{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:13:59Z","timestamp":1788912839069,"version":"build-2803163510"},"reference-count":67,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T00:00:00Z","timestamp":1615766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000921","name":"European Cooperation in Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000921","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Grapevine trunk diseases (GTDs) are a serious and growing threat to vineyards worldwide. The need for innovative control tools persists since pesticides used against some GTDs have been banned and only methods to prevent infections or to reduce foliar symptoms have been developed so far. In this context, the application of imaging methods, already applied to study plant\u2013microbe interactions, represents an interesting approach to understand the effect of experimental treatments applied to reduce fungal colonization, on GTD-related pathogens activity. To this aim, trials were carried out to evaluate the efficacy of copper-based treatments, formulated with hydroxyapatite (HA) as co-adjuvant with innovative delivery properties, loaded with two different copper(II) compounds (tribasic sulfate and sulfate pentahydrate), and applied to grapevine propagation material to inhibit fungal wood colonization. The treated rootstock (<jats:italic>Vitis berlandieri<\/jats:italic> \u00d7 <jats:italic>Vitis riparia<\/jats:italic> cv. K5BB) and scion cuttings (<jats:italic>Vitis vinifera<\/jats:italic> L., cv. Chardonnay) had been inoculated with a strain of <jats:italic>Phaeoacremonium minimum<\/jats:italic> (<jats:italic>Pmi<\/jats:italic>) compared to uninoculated rootstocks. Experimental treatments were applied during the water-soaking process, comparing the copper(II) compounds pure or formulated with HA, to hydrate the cuttings. After callusing, grafted vines were grown under greenhouse conditions in a nursery and inoculated with <jats:italic>Pmi<\/jats:italic>::<jats:italic>gfp7<\/jats:italic> or with <jats:italic>Pmi<\/jats:italic> wild-type. Fifteen weeks post-inoculation, woody tissues close to the inoculation site were sampled to evaluate the efficiency of the treatments by studying the plant\u2013microbe interaction by confocal laser scanning microscopy (CLSM). Copper and further elements were also quantified in the same tissues immediately after the treatments and on the CLSM samples. Finally, the grapevine defense responses were studied in the leaves of cuttings treated with the same formulations. The present investigation confirmed the relevant interaction of <jats:italic>Pmi<\/jats:italic> and the related transformed strain on the vascular tissues of grafted vines. Furthermore, <jats:italic>in vitro<\/jats:italic> assay revealed (i) the fungistatic effect of HA and the reduced effect of Cu fungicide when combined with HA. <jats:italic>In planta<\/jats:italic> assays showed (ii) the reduction of <jats:italic>Pmi<\/jats:italic> infection in propagation material treated with HA-Cu formulations, (iii) the movement of HA-Cu formulations inside the plant tissues and their persistence over time, and (iv) the plant defense reaction following the treatment with pure HA or Cu, or combined.<\/jats:p>","DOI":"10.3389\/fpls.2021.649694","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T06:08:55Z","timestamp":1615788535000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["In planta Activity of Novel Copper(II)-Based Formulations to Inhibit the Esca-Associated Fungus Phaeoacremonium minimum in Grapevine Propagation Material"],"prefix":"10.3389","volume":"12","author":[{"given":"Enrico","family":"Battiston","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Compant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Livio","family":"Antonielli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Mondello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Cl\u00e9ment","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Simoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Di Marco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Mugnai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Fontaine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,3,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"75","DOI":"10.14601\/Phytopathol_Mediterr-1728","article-title":"Do fungal naphthalenones have a role in the development of Esca symptoms?","volume":"43","author":"Abou-Mansour","year":"2004","journal-title":"Phytopathol. Mediterr."},{"key":"B2","doi-asserted-by":"publisher","first-page":"92","DOI":"10.14601\/Phytopathol_Mediterr-1550","article-title":"Pathogenicity of three species of Phaeoacremonium spp. on grapevine in California.","volume":"39","author":"Adalat","year":"2000","journal-title":"Phytopathol. Mediterr."},{"key":"B3","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.cropro.2010.12.020","article-title":"Evaluation of fungicides to control Cylindrocarpon liriodendri and Cylindrocarpon macrodidymum in vitro, and their effect during the rooting phase in the grapevine propagation process.","volume":"30","author":"Alaniz","year":"2011","journal-title":"Crop Prot."