{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:26:48Z","timestamp":1790724408579,"version":"4.1.0"},"reference-count":73,"publisher":"Frontiers Media SA","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Plant Sci."],"DOI":"10.3389\/fpls.2015.00402","type":"journal-article","created":{"date-parts":[[2015,6,18]],"date-time":"2015-06-18T03:40:58Z","timestamp":1434598858000},"source":"Crossref","is-referenced-by-count":33,"title":["Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association"],"prefix":"10.3389","volume":"6","author":[{"given":"Ana M.","family":"Castillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa A.","family":"S\u00c3\u00a1nchez-D\u00c3\u00adaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00c3\u00ada P.","family":"Vall\u00c3\u00a9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2015,6,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/s11240-013-0379-y","article-title":"Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat","volume":"116","author":"Asif","year":"2014","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"B3","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1078\/0171-9335-00378","article-title":"Characterization of proteins secreted during maize microspore culture: arabinogalactan proteins (AGPs) stimulate embryo development","volume":"83","author":"Borderies","year":"2004","journal-title":"Eur. J. Cell Biol"},{"key":"B4","first-page":"73","article-title":"Biochemical and molecular aspects of haploid embryogenesis,","volume-title":"Biotechnology in Agriculture and Forestry, Haploids in Crop Improvement II","author":"Boutilier","year":"2005"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1737","DOI":"10.1105\/tpc.001941","article-title":"Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth","volume":"14","author":"Boutilier","year":"2002","journal-title":"Plant Cell"},{"key":"B6","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/s11240-008-9432-7","article-title":"Ovary co-culture improves embryo and green plant production in anther culture of Australian wheat (Triticum aestivum L.)","volume":"95","author":"Broughton","year":"2008","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"B7","first-page":"401","article-title":"Isolated microspore culture in wheat (Triticum aestivum L.): the effect of co-culture of wheat or barley ovaries on embryogenesis","volume":"24","author":"Bruins","year":"1996","journal-title":"Cereal Res. Commun"},{"key":"B8","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/978-1-4020-8854-4_27","article-title":"Chromosome doubling in monocots,","volume-title":"Advances in Haploid Production in Higher Plants","author":"Castillo","year":"2009"},{"key":"B9","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1111\/j.1467-7652.2010.00561.x","article-title":"Use of doubled haploid technology for development of stable drought tolerant bread wheat (Triticum aestivum L.) transgenics","volume":"9","author":"Chauhan","year":"2011","journal-title":"Plant Biotechnol. J"},{"key":"B10","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/0092-8674(95)90427-1","article-title":"A floral transmitting tissue-specific glycoprotein attracts pollen tubes and stimulates their growth","volume":"82","author":"Cheung","year":"1995","journal-title":"Cell"},{"key":"B11","doi-asserted-by":"publisher","first-page":"455","DOI":"10.4161\/psb.3.7.5601","article-title":"Arabinogalactan proteins (AGPs) related to pollen tube guidance into the embryo sac in Arabidopsis","volume":"3","author":"Coimbra","year":"2008","journal-title":"Plant Signal Behav"},{"key":"B12","first-page":"1870","article-title":"Androgenic haploids: factors controlling development and its application in crop improvement","volume":"89","author":"Datta","year":"2005","journal-title":"Curr. Sci"},{"key":"B13","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/0168-9452(87)90069-0","article-title":"Isolated microspore derived plant formation via embryogenesis in Triticum aestivum L","volume":"48","author":"Datta","year":"1987","journal-title":"Plant Sci"},{"key":"B14","doi-asserted-by":"publisher","first-page":"3129","DOI":"10.1038\/ncomms4129","article-title":"Reactive oxygen species mediate pollen tube rupture to release sperm for fertilization in Arabidopsis","volume":"5","author":"Duan","year":"2014","journal-title":"Nat. Commun"},{"key":"B15","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1007\/s00497-013-0217-8","article-title":"Arabinogalactan protein profiles and distribution patterns during microspore embryogenesis and pollen development in Brassica napus","volume":"26","author":"El-Tantawy","year":"2013","journal-title":"Plant Reprod"},{"key":"B16","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1126\/science.1143562","article-title":"The FERONIA receptor-like kinase mediates male-female interactions during pollen tube reception","volume":"317","author":"Escobar-Restrepo","year":"2007","journal-title":"Science"},{"key":"B17","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1007\/s00438-006-0159-z","article-title":"Putative fasciclin-like arabinogalactan-proteins (FLA) in wheat (Triticum aestivum) and rice (Oryza sativa): identification and bioinformatics analyses","volume":"276","author":"Faik","year":"2006","journal-title":"Mol Genet