{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T06:19:53Z","timestamp":1773037193048,"version":"3.50.1"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"\n Endogenous mucosal phosphatases of chicken degrade phytate from the feed to a variable extent. The objective of this study was to investigate endogenous mucosal phosphatases as affected by laying hen strain, hen age, and dietary phosphorus (P) renunciation. Two cohorts of the strains Lohmann Brown-classic (LB) and Lohmann LSL-classic (LSL) were fed for 3 weeks, starting in week 27 and 39 of age, respectively, one of two diets with (P+) or without (P\u2212) mineral P supplement. Total excreta were collected. In weeks 30 and 42, hens were sacrificed, and ileal digesta and duodenum mucosa were collected. Mucosa was freeze-dried before being used in a three-step\n in vitro<\/jats:italic>\n assay. The concentrations of InsP isomers,\n myo<\/jats:italic>\n -inositol (MI), P, and calcium (Ca) were measured in the excreta and digesta. InsP isomers were measured in the\n in vitro<\/jats:italic>\n incubation residues. Most of the excreta InsP concentrations were lower in LB than in LSL (\n P<\/jats:italic>\n \u2264 0.010), higher at 30 than 42 weeks (\n P<\/jats:italic>\n &lt; 0.001), and higher when fed P+ than P\u2212 (\n P<\/jats:italic>\n &lt; 0.001). In the ileum, the InsP\n 6<\/jats:sub>\n concentration was lower in LB fed P+ and in LSL fed P\u2212 than in other treatments (\n P<\/jats:italic>\n = 0.027), while InsP\n 5<\/jats:sub>\n isomer concentrations varied with age \u00d7 strain interaction and age (\n P<\/jats:italic>\n \u2264 0.021). The MI concentration was higher in LB hens fed P\u2212 than LSL hens and hens fed P+ (\n P<\/jats:italic>\n \u2264 0.005). After\n in vitro<\/jats:italic>\n incubation, the InsP\n 6<\/jats:sub>\n concentration was higher with mucosa of 30-week-old hens fed P+ than in other treatments (\n P<\/jats:italic>\n = 0.016). The InsP\n 6<\/jats:sub>\n concentration after\n in vitro<\/jats:italic>\n incubation was highly heritable only in LB (\n \n \n \n \n h<\/mml:mi>\n 2<\/mml:mn>\n <\/mml:msup>\n <\/mml:mrow>\n <\/mml:math>\n <\/jats:inline-formula>\n = 0.62,\n P<\/jats:italic>\n = 0.003) and a polygenic structure for this trait was detected. The consistent results from excreta, ileum digesta, and\n in vitro<\/jats:italic>\n measures provide an extended view of endogenous mucosal phytate degradation primarily driven by a 6-phytase. Dietary P renunciation and aging of hens appeared to increase phytate degradation, possibly by increased expression of endogenous mucosal phosphatases. The differences between the two laying hen strains reveal different endogenous mechanisms and were reflected at the quantitative genetic level for the\n in vitro<\/jats:italic>\n traits.\n <\/jats:p>","DOI":"10.3389\/fphys.2026.1730157","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T05:29:17Z","timestamp":1773034157000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic, age, and diet effects on phytate degradation of laying hens studied in combined in vivo and in vitro assays"],"prefix":"10.3389","volume":"17","author":[{"given":"Anna","family":"Hanauska","sequence":"first","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim","place":["Stuttgart, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Sommerfeld","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim","place":["Stuttgart, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Schmid","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim","place":["Stuttgart, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentin P.","family":"Haas","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim","place":["Stuttgart, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Korinna","family":"Huber","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim","place":["Stuttgart, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rn","family":"Bennewitz","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim","place":["Stuttgart, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Rodehutscord","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, University of Hohenheim","place":["Stuttgart, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.4081\/ijas.2012.e8","article-title":"Phytate phosphorus utilization and intestinal phytase activity in laying hens","author":"Abudabos","year":"2012","journal-title":"It. J. Anim. Sci"},{"key":"B2","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1080\/17450390903052623","article-title":"Determination of titanium dioxide supplements in different matrices using two methods involving photometer and inductively coupled plasma optical emission spectrometer measurements","volume":"63","author":"Boguhn","year":"2009","journal-title":"Arch. Anim. Nutr."