{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T19:32:36Z","timestamp":1789500756650,"version":"build-2803163510"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T00:00:00Z","timestamp":1539820800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"DOI":"10.3389\/fpls.2018.01523","type":"journal-article","created":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T04:31:10Z","timestamp":1539837070000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":55,"title":["Development of Wheat With Hypoimmunogenic Gluten Obstructed by the Gene Editing Policy in Europe"],"prefix":"10.3389","volume":"9","author":[{"given":"Aur\u00e9lie","family":"Jouanin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesley","family":"Boyd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard G. F.","family":"Visser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marinus J. M.","family":"Smulders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,10,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1023\/b:euph.0000014914.85465.4f","article-title":"Global impact of mutation-derived varieties.","volume":"135","author":"Ahloowalia","year":"2004","journal-title":"Euphytica"},{"key":"B2","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1016\/j.tplants.2015.04.011","article-title":"Feasibility of new breeding techniques for organic farming.","volume":"20","author":"Andersen","year":"2015","journal-title":"Trends Plant Sci."},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/73200","article-title":"In vivo antigen challenge in celiac disease identifies a single transglutaminase-modified peptide as the dominant A-gliadin T-cell epitope.","volume":"6","author":"Anderson","year":"2000","journal-title":"Nat. Med."},{"key":"B4","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1016\/j.geoforum.2009.09.006","article-title":"Wheat as food, wheat as industrial substance; comparative geographies of transformation and mobility.","volume":"41","author":"Atchison","year":"2010","journal-title":"Geoforum"},{"key":"B5","first-page":"23","article-title":"\u201cPlant mutation breeding: current progress and future assessment,\u201d in","author":"Bado","year":"2015","journal-title":"Plant Breeding Reviews 39"},{"key":"B6","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.tibtech.2014.11.008","article-title":"Enabling plant synthetic biology through genome engineering.","volume":"33","author":"Baltes","year":"2015","journal-title":"Trends Biotechnol."},{"key":"B7","first-page":"23","article-title":"Protein composition and techno-functional properties of transgenic wheat with reduced alpha-gliadin content obtained by RNA interference.","volume":"85","author":"Becker","year":"2012","journal-title":"J. Appl. Bot. Food Qual."},{"key":"B8","author":"Belz","year":"2016","journal-title":"Reduction of Salt in Yeasted Wheat Bread: Impact on Bread Quality and Solutions using Sourdough Fermented by Functional Lactic Acid Bacteria Strains."},{"key":"B9","author":"Burger","year":"2018","journal-title":"Bayer, BASF to Pursue Plant Gene Editing Elsewhere After EU Ruling."},{"key":"B10","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/s00217-007-0702-0","article-title":"Evaluation of the nutritional quality of the lipid fraction of gluten-free biscuits.","volume":"227","author":"Caponio","year":"2008","journal-title":"Eur. Food Res. Technol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1111\/1541-4337.12091","article-title":"Novel approaches in gluten-free bread making: interface between food science, nutrition, and health.","volume":"13","author":"Capriles","year":"2014","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"B12","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1017\/CBO9780511998560.016","author":"Cheyne","year":"2012","journal-title":"Consumer Labelling in EU and WTO law. Liberalising Trade in EU and The WTO: A Legal Comparison."},{"key":"B13","first-page":"1","article-title":"Directive \/18\/EC of the European Parliament and of the Council of 12 March 2001 on the deliberate release into the environment of genetically modified organisms and repealing Council Directive 90\/220\/EEC\u2014Commission Declaration.","volume":"106","year":"2001","journal-title":"Official J."},{"key":"B14","year":"2015","journal-title":"EASAC Statement on New Breeding Techniques."},{"key":"B15","year":"2000","journal-title":"Communication from the Commission on the pre-Cautionary Principle: COM"},{"key":"B16","year":"","journal-title":"Judgement of the Court in Case C-528\/16."},{"key":"B17","year":"","journal-title":"Opinion of Advocate General Bobek in Case C-528\/16."},{"key":"B18","article-title":"Scientific opinion addressing the safety assessment of plants developed using zinc finger nuclease 3 and other site-directed nucleases with similar function.","volume":"10","year":"2012","journal-title":"EFSA J."},{"key":"B19","year":"2015","journal-title":"Genetically Modified Organisms UNIT. Mandate Number: M-2015-0183."},{"key":"B20","year":"2015","journal-title":"Crop Genetic Improvement Technologies."},{"key":"B21","year":"2016","journal-title":"Towards an Innovation Principle Endorsed by Better Regulation (EPSC Strategic Notes No. Issue 14)."},{"key":"B22","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1097\/01.mog.0000245543.72537.9e","article-title":"Systemic autoimmune disorders in celiac disease.","volume":"22","author":"Fasano","year":"2006","journal-title":"Curr. Opin. Gastroenterol."