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01564-17","article-title":"Use of endophytic and rhizosphere actinobacteria from grapevine plants to reduce nursery fungal graft infections that lead to young grapevine decline.","volume":"83","author":"\u00c1lvarez-P\u00e9rez","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1569","DOI":"10.3390\/toxins3121569","article-title":"Phytotoxins produced by fungi associated with grapevine trunk diseases.","volume":"3","author":"Andolfi","year":"2011","journal-title":"Toxins"},{"key":"B6","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1146\/annurev.arplant.55.031903.141701","article-title":"Reactive oxygen species: metabolism, oxidative stress, and signal transduction.","volume":"55","author":"Apel","year":"2004","journal-title":"Annu. Rev. Plant. Biol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.cropro.2016.07.014","article-title":"Antifungal activities of extracts produced by liquid fermentations of Chilean Stereum species against Botrytis cinerea (grey mould agent).","volume":"89","author":"Aqueveque","year":"2016","journal-title":"Crop Prot."},{"key":"B8","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1094\/PHYTO-96-1188","article-title":"Chitosan oligomers and copper sulfate induce grapevine defense reactions and resistance to gray mold and downy mildew.","volume":"96","author":"Aziz","year":"2006","journal-title":"Phytopathology"},{"key":"B9","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1094\/PHYTO-02-18-0033-R","article-title":"Innovative delivery of Cu(II) ions by a nanostructured hydroxyapatite: potential application in planta to enhance the sustainable control of Plasmopara viticola.","volume":"109","author":"Battiston","year":"2019","journal-title":"Phytopathology"},{"key":"B10","doi-asserted-by":"publisher","first-page":"1903","DOI":"10.1002\/ps.4892","article-title":"Functionalisation of a nano-structured hydroxyapatite with copper(II) compounds as pesticide: in situ TEM and ESEM observations of treated Vitis vinifera L. leaves.","volume":"74","author":"Battiston","year":"2018","journal-title":"Pest Man. Sci."},{"key":"B11","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1002\/ps.5605","article-title":"Evaluation of long-term protection from nursery to vineyard provided by Trichoderma atroviride SC1 against fungal grapevine trunk pathogens.","volume":"76","author":"Berbegal","year":"2020","journal-title":"Pest Manag. Sci."},{"key":"B12","doi-asserted-by":"publisher","first-page":"162","DOI":"10.14601\/Phytopathol_Mediterr-1792","article-title":"Transformation of fungal grapevine trunk disease pathogens with the Green Fluorescent Protein gene.","volume":"44","author":"Bradshaw","year":"2005","journal-title":"Phytopathol Mediterr."},{"key":"B13","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.pmpp.2007.04.008","article-title":"Effects of three Esca-associated fungi on Vitis vinifera L.: I. Characterization of secondary metabolites in culture media and host responses to the pathogens in calli.","volume":"69","author":"Bruno","year":"2006","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/j.pmpp.2008.02.005","article-title":"Effects of three Esca-associated fungi on Vitis vinifera L.: V. changes in the chemical and biological profile of xylem sap from diseased cv. Sangiovese vines.","volume":"71","author":"Bruno","year":"2007","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"459","DOI":"10.14601\/Phytopathol_Mediterr-23636","article-title":"Further evidence that calcium, magnesium and seaweed mixtures reduce grapevine leaf stripe symptoms and increase grape yields.","volume":"57","author":"Calzarano","year":"2018","journal-title":"Phytopathol. Mediterr."},{"key":"B16","doi-asserted-by":"publisher","first-page":"116","DOI":"10.14601\/Phytopathol_Mediterr-1725","article-title":"Benefit of fungicide treatment after trunk renewal of vines with different types of Esca necrosis.","volume":"43","author":"Calzarano","year":"2004","journal-title":"Phytopathol. Mediterr."},{"key":"B17","doi-asserted-by":"publisher","first-page":"543","DOI":"10.14601\/Phytopathol_Mediterr-15253","article-title":"Grapevine leaf stripe disease symptoms (Esca complex) are reduced by a nutrients and seaweed mixture.","volume":"53","author":"Calzarano","year":"2014","journal-title":"Phytopathol. Mediterr."},{"key":"B18","doi-asserted-by":"publisher","first-page":"29","DOI":"10.14601\/Phyto-11150","article-title":"Current knowledge on Grapevine Trunk Diseases with complex etiology: a systemic approach.","volume":"59","author":"Claverie","year":"2020","journal-title":"Phytopathol. Mediterr."},{"key":"B19","doi-asserted-by":"publisher","first-page":"50","DOI":"10.14601\/Phytopathol_Mediterr-1853","article-title":"Evaluation of a trunk injection technique to control grapevine wood diseases.","volume":"46","author":"Darrieutort","year":"2007","journal-title":"Phytopathol. Mediterr."