},{"key":"B3","volume-title":"Fits the linear mixed model: Asreml-r package version 4.1.0.160. Hemel hempstead","author":"Butler","year":"2017"},{"key":"B4","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1093\/ps\/77.6.850","article-title":"The effects of 1,25-dihydroxycholecalciferol and phytase on the natural phytate phosphorus utilization by laying hens","volume":"77","author":"Carlos","year":"1998","journal-title":"Poult. Sci."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1280","DOI":"10.3382\/ps.0491280","article-title":"Intestinal phytase and alkaline phosphatase of chicks: influence of dietary calcium, inorganic and phytate phosphorus and vitamin D3","volume":"49","author":"Davies","year":"1970","journal-title":"Poult. Sci."},{"key":"B6","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/0377-8401(94)90156-2","article-title":"Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs","volume":"47","author":"Eeckhout","year":"1994","journal-title":"Anim. Feed Sci. Technol."},{"key":"B7","volume-title":"Empfehlungen zur Energie-und N\u00e4hrstoffversorgung der Legehennen und Masth\u00fchner (Broiler)","author":"Ern\u00e4hrungsphysiologie","year":"1999"},{"key":"B8","doi-asserted-by":"publisher","first-page":"1564","DOI":"10.3382\/ps.0701564","article-title":"The effects of various dietary phosphorus levels on the circadian patterns of plasma 1,25-dihydroxycholecalciferol, total calcium, ionized calcium, and phosphorus in laying hens","volume":"70","author":"Frost","year":"1991","journal-title":"Poult. Sci."},{"key":"B9","doi-asserted-by":"publisher","first-page":"100867","DOI":"10.1016\/j.animal.2023.100867","article-title":"A dynamic model to measure retention of solid and liquid digesta fractions in chickens fed diets with differing fibre sources","volume":"17","author":"Gar\u00e7on","year":"2023","journal-title":"Animal"},{"key":"B10","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1006\/abbi.1993.1261","article-title":"Purification and characterization of two phytases from Escherichia coli","volume":"303","author":"Greiner","year":"1993","journal-title":"Arch. Biochem. Biophys."},{"key":"B11","doi-asserted-by":"publisher","first-page":"104333","DOI":"10.1016\/j.psj.2024.104333","article-title":"Gastrointestinal tract morphology, nutrient digestibility, and digesta mean retention time in laying hens of two different breeds fed with fine or coarse oat hulls","volume":"103","author":"Habibi","year":"2024","journal-title":"Poult. Sci."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1581088","DOI":"10.3389\/fphys.2025.1581088","article-title":"Endogenous mucosal phosphatases characterization in duodenum brush border membrane of laying hens","volume":"16","author":"Hanauska","year":"2025","journal-title":"Front. Physiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.3382\/ps\/pev065","article-title":"Modulation of small intestinal phosphate transporter by dietary supplements of mineral phosphorus and phytase in broilers","volume":"94","author":"Huber","year":"2015","journal-title":"Poult. Sci."},{"key":"B14","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1093\/ps\/77.4.557","article-title":"Phytase activity in the small intestinal brush border membrane of the chicken","volume":"77","author":"Maenz","year":"1998","journal-title":"Poult. Sci."},{"key":"B15","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1007\/BF00494365","article-title":"The influence of freezing and freeze-drying of tissue specimens on enzyme activity","volume":"51","author":"Meijer","year":"1977","journal-title":"Histochem"},{"key":"B16","doi-asserted-by":"publisher","first-page":"2577","DOI":"10.1021\/bm900523m","article-title":"A new surface for immobilizing and maintaining the function of enzymes in a freeze-dried state","volume":"10","author":"Nosworthy","year":"2009","journal-title":"Biomacromolecules"},{"key":"B17","volume-title":"R: a language and environment for statistical computing","year":"2021"},{"key":"B18","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1186\/s12864-021-07814-9","article-title":"Transcriptional responses in jejunum of two layer chicken strains following variations in dietary calcium and phosphorus levels","volume":"22","author":"Reyer","year":"2021","journal-title":"BMC Genomics"},{"key":"B19","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1079\/9781789241563.0008","article-title":"Phytases: potential and limits of phytate destruction in the digestive tract of pigs and poultry","volume-title":"Enzymes in farm animal nutrition","author":"Rodehutscord","year":"2022"},{"key":"B20","doi-asserted-by":"publisher","first-page":"951350","DOI":"10.3389\/fphys.2022.951350","article-title":"The active