex","volume":"17","author":"Tyshynsky","year":"2023","journal-title":"Front. Neurosci."},{"key":"B37","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1161\/HYPERTENSIONAHA.108.110197","article-title":"Deletion of transient receptor potential vanilloid type 1 receptors exaggerates renal damage in deoxycorticosterone acetate-salt hypertension","volume":"52","author":"Wang","year":"2008","journal-title":"Hypertension"},{"key":"B38","doi-asserted-by":"publisher","first-page":"R1634","DOI":"10.1152\/ajpregu.00235.2009","article-title":"P2Y2 receptors mediate ATP-induced resensitization of TRPV1 expressed by kidney projecting sensory neurons","volume":"298","author":"Wang","year":"2010","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"F1455","DOI":"10.1152\/ajprenal.00645.2011","article-title":"Genetic deficiency of Smad3 protects the kidneys from atrophy and interstitial fibrosis in 2K1C hypertension","volume":"302","author":"Warner","year":"2012","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"H82","DOI":"10.1152\/ajpheart.1986.250.1.H82","article-title":"Sensory denervation of the kidney attenuates renovascular hypertension in the rat","volume":"250","author":"Wyss","year":"1986","journal-title":"Am. J. Physiol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"12067","DOI":"10.1523\/JNEUROSCI.0496-07.2007","article-title":"Neurokinin-1 receptor enhances TRPV1 activity in primary sensory neurons via PKCepsilon: a novel pathway for heat hyperalgesia","volume":"27","author":"Zhang","year":"2007","journal-title":"J. Neurosci."}],"container-title":["Frontiers in Physiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphys.2025.1713864\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T05:15:38Z","timestamp":1764306938000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphys.2025.1713864\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,28]]},"references-count":41,"alternative-id":["10.3389\/fphys.2025.1713864"],"URL":"https:\/\/doi.org\/10.3389\/fphys.2025.1713864","relation":{},"ISSN":["1664-042X"],"issn-type":[{"value":"1664-042X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,28]]},"article-number":"1713864"}}