{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:32:57Z","timestamp":1788294777879,"version":"build-2803163510"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T00:00:00Z","timestamp":1611100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900260"],"award-info":[{"award-number":["31900260"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31930009"],"award-info":[{"award-number":["31930009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003395","name":"Shanghai Municipal Education Commission","doi-asserted-by":"publisher","award":["2019-01-07-00-02-E00006"],"award-info":[{"award-number":["2019-01-07-00-02-E00006"]}],"id":[{"id":"10.13039\/501100003395","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["18DZ2260500"],"award-info":[{"award-number":["18DZ2260500"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["17DZ2252700"],"award-info":[{"award-number":["17DZ2252700"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Magnesium (Mg) is an abundant and important cation in cells. Plants rely on Mg transporters to take up Mg from the soil, and then Mg is transported to anthers and other organs. Here, we showed that <jats:italic>MGT6+\/\u2212<\/jats:italic> plants display reduced fertility, while <jats:italic>mgt6<\/jats:italic> plants are fertile. <jats:italic>MGT6<\/jats:italic> is expressed in the anther at the early stages. Pollen mitosis and intine formation are impaired in aborted pollen grains (PGs) of <jats:italic>MGT6+\/\u2212<\/jats:italic> plants, which is similar to the defective pollen observed in <jats:italic>mgt5<\/jats:italic> and <jats:italic>mgt9<\/jats:italic> mutants. These results suggest that Mg deficiency leads to pollen abortion in <jats:italic>MGT6+\/\u2212<\/jats:italic> plants. Our data showed that <jats:italic>mgt6<\/jats:italic> organs including buds develop significantly slower and <jats:italic>mgt6<\/jats:italic> stamens accumulate a higher level of Mg, compared with wild-type (WT) and <jats:italic>MGT6+\/\u2212<\/jats:italic> plants. These results indicate that slower bud development allows <jats:italic>mgt6<\/jats:italic> to accumulate sufficient amounts of Mg in the pollen, explaining why <jats:italic>mgt6<\/jats:italic> is fertile. Furthermore, we found that <jats:italic>mgt6<\/jats:italic> can restore fertility of <jats:italic>mgt5<\/jats:italic>, which has been reported to be male sterile due to defects in Mg transport from the tapetum to microspores and that an additional Mg supply can restore its fertility. Interestingly, <jats:italic>mgt5<\/jats:italic> fertility is recovered when grown under short photoperiod conditions, which is a well-known factor regulating plant fertility. Taken together, these results demonstrate that slow development is a general mechanism to restore <jats:italic>mgt<\/jats:italic>s fertility, which allows other redundant magnesium transporter (MGT) members to transport sufficient Mg for pollen formation.<\/jats:p>","DOI":"10.3389\/fpls.2020.621338","type":"journal-article","created":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T05:51:56Z","timestamp":1611121916000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Slowing Development Facilitates Arabidopsis mgt Mutants to Accumulate Enough Magnesium for Pollen Formation and Fertility Restoration"],"prefix":"10.3389","volume":"11","author":[{"given":"Xiao-Feng","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue-Xue","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Qi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Hui","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Yi","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nai-Ying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Rong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong-Nan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,1,20]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"7028","DOI":"10.1021\/bi049617+","article-title":"Mutation and evolution of the magnesium-binding site of a class II aminoacyl-tRNA synthetase","volume":"43","author":"Ador","year":"2004","journal-title":"Biochemistry"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"117","DOI":"10.3109\/10520296909063335","article-title":"Differential staining of aborted and nonaborted pollen","volume":"44","author":"Alexander","year":"1969","journal-title":"Stain. Technol."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1021\/acs.biochem.6b01243","article-title":"Modulation of the Na, K-ATPase by magnesium ions","volume":"56","author":"Apell","year":"2017","journal-title":"Biochemistry"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1146\/annurev-arplant-042809-112312","article-title":"Genetic regulation of sporopollenin synthesis and pollen exine development","volume":"62","author":"Ariizumi","year":"2011","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1104\/pp.111.184853","article-title":"No primexine and plasma membrane undulation is essential for primexine deposition and plasma membrane undulation during microsporogenesis in Arabidopsis","volume":"158","author":"Chang","year":"2012","journal-title":"Plant Physiol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1038\/cr.2009.58","article-title":"Magnesium transporter AtMGT9 is essential for pollen development in Arabidopsis","volume":"19","author":"Chen","year":"2009","journal-title":"Cell Res."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1146\/annurev-arplant-050213-040119","article-title":"Male sterility and fertility restoration in crops","volume":"65","author":"Chen","year":"2014","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"17200","DOI":"10.1021\/ja4087769","article-title":"Importance of metal hydration on the selectivity of Mg2+ versus Ca2+ in magnesium ion channels","volume":"135","author":"Dudev","year":"2013","journal-title":"J. Am. Chem. 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Plant Reprod."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/s0092-8674(03)00556-7","article-title":"Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7","volume":"114","author":"Schmitz","year":"2003","journal-title":"Cell"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"2032","DOI":"10.1016\/j.bbamem.2013.05.019","article-title":"Membrane protein interactions between different Arabidopsis thaliana MRS2-type magnesium transporters are highly permissive","volume":"1828","author":"Schmitz","year":"2013","journal-title":"Biochim. Biophys. 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Bacteriol."},{"key":"ref30","doi-asserted-by":"publisher","first-page":"1584","DOI":"10.1016\/j.molp.2017.09.017","article-title":"The magnesium transporter MGT10 is essential for chloroplast development and photosynthesis in Arabidopsis thaliana","volume":"10","author":"Sun","year":"2017","journal-title":"Mol. 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