core microbiota of two high-yielding laying hen breeds fed with different levels of calcium and phosphorus","volume":"13","author":"Roth","year":"2022","journal-title":"Front. Physiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"3012","DOI":"10.3390\/ani11113012","article-title":"Changes in blood metabolites, intestinal microbiota composition and gene expression of 95 weeks old laying hens differing in egg production and egg breaking strength","volume":"11","author":"Schokker","year":"2021","journal-title":"Animals"},{"key":"B22","doi-asserted-by":"publisher","first-page":"e13484","DOI":"10.1111\/asj.13484","article-title":"Effect of low-temperature drying on the nitrogenous compounds and inositol phosphates in broiler chickens and cecectomized laying hen excreta","volume":"92","author":"Siegert","year":"2021","journal-title":"Anim. Sci. J."},{"key":"B23","doi-asserted-by":"publisher","first-page":"102470","DOI":"10.1016\/j.psj.2022.102470","article-title":"Research note: influence of monocalcium phosphate and phytase in the diet on phytate degradation in cecectomized laying hens","volume":"102","author":"Siegert","year":"2023","journal-title":"Poult. Sci."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.3382\/ps\/pex404","article-title":"Interactive effects of phosphorus, calcium, and phytase supplements on products of phytate degradation in the digestive tract of broiler chickens","volume":"97","author":"Sommerfeld","year":"2018","journal-title":"Poult. Sci."},{"key":"B25","doi-asserted-by":"publisher","first-page":"6797","DOI":"10.1016\/j.psj.2020.08.064","article-title":"Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods","volume":"99","author":"Sommerfeld","year":"","journal-title":"Poult. Sci."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1736","DOI":"10.3390\/ani10101736","article-title":"Phytate degradation, transcellular mineral transporters, and mineral utilization by two strains of laying hens as affected by dietary phosphorus and calcium","volume":"10","author":"Sommerfeld","year":"","journal-title":"Animals"},{"key":"B27","doi-asserted-by":"publisher","first-page":"104407","DOI":"10.1016\/j.psj.2024.104407","article-title":"Effects of feeding diets without mineral P supplement on intestinal phytate degradation, blood concentrations of Ca and P, and excretion of Ca and P in two laying hen strains before and after onset of laying activity","volume":"103","author":"Sommerfeld","year":"2024","journal-title":"Poult. Sci."},{"key":"B28","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1017\/S0043933913000287","article-title":"Function and nutritional roles of the Avian caeca: a review","volume":"69","author":"Svihus","year":"2013","journal-title":"World\u2019s Poult. Sci. J."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1535","DOI":"10.1093\/ps\/76.11.1535","article-title":"The efficacy of phytase in corn-soybean meal-based diets for laying hens","volume":"76","author":"Van der Klis","year":"1997","journal-title":"Poult. Sci."},{"key":"B30","doi-asserted-by":"publisher","first-page":"4414","DOI":"10.3168\/jds.2007-0980","article-title":"Efficient methods to compute genomic predictions","volume":"91","author":"VanRaden","year":"2008","journal-title":"J. Dairy Sci."},{"key":"B31","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1113\/expphysiol.1989.sp003357","article-title":"Factors determining gastrointestinal transit time of several markers in the domestic fowl","volume":"74","author":"Vergara","year":"1989","journal-title":"Q. J. Exp. Physiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"e1","DOI":"10.1017\/jns.2014.62","article-title":"Hydrolysis of phytate and formation of inositol phosphate isomers without or with supplemented phytases in different segments of the digestive tract of broilers","volume":"4","author":"Zeller","year":"2015","journal-title":"J. Nutr. Sci."},{"key":"B33","doi-asserted-by":"publisher","first-page":"5065","DOI":"10.1016\/j.psj.2020.06.049","article-title":"Research note: delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers","volume":"99","author":"Zhai","year":"2020","journal-title":"Poult. Sci."}],"container-title":["Frontiers in Physiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphys.2026.1730157\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T05:29:18Z","timestamp":1773034158000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphys.2026.1730157\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,9]]},"references-count":33,"alternative-id":["10.3389\/fphys.2026.1730157"],"URL":"https:\/\/doi.org\/10.3389\/fphys.2026.1730157","relation":{},"ISSN":["1664-042X"],"issn-type":[{"value":"1664-042X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,9]]},"article-number":"1730157"}}