},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01405","article-title":"Fungicides and the grapevine wood mycobiome: a case study on tracheomycotic ascomycete Phaeomoniella chlamydospora reveals potential for two novel control strategies.","volume":"10","author":"Del Frari","year":"2019","journal-title":"Front. Plant Sci."},{"key":"B21","doi-asserted-by":"publisher","first-page":"73","DOI":"10.14601\/Phytopathol_Mediterr-1851","article-title":"Applications of Trichoderma to prevent Phaeomoniella chlamydospora infections in organic nurseries.","volume":"46","author":"Di Marco","year":"2007","journal-title":"Phytopathol. Mediterr."},{"key":"B22","doi-asserted-by":"publisher","first-page":"108","DOI":"10.14601\/Phytopathol_Mediterr-1730","article-title":"Experiments on the control of Esca by Trichoderma.","volume":"43","author":"Di Marco","year":"2004","journal-title":"Phytopathol. Mediterr."},{"key":"B23","doi-asserted-by":"publisher","first-page":"300","DOI":"10.14601\/Phytopathol_Mediterr-10233","article-title":"First studies on the potential of a copper formulation for the control of leaf stripe disease within Esca complex in grapevine.","volume":"50","author":"Di Marco","year":"2011","journal-title":"Phytopathol. Mediterr."},{"key":"B24","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.cropro.2013.01.001","article-title":"Efficacy of paste and liquid fungicide formulations to protect pruning wounds against pathogens associated with grapevine trunk diseases in Chile.","volume":"46","author":"D\u00edaz","year":"2013","journal-title":"Crop Prot."},{"key":"B25","doi-asserted-by":"publisher","first-page":"91","DOI":"10.14601\/Phytopathol_Mediterr-1860","article-title":"Preliminary trials on treatment of Esca-infected grapevines with trunk injection of fungicides.","volume":"46","author":"Dula","year":"2007","journal-title":"Phytopathol. Mediterr."},{"key":"B26","doi-asserted-by":"publisher","first-page":"387","DOI":"10.14601\/Phytopathol_Mediterr-1623","article-title":"Histological investigations on infection of grape roots and shoots by Phaeoacremonium spp.","volume":"40","author":"Feliciano","year":"2001","journal-title":"Phytopathol. Mediterr."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201802315","article-title":"Targeted drug delivery in plants: enzyme-responsive lignin nanocarriers for the curative treatment of the worldwide grapevine trunk disease Esca.","volume":"6","author":"Fischer","year":"2019","journal-title":"Adv. Sci."},{"key":"B28","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1111\/j.1755-0238.2010.00106.x","article-title":"Diagnosis of grapevine Esca disease by immunonological detection of Phaeomoniella chlamydospora.","volume":"16","author":"Fleurat-Lessard","year":"2010","journal-title":"Aust. J. Grape Wine Res."},{"key":"B29","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1007\/s10658-013-0372-7","article-title":"Immunological detection of Phaeoacremonium aleophilum, a fungal pathogen found in Esca disease.","volume":"139","author":"Fleurat-Lessard","year":"2014","journal-title":"Eur. J. Plant Pathol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1007\/s10658-015-0770-0","article-title":"The effects of grapevine trunk diseases (GTDs) on vine physiology.","volume":"144","author":"Fontaine","year":"2016","journal-title":"Eur. J. Plant. Pathol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1094\/PDIS-01-11-0025","article-title":"Fungal trunk pathogens in the grapevine propagation process: potential inoculum sources, detection, identification, and management strategies.","volume":"95","author":"Gramaje","year":"2011","journal-title":"Plant Dis."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1091","DOI":"10.1016\/j.cropro.2009.05.010","article-title":"Evaluation of fungicides to control Petri disease pathogens in the grapevine propagation process.","volume":"28","author":"Gramaje","year":"2009","journal-title":"Crop Prot."},{"key":"B33","doi-asserted-by":"publisher","first-page":"313","DOI":"10.14601\/Phytopathol_Mediterr-16317","article-title":"Identifying practices likely to have impacts on grapevine trunk disease infections: a European nursery survey.","volume":"54","author":"Gramaje","year":"2015","journal-title":"Phytopathol. Mediterr."},{"key":"B34","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1094\/PDIS-04-17-0512-FE","article-title":"Managing grapevine trunk diseases with respect to etiology and epidemiology: current strategies and future prospects.","volume":"102","author":"Gramaje","year":"2018","journal-title":"Plant Dis."},{"key":"B35","doi-asserted-by":"publisher","first-page":"395","DOI":"10.14601\/Phyto-11275","article-title":"Grapevine trunk disease fungi: their roles as latent pathogens and stress factors that favour disease develop-ment and symptom expression.","volume":"59","author":"Hrycan","year":"2020","journal-title":"Phytopathol. Mediterr."