Genomics"},{"key":"B18","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1007\/s11240-010-9770-0","article-title":"Microspore embryogenesis in Apiaceae","volume":"104","author":"Ferrie","year":"2011","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"B19","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1079\/IVP2005690","article-title":"Effects of brassinosteroids on microspore embryogenesis in Brassica species","volume":"41","author":"Ferrie","year":"2005","journal-title":"In Vitro Cell. Dev. Biol. Plant"},{"key":"B20","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1016\/j.tplants.2007.06.007","article-title":"The resurgence of haploids in higher plants","volume":"12","author":"Forster","year":"2007","journal-title":"Trends Plant Sci"},{"key":"B21","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1007\/s11240-010-9852-z","article-title":"Anther culture for haploid and doubled haploid production","volume":"104","author":"German\u00c3\u00a0","year":"2011","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"B22","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/s00425-005-1515-7","article-title":"Three-dimensional confocal and electr\u00c3\u00b3n microscopy imaging define the dynamics and mechanism of diploidisation at early stages of barley microspore-derived embriog\u00c3\u00a9nesis","volume":"222","author":"Gonz\u00c3\u00a1lez-Melendi","year":"2005","journal-title":"Planta"},{"key":"B23","doi-asserted-by":"publisher","first-page":"7648","DOI":"10.1073\/pnas.0812346106","article-title":"Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana","volume":"106","author":"Guo","year":"2009","journal-title":"Proc. Natl Acad. Sci. U.S.A"},{"key":"B24","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1126\/science.1244454","article-title":"A peptide hormone and its receptor protein kinase regulate plant cell expansion","volume":"343","author":"Haruta","year":"2014","journal-title":"Science"},{"key":"B25","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/S0168-9452(97)00096-4","article-title":"Adrogenesis in Hordeum vulgare L.: effects of mannitol, calcium and abscisic acid on anther pretreatment","volume":"126","author":"Hoekstra","year":"1997","journal-title":"Plant Sci"},{"key":"B26","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1093\/pcp\/pcu182","article-title":"The Yin and Yang of cell wall integrity control: brassinosteroid and FERONIA","volume":"56","author":"Hofte","year":"2015","journal-title":"Signal. Plant Cell Physiol"},{"key":"B27","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1007\/s002990050271","article-title":"Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture","volume":"16","author":"Hu","year":"1997","journal-title":"Plant Cell Rep"},{"key":"B28","doi-asserted-by":"publisher","first-page":"2149","DOI":"10.1242\/dev.00458","article-title":"The Arabidopsis mutant feronia disrupts the female gametophytic control of pollen tube reception","volume":"130","author":"Huck","year":"2003","journal-title":"Development"},{"key":"B24a","first-page":"1","volume-title":"Plant Generation Method","author":"Hunter","year":"1987"},{"key":"B29","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1023\/A:1006399512217","article-title":"Genotypic variation in anther culture and effect of ovary co-culture in durum wheat","volume":"59","author":"J'Aitil","year":"1999","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"B30","article-title":"Plant defence mechanisms are triggered in the anther during the pre-treatment process,","volume-title":"The International Conference \u00e2\u20ac\u02dcHaploids in Higher Plants III\u00e2\u20ac\u2122","author":"Jacquard","year":"2006"},{"key":"B31","doi-asserted-by":"publisher","first-page":"737","DOI":"10.2135\/cropsci2008.08.0462","article-title":"Haploidy in cultivated wheats: induction and utility in basic and applied research","volume":"49","author":"Jauhar","year":"2009","journal-title":"Crop Sci"},{"key":"B32","first-page":"299","article-title":"Monoploid production by chromosome elimination,","volume-title":"Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture","author":"Jensen","year":"1977"},{"key":"B33","doi-asserted-by":"publisher","first-page":"1911","DOI":"10.1104\/pp.103.031237","article-title":"The Fasciclin-Like Arabinogalactan Proteins of Arabidopsis. A Multigene Family of Putative Cell Adhesion Molecules","volume":"133","author":"Johnson","year":"2003","journal-title":"Plant Physiol"},{"key":"B34","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1104\/pp.107.098723","article-title":"Combined transcriptome and proteome analysis identifies pathways and markers associated with the establishment of rapeseed microspore-derived embryo development","volume":"144","author":"Joosen","year":"2007","journal-title":"Plant Physiol"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1227","DOI":"10.1093\/jexbot\/52.359.1227","article-title":"Nuclear fusion leads to chromosome doubling during mannitol pretreatment of barley (Hordeum vulagre L.) microspores","volume":"52","author":"Kasha","year":"2001","journal-title":"J. Exp. Bot"},{"key":"B36","doi-asserted-by":"publisher","first-page":"107","DOI":"10.15252\/embr.201438801","article-title":"Functional analysis of related CrRLK1L receptor-like kinases in pollen tube reception","volume":"16","author":"Kessler","year":"2015","journal-title":"EMBO