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Physiol."],"abstract":"<jats:p>\n                    Faithfully recapitulating the complex pathology of human tumors is a critical requirement for effective cancer research. However, animal models are limited by interspecies differences, and traditional\n                    <jats:italic>in vitro<\/jats:italic>\n                    systems often fail to maintain the native tissue architecture. Precision-cut tissue slices that retain the original tissue\u2019s histology, cellular heterogeneity, and physiological functions offer a compelling alternative. Cultured precision-cut tumor slices (PCTS) leverage these advantages to provide a highly representative model for investigating tumor biology and advancing therapeutic development. This review details PCTS, from tissue preparation and optimized culture conditions to their diverse applications in mechanistic research and preclinical drug evaluations.\n                  <\/jats:p>","DOI":"10.3389\/fphys.2026.1848297","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:40:50Z","timestamp":1789987250000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Precision-cut tumor slices: a biomimetic tool for accelerating oncological development from basic research to clinical translation"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiaojun","family":"Lei","sequence":"first","affiliation":[{"name":"School and Hospital of Stomatology, Dalian Medical University","place":["Dalian, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Bao","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology, Dalian Medical University","place":["Dalian, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Jin","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology, Dalian Medical University","place":["Dalian, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinwen","family":"Zhou","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology, Dalian Medical University","place":["Dalian, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Guo","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Dalian Medical University","place":["Dalian, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Kong","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology, Dalian Medical University","place":["Dalian, Liaoning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"B6","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s12929-024-00994-y","article-title":"Scaffold-based 3D cell culture models in cancer research","volume":"31","author":"Abuwatfa","year":"2024","journal-title":"J. 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