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090898","article-title":"Reduced-gliadin wheat bread: an alternative to the gluten-free diet for consumers suffering gluten-related pathologies.","volume":"9","author":"Gil-Humanes","year":"2014","journal-title":"PLoS One"},{"key":"B24","doi-asserted-by":"publisher","first-page":"17023","DOI":"10.1073\/pnas.1007773107","article-title":"Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference.","volume":"107","author":"Gil-Humanes","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B25","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1159\/000128995","article-title":"\u201cBeyond coeliac disease toxicity: detoxified and non-toxic grains. Pediatric and adolescent medicine,\u201d in","author":"Gilissen","year":"2008","journal-title":"Frontiers in Celiac Disease"},{"key":"B26","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.jcs.2014.01.005","article-title":"Reducing the incidence of allergy and intolerance to cereals.","volume":"59","author":"Gilissen","year":"2014","journal-title":"J. Cereal Sci."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1038\/498409a","article-title":"Europe should rethink its stance on GM crops.","volume":"498","author":"Heap","year":"2013","journal-title":"Nature"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3390\/foods5020030","article-title":"Fundamental study on the impact of gluten-free starches on the quality of gluten-free model breads.","volume":"5","author":"Horstmann","year":"2016","journal-title":"Foods"},{"key":"B29","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1097\/MPG.0b013e31821a23d0","article-title":"European society for pediatric gastroenterology, hepatology, and nutrition guidelines for the diagnosis of coeliac disease.","volume":"54","author":"Husby","year":"2012","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.1007\/s00299-016-1974-2","article-title":"Consumer acceptance of food crops developed by genome editing.","volume":"35","author":"Ishii","year":"2016","journal-title":"Plant Cell Rep."},{"key":"B31","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.foodres.2017.04.025","article-title":"Food processing and breeding strategies for coeliac-safe and healthy wheat products.","volume":"110","author":"Jouanin","year":"2018","journal-title":"Food Res. Int."},{"key":"B32","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1007\/s00281-012-0321-0","article-title":"Celiac disease: quantity matters.","volume":"34","author":"Koning","year":"2012","journal-title":"Semin. Immunopathol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1038\/nbt.3533","article-title":"Will Europe toast GM wheat for gluten sufferers?","volume":"34","author":"Laursen","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/S1470-2045(14)71174-X","article-title":"Silencing the voice of scientific reason.","volume":"16","author":"Lawler","year":"2015","journal-title":"Lancet Oncol."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14261","article-title":"Efficient DNA-free genome editing of bread wheat using CRISPR\/Cas9 ribonucleoprotein complexes.","volume":"8","author":"Liang","year":"2017","journal-title":"Nat. Commun."},{"key":"B36","doi-asserted-by":"publisher","first-page":"4254","DOI":"10.3390\/v7082819","article-title":"Public acceptance of plant biotechnology and GM crops.","volume":"7","author":"Lucht","year":"2015","journal-title":"Viruses"},{"key":"B37","author":"McDougall","year":"2011","journal-title":"The Cost and Time Involved in the Discovery, Development and Authorisation of a New Plant Biotechnology Derived Trait: A Consultancy Study for Crop Life International."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015637","article-title":"A universal approach to eliminate antigenic properties of alpha-gliadin peptides in celiac disease.","volume":"5","author":"Mitea","year":"2010","journal-title":"PLoS One"},{"key":"B39","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1093\/scipol\/scs093","article-title":"Responsible research and innovation: from science in society to science for society, with society.","volume":"39","author":"Owen","year":"2012","journal-title":"Sci. Public Policy"},{"key":"B40","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1111\/tpj.12851","article-title":"Diversification of the celiac disease \u03b1-gliadin complex in wheat: a 33-mer peptide with six overlapping epitopes, evolved following polyploidization.","volume":"82","author":"Ozuna","year":"2015","journal-title":"Plant J."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1643","DOI":"10.1016\/j.str.2016.07.010","article-title":"Diverse T cell receptor gene usage in HLA-DQ8-associated Celiac Disease converges into a consensus binding solution.","volume":"24","author":"Petersen","year":"2016","journal-title":"Structure"},{"key":"B42","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1038\/nsmb.2817","article-title":"T-cell receptor recognition of HLA- DQ2-gliadin complexes associated with celiac disease.","volume":"21","author":"Petersen","year":"2014","journal-title":"Nat. Struct. Mol. Biol."},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-905","article-title":"Quantitative and qualitative differences in celiac disease epitopes among durum wheat varieties identified through deep RNA-amplicon sequencing.","volume":"14","author":"Salentijn","year":"2013","journal-title":"BMC Genomics"},{"key":"B44","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1111\/pbi.12837","article-title":"Low-gluten, non-transgenic wheat engineered with CRISPR\/Cas9.","volume":"16","author":"S\u00e1nchez-Le\u00f3n","year":"2018","journal-title":"Plant Biotechnol. J."