Rep"},{"key":"B37","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/S0176-1617(00)80305-3","article-title":"Assesment and improvement of wheat microspore derived embryo induction and regeneration","volume":"156","author":"Kunz","year":"2000","journal-title":"J. Plant Physiol"},{"key":"B38","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1111\/pbr.12032","article-title":"Efficient application of in vitro anther culture for different European winter wheat (Triticum aestivum L.) breeding programmes","volume":"132","author":"Lantos","year":"2013","journal-title":"Plant Breed"},{"key":"B39","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1007\/s00299-005-0013-5","article-title":"Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture","volume":"24","author":"Letarte","year":"2006","journal-title":"Plant Cell Rep"},{"key":"B40","doi-asserted-by":"publisher","first-page":"686","DOI":"10.2135\/cropsci2002.0686","article-title":"Highly Efficient Doubled-Haploid production in wheat (Triticum aestivum L.) via induced microspore embryogenesis","volume":"42","author":"Liu","year":"2002","journal-title":"Crop Sci"},{"key":"B41","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u00ce\u201d\u00ce\u201dCT method","volume":"2","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"B42","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1007\/s11240-008-9338-4","article-title":"Improvement of isolated microspore culture of barley (Hordeum vulgare L.): the effect of floret co-culture","volume":"93","author":"Lu","year":"2008","journal-title":"Plant Cell Tiss Organ Cult"},{"key":"B43","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1104\/pp.106.092932","article-title":"Transcript profiling and identification of molecular markers for early microspore embryogenesis in Brassica napus","volume":"144","author":"Malik","year":"2007","journal-title":"Plant Physiol"},{"key":"B44","first-page":"23","article-title":"Secreted molecules and their role in embryo formation in plants: a mini-review","volume":"47\/1","author":"Matthys-Rochon","year":"2005","journal-title":"Acta Biol. Cracov. Series Bot"},{"key":"B45","first-page":"149","article-title":"Plant regeneration from isolated microspores of Triticum aestivum","volume":"12","author":"Mezja","year":"1993","journal-title":"Plant Cell Rep"},{"key":"B46","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1023\/A:1022805623167","article-title":"Proteins produced by barley microspores and their derived androgenic structures promote in vitro zygotic maize embryo formation","volume":"73","author":"Paire","year":"2003","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"B47","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1186\/1471-2199-10-11","article-title":"Identification and validation of reference genes for quantitaive RT-PCR normalization in wheat","volume":"10","author":"Paolacci","year":"2009","journal-title":"BMC Mol. Biol"},{"key":"B48","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1139\/b06-064","article-title":"When microspores decide to become embryos\u00e2\u20ac\u201dcellular and molecular changes","volume":"84","author":"Pauls","year":"2006","journal-title":"Can. J. Bot"},{"key":"B49","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/S0176-1617(99)80182-5","article-title":"Impact of explant type, duration and initiation time on the co-culture effect in isolated microspore culture of wheat (Triticum aestivum L.)","volume":"154","author":"Puolimatka","year":"1999","journal-title":"J. Plant Physiol"},{"key":"B50","first-page":"393","article-title":"Effect of ovary co-cultivation and culture medium on embryogenesys of direct isolated microspores of wheat","volume":"24","author":"Puolimatka","year":"1996","journal-title":"Cereal Res. Commun"},{"key":"B51","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1104\/pp.105.4.1455","article-title":"wali6 and wali7, Genes lnduced by Aluminum in Wheat (Trificum aestivum 1.) Roots","volume":"105","author":"Richards","year":"1994","journal-title":"Plant Physiol"},{"key":"B52","doi-asserted-by":"publisher","first-page":"1844","DOI":"10.1016\/j.bpj.2011.08.016","article-title":"Chemically mediated mechanical expansion of the pollen tube cell wall","volume":"101","author":"Rojas","year":"2011","journal-title":"Biophys. J"},{"key":"B53","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1016\/S0960-9822(03)00093-9","article-title":"Female control of male gamete delivery during fertilization in Arabidopsis thaliana","volume":"13","author":"Rotman","year":"2003","journal-title":"Curr. Biol"},{"key":"B54","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1007\/s00497-013-0225-8","article-title":"Microspore embryogenesis in wheat: new marker genes for early, middle and late stages of embryo development","volume":"26","author":"S\u00c3\u00a1nchez-D\u00c3\u00adaz","year":"2013","journal-title":"Plant Reprod"},{"key":"B55","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1105\/tpc.007872","article-title":"The Arabidopsis SOS5 locus encodes a putative cell surface adhesion protein and is required for normal cell expansion","volume":"15","author":"Shi","year":"2003","journal-title":"Plant Cell"},{"key":"B56","doi-asserted-by":"publisher","first-page":"1238","DOI":"10.1111\/jipb.12108","article-title":"Diverse Roles of ERECTA