{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:44:18Z","timestamp":1791524658575,"version":"4.3.4"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>\n                    Sarcopenia, characterized by progressive loss of skeletal muscle mass and function, is exacerbated by chronic glucocorticoid exposure, which activates catabolic signaling pathways and accelerates muscle protein degradation. Although\n                    <jats:italic>Cryptotaenia japonica<\/jats:italic>\n                    Hassk (Apiaceae) has been reported to possess antioxidant and anti-inflammatory properties, its role in glucocorticoid-induced muscle atrophy remains unclear. In this study, we investigated the myoprotective effects of\n                    <jats:italic>Cryptotaenia japonica<\/jats:italic>\n                    extract (CJE) using both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    models. C2C12 myotubes were treated with dexamethasone (Dex, 10 \u03bcM) in the presence or absence of CJE (10\u2013100 \u03bcg\/mL), and key signaling pathways were analyzed by Western blotting and confocal microscopy.\n                    <jats:italic>In vivo<\/jats:italic>\n                    , Dex-induced muscle atrophy was established in ICR mice, followed by oral administration of CJE (200 mg\/kg\/day). Muscle tissues were evaluated for protein expression, histological alterations, and serum GDF-8 levels. CJE treatment attenuated Dex-induced upregulation of the E3 ubiquitin ligases MuRF-1 and FBX32 and inhibited FOXO1 nuclear translocation in C2C12 myotubes. In dexamethasone-treated mice, CJE restored mTOR phosphorylation and normalized the dexamethasone-induced dysregulation of AKT phosphorylation, while suppressing the FOXO\/E3 ubiquitin ligase catabolic axis. In Dex-treated mice, CJE reduced the expression of FOXO3a, MuRF-1, and FBX32, preserved muscle fiber architecture, and increased muscle fiber cross-sectional area. Furthermore, CJE modulated circulating GDF-8 levels associated with muscle atrophy. Collectively, these findings demonstrate that CJE mitigates glucocorticoid-induced muscle atrophy by coordinately regulating the AKT\/mTOR\u2013FOXO\/E3 ubiquitin ligase signaling axis. These results suggest that CJE may serve as a promising natural therapeutic candidate for the prevention of sarcopenia and muscle-wasting conditions.\n                  <\/jats:p>","DOI":"10.3389\/fphys.2026.1860064","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T04:20:45Z","timestamp":1784175645000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Cryptotaenia japonica extract attenuates glucocorticoid-induced muscle atrophy via regulation of the AKT\/mTOR\u2013FOXO\/E3 ubiquitin ligase axis"],"prefix":"10.3389","volume":"17","author":[{"given":"Da-In","family":"Choi","sequence":"first","affiliation":[{"name":"Well-Aging Medicare & City G-LAMP Project Group, Chosun University","place":["Gwangju, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HuiJun","family":"Lee","sequence":"additional","affiliation":[{"name":"Well-Aging Medicare & City G-LAMP Project Group, Chosun University","place":["Gwangju, Republic of Korea"]},{"name":"Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University","place":["Gwangju, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seokhoon","family":"Heo","sequence":"additional","affiliation":[{"name":"Well-Aging Medicare & City G-LAMP Project Group, Chosun University","place":["Gwangju, Republic of Korea"]},{"name":"Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University","place":["Gwangju, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subin","family":"Cho","sequence":"additional","affiliation":[{"name":"Well-Aging Medicare & City G-LAMP Project Group, Chosun University","place":["Gwangju, Republic of Korea"]},{"name":"Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University","place":["Gwangju, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pansoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Orgasis Corp.","place":["Suwon, Gyeonggi, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chul-Yung","family":"Choi","sequence":"additional","affiliation":[{"name":"Well-Aging Medicare & City G-LAMP Project Group, Chosun University","place":["Gwangju, Republic of Korea"]},{"name":"Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University","place":["Gwangju, Republic of Korea"]},{"name":"Department of Biomedical Science, Chosun University","place":["Gwangju, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"39597","DOI":"10.1074\/jbc.m110.129718","article-title":"Muscle wasting in aged, sarcopenic rats is associated with enhanced activity of the ubiquitin proteasome pathway","volume":"285","author":"Altun","year":"2010","journal-title":"J. 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