{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:50:26Z","timestamp":1791521426818,"version":"4.3.4"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Physiol."],"abstract":"<jats:p>\n                    <jats:italic>Plutella xylostella<\/jats:italic>\n                    is a destructive pest damaging the cruciferous plants worldwide. Transgenic plants expressing dsRNA can be widely deployed for pest control. However, highly effective lethal target genes are currently lacking. Here, we assessed the efficacy of RNA interference (RNAi) against\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    through microinjection of\n                    <jats:italic>in vitro<\/jats:italic>\n                    synthesized dsRNA targeting\n                    <jats:italic>PxvATPaseA<\/jats:italic>\n                    . The full-length ORF of\n                    <jats:italic>PxvATPaseA<\/jats:italic>\n                    was 1848\u00a0bp, encoding 615 amino acids.\n                    <jats:italic>PxvATPaseA<\/jats:italic>\n                    was widely transcribed during different developmental stages from egg to adult. It was abundantly expressed in the foregut, hindgut, and Malpighian tubules, compared with other tissue types. Injecting 800 ng of ds\n                    <jats:italic>PxvATPaseA<\/jats:italic>\n                    into the fourth-instar larvae greatly reduced the corresponding mRNA level by 1.88-fold on day 2, causing 45.8% larval mortality, and lastly 54.2% treated larvae successfully pupated. At a higher dose of 1200 ng of ds\n                    <jats:italic>PxvATPaseA<\/jats:italic>\n                    , RNAi efficacy was significantly enhanced. The expression level of\n                    <jats:italic>PxvATPaseA was<\/jats:italic>\n                    reduced by 2.22-fold and 1.92-fold on day 2 and 3, respectively, leading to 79.2% larval lethality, and only 20.8% of depleted larvae pupated. Our results indicate that\n                    <jats:italic>PxvATPaseA<\/jats:italic>\n                    is an effective target for managing\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    . These findings will facilitate the development of new insecticides and the cultivation of pest-resistant plants.\n                  <\/jats:p>","DOI":"10.3389\/fphys.2026.1974591","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:26:04Z","timestamp":1791519964000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Knockdown of vATPase subunit A impairs larval-pupal development in Plutella xylostella"],"prefix":"10.3389","volume":"17","author":[{"given":"Xuetao","family":"Yu","sequence":"first","affiliation":[{"name":"Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs","place":["Wuhan, China"]},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Xining, Plant Protection of Observation and Research Station","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Yazhouwan National Laboratory","place":["Sanya, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijie","family":"Ren","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs","place":["Wuhan, China"]},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Xining, Plant Protection of Observation and Research Station","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Luo","sequence":"additional","affiliation":[{"name":"Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences","place":["Enshi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanhao","family":"Dai","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs","place":["Wuhan, China"]},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Xining, Plant Protection of Observation and Research Station","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Lu","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs","place":["Wuhan, China"]},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Xining, Plant Protection of Observation and Research Station","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ren","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs","place":["Wuhan, China"]},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Xining, Plant Protection of Observation and Research Station","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhu","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs","place":["Wuhan, China"]},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Xining, Plant Protection of Observation and Research Station","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Li","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs","place":["Wuhan, China"]},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Xining, Plant Protection of Observation and Research Station","place":["Xining, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qidi","family":"Wei","sequence":"additional","affiliation":[{"name":"Guizhou Institute of Crop Germplasm Resources","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Wu","sequence":"additional","affiliation":[{"name":"Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs","place":["Wuhan, China"]},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, 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