family genes in plant development","volume":"55","author":"Shpak","year":"2013","journal-title":"J Integr. Plant Biol"},{"key":"B57","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1007\/s00299-007-0500-y","article-title":"Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture","volume":"27","author":"Soriano","year":"2008","journal-title":"Plant Cell Rep"},{"key":"B58","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1007\/s11240-007-9288-2","article-title":"Effects of colchicine on anther and microspore culture of bread wheat (Triticum aestivum L.)","volume":"91","author":"Soriano","year":"2007","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"B59","first-page":"36","volume-title":"Methods and Applications of Doubled Haploid Technology in Wheat Breeding ICARDA","author":"Tadesse","year":"2013"},{"key":"B60","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1111\/tpj.12206","article-title":"Developmental transcriptional profiling reveals key insights into Triticeae reproductive development","volume":"74","author":"Tran","year":"2013","journal-title":"Plant J"},{"key":"B61","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1007\/s00425-006-0388-8","article-title":"Identification and characterization of genes expressed in early embryogenesis from microspores of Brassica napus","volume":"225","author":"Tsuwamoto","year":"2007","journal-title":"Planta"},{"key":"B62","doi-asserted-by":"publisher","first-page":"299","DOI":"10.3109\/10520298709108014","article-title":"Rapid assessment of microspore and pollen development stage in wheat and maize using DAPI and membrane permeabilization","volume":"62","author":"Vergne","year":"1987","journal-title":"Stain Technol"},{"key":"B63","doi-asserted-by":"crossref","first-page":"871","DOI":"10.4161\/psb.3.10.6227","article-title":"Role of complex N-glycans in plant stress tolerance","volume":"3","author":"von Schaewen","year":"2008","journal-title":"Plant Signal. Behav"},{"key":"B64","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1023\/A:1006383724443","article-title":"Characterization of cDNAs expressed in the early stages of microspore embryogenesis in barley (Hordeum vulgare L.)","volume":"41","author":"Vrinten","year":"1999","journal-title":"Plant Mol. Biol"},{"key":"B65","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1046\/j.1365-313X.2002.01313.x","article-title":"Male gametophyte development in bread wheat (Triticum aestivum L.): molecular, cellular, and biochemical analyses of a sporophytic contribution to pollen wall ontogeny","volume":"30","author":"Wang","year":"2002","journal-title":"Plant J"},{"key":"B66","doi-asserted-by":"publisher","first-page":"14693","DOI":"10.1073\/pnas.1212547109","article-title":"FERONIA receptor kinase pathway suppresses abscisic acid signaling in Arabidopsis by activating ABI2 phosphatase","volume":"109","author":"Yu","year":"2012","journal-title":"Proc. Natl Acad. Sci. U.S.A"},{"key":"B67","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1093\/mp\/ssu092","article-title":"From receptor-like kinases to calcium spikes: what are the missing links?","volume":"7","author":"Yu","year":"2014","journal-title":"Mol. Plant"},{"key":"B68","first-page":"190","article-title":"Advances in research of fasciclin-like arabinogalactan proteins (FLAs) in plants","volume":"8","author":"Zang","year":"2015","journal-title":"Plant Omics J"},{"key":"B69","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1023\/A:1023076213639","article-title":"Microspore culture in wheat (Triticum aestivum)-doubled haploid production via induced embryogenesis","volume":"73","author":"Zheng","year":"2003","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"B70","doi-asserted-by":"publisher","first-page":"802","DOI":"10.1007\/s00299-001-0411-2","article-title":"The effect of ovary-conditioned medium on microspore embryogenesis in common wheat (Triticum aestivum L.)","volume":"20","author":"Zheng","year":"2002","journal-title":"Plant Cell Rep"},{"key":"B71","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1007\/BF00236263","article-title":"Improved plant regeneration from wheat anther culture and barley microspore culture using phenyl acetic acid (PAA)","volume":"11","author":"Ziauddin","year":"1992","journal-title":"Plant Cell Rep"},{"key":"B72","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1007\/s00299-009-0730-2","article-title":"Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (\u00c3\u2014Triticosecale Wittm.)","volume":"28","author":"Zur","year":"2009","journal-title":"Plant Cell Rep"},{"key":"B73","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1007\/s11240-014-0515-3","article-title":"Antioxidant activity and ROS tolerance in triticale (xTriticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis","volume":"119","author":"Zur","year":"2014","journal-title":"Plant Cell Tissue Organ Cult"}],"container-title":["Frontiers in Plant Science"],"original-title":[],"deposited":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T09:59:15Z","timestamp":1498211955000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/Article\/10.3389\/fpls.2015.00402\/abstract"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,18]]},"references-count":73,"URL":"https:\/\/doi.org\/10.3389\/fpls.2015.00402","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,18]]}}}