},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-10-13","article-title":"Spectrum of gluten-related disorders: consensus on new nomenclature and classification.","volume":"10","author":"Sapone","year":"2012","journal-title":"BMC Med."},{"key":"B46","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.plantsci.2015.09.011","article-title":"CRISPR\/Cas9-mediated genome editing and gene replacement in plants: transitioning from lab to field.","volume":"240","author":"Schaeffer","year":"2015","journal-title":"Plant Sci."},{"key":"B47","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.foodqual.2010.08.003","article-title":"Consumer attitudes towards hypoallergenic apples that alleviate mild apple allergy.","volume":"22","author":"Schenk","year":"2011","journal-title":"Food Qual. Prefer."},{"key":"B48","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1094\/9781891127557.008","article-title":"\u201cWheat grain proteins,\u201d in","author":"Shewry","year":"2009","journal-title":"Wheat: Chemistry and Technology"},{"key":"B49","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.jcs.2015.06.005","article-title":"Improving wheat to remove coeliac epitopes but retain functionality.","volume":"67","author":"Shewry","year":"2016","journal-title":"J. Cereal Sci."},{"key":"B50","author":"Simon","year":"2013","journal-title":"Chief EU Scientist Backs Damning Report Urging GMO \u2018rethink\u2019."},{"key":"B51","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.foodres.2017.11.031","article-title":"Oats in healthy gluten-free and regular diets: a perspective.","volume":"110","author":"Smulders","year":"2018","journal-title":"Food Res. Int."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1007\/s00299-016-1990-2","article-title":"Regulatory hurdles for genome editing: process-vs. product-based approaches in different regulatory contexts.","volume":"35","author":"Sprink","year":"","journal-title":"Plant Cell Rep."},{"key":"B53","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1007\/s11816-016-0418-3","article-title":"Plant genome editing in the European Union - to be or not to be - a GMO.","volume":"10","author":"Sprink","year":"","journal-title":"Plant Biotechnol. Rep."},{"key":"B54","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.copbio.2014.11.010","article-title":"Plant genome editing by novel tools: TALEN and other sequence specific nucleases.","volume":"32","author":"Sprink","year":"2015","journal-title":"Curr. Opin. Biotechnol."},{"key":"B55","year":"2012","journal-title":"Benefits and Risks of the Deliberate Release of Genetically Modified Plants. National Research Programme NRP 59."},{"key":"B56","doi-asserted-by":"publisher","first-page":"1139","DOI":"10.1111\/pbi.12482","article-title":"Creation of the first ultra-low gluten barley (Hordeum vulgare L.) for coeliac and gluten-intolerant populations.","volume":"14","author":"Tanner","year":"2016","journal-title":"Plant Biotechnol. J."},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001012","article-title":"Comprehensive, quantitative mapping of T cell epitopes in gluten in celiac disease.","volume":"2","author":"Tye-Din","year":"2010","journal-title":"Sci. Transl. Med."},{"key":"B58","doi-asserted-by":"publisher","first-page":"1527","DOI":"10.1007\/s00122-010-1408-4","article-title":"Presence of celiac disease epitopes in modern and old hexaploid wheat varieties: wheat breeding may have contributed to increased prevalence of celiac disease.","volume":"121","author":"Van den Broeck","year":"","journal-title":"Theor. Appl. Genet."},{"key":"B59","doi-asserted-by":"publisher","first-page":"2206","DOI":"10.1039\/c0mb00046a","article-title":"In search of tetraploid wheat accessions reduced in celiac disease-related gluten epitopes.","volume":"6","author":"Van den Broeck","year":"","journal-title":"Mol. Biosyst."},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-9-41","article-title":"Removing celiac disease-related gluten proteins from bread wheat while retaining technological properties: a study with Chinese Spring deletion lines.","volume":"9","author":"Van den Broeck","year":"2009","journal-title":"BMC Plant Biol."},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-1","article-title":"Alpha-gliadin genes from the A, B, and D genomes of wheat contain different sets of celiac disease epitopes.","volume":"7","author":"Van Herpen","year":"2006","journal-title":"BMC Genomics"},{"key":"B62","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1080\/21645698.2015.1114698","article-title":"Regulatory framework for gene editing and other new breeding techniques (NBTs) in Argentina.","volume":"6","author":"Whelan","year":"2015","journal-title":"GM Crops Food"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12617","article-title":"Efficient and transgene-free genome editing in wheat through transient expression of CRISPR\/Cas9 DNA or RNA.","volume":"7","author":"Zhang","year":"2016","journal-title":"Nat. Commun."}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fpls.2018.01523\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T04:31:15Z","timestamp":1539837075000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fpls.2018.01523\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,18]]},"references-count":63,"alternative-id":["10.3389\/fpls.2018.01523"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2018.01523","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,18]]},"article-number":"1523"}}