},{"key":"B36","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1023\/A:1008811501734","article-title":"Antimicrobial effects of metal ions (Ag+, Cu2+, Zn2+) in hydroxyapatite.","volume":"9","author":"Kim","year":"1998","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"B37","doi-asserted-by":"publisher","first-page":"201","DOI":"10.14601\/Phytopathol_Mediterr-2340","article-title":"Copper in plant protection: current situation and prospects.","volume":"57","author":"La Torre","year":"2018","journal-title":"Phytopathol. Mediterr."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1028","DOI":"10.1094\/PHYTO-11-12-0305-R","article-title":"A faster and a stronger defense response: one of the key elements in grapevine explaining its lower level of susceptibility to Esca?","volume":"103","author":"Lambert","year":"2013","journal-title":"Phytopathology"},{"key":"B39","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1094\/PHYTO-06-11-0165","article-title":"Colonization of Vitis spp. wood by sGFP-transformed Phaeomoniella chlamydospora, a tracheomycotic fungus involved in Esca disease.","volume":"102","author":"Landi","year":"2012","journal-title":"Phytopathology"},{"key":"B40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep17749","article-title":"Comparative transcriptome analysis of grapevine in response to copper stress.","volume":"5","author":"Leng","year":"2015","journal-title":"Sci. Rep."},{"key":"B41","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1094\/PHYTO-100-5-0424","article-title":"Early events prior to visual symptoms in the apoplectic form of grapevine Esca disease.","volume":"100","author":"Letousey","year":"2010","journal-title":"Phytopathology"},{"key":"B42","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1002\/ps.6064","article-title":"Field evaluation of biocontrol agents against black-foot and Petri diseases of grapevine.","volume":"77","author":"Mart\u00ednez-Diz","year":"2021","journal-title":"Pest Manag. Sci."},{"key":"B43","doi-asserted-by":"publisher","first-page":"232","DOI":"10.14601\/Phytopathol_Mediterr-1557","article-title":"The control of Esca: status and perspectives.","volume":"39","author":"Mazullo","year":"2000","journal-title":"Phytopathol. Mediterr."},{"key":"B44","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1071\/AP08090","article-title":"Reporter gene transformation of the trunk disease pathogen Phaeomoniella chlamydospora and biological control agent Trichoderma harzianum.","volume":"38","author":"McLean","year":"2009","journal-title":"Austr. Plant. Pathol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"453","DOI":"10.14601\/Phyto-11169","article-title":"Fungal pathogens associated with grapevine trunk diseases in young vineyards in Sicily.","volume":"59","author":"Mondello","year":"2020","journal-title":"Phytopathol. Mediterr."},{"key":"B46","doi-asserted-by":"publisher","first-page":"369","DOI":"10.14601\/Phytopathol_Mediterr-23942","article-title":"Management of grapevine trunk diseases: knowledge transfer, current strategies and innovative strategies adopted in Europe.","volume":"57","author":"Mondello","year":"","journal-title":"Phytopathol. Mediterr."},{"key":"B47","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1094\/PDIS-08-17-1181-FE","article-title":"Grapevine trunk diseases: a review of fifteen years of trials for their control with chemicals and biocontrol agents.","volume":"102","author":"Mondello","year":"","journal-title":"Plant Dis."},{"key":"B48","doi-asserted-by":"publisher","first-page":"S46","DOI":"10.14601\/Phytopathol_Mediterr-8643","article-title":"Histo-pathology study of the growth of Trichoderma harzianum, Phaeomoniella chlamydospora and Eutypa lata on grapevine pruning wounds.","volume":"50","author":"Mutawila","year":"2011","journal-title":"Phytopathol Mediterr."},{"key":"B49","author":"Oksanen","year":"2017","journal-title":"Vegan: Community Ecology Package. R Package Version 2.4-3."},{"key":"B50","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1007\/s10526-016-9723-6","article-title":"Trichoderma atroviride SC1 prevents Phaeomoniella chlamydospora and Phaeoacremonium aleophilum infection of grapevine plants during the grafting process in nurseries.","volume":"61","author":"Pertot","year":"2016","journal-title":"BioControl"},{"key":"B51","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1007\/s11120-012-9719-8","article-title":"Fungicide impacts on photosynthesis in crop plants.","volume":"111","author":"Petit","year":"2012","journal-title":"Photosynth. Res."},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126851","article-title":"Deciphering the niches of colonization of Vitis vinifera L. by the Esca-associated fungus Phaeoacremonium aleophilum using a gfp marked strain and cutting systems.","volume":"10","author":"Pierron","year":"2015","journal-title":"PLoS One"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00268","article-title":"Variations in early response of grapevine wood depending on wound and inoculation combinations with Phaeoacremonium aleophilum and Phaeomoniella chlamydospora.","volume":"7","author":"Pierron","year":"2016","journal-title":"Front. Plant Sci."},{"key":"B54","doi-asserted-by":"publisher","first-page":"407","DOI":"10.14601\/Phytopathol_Mediterr-22964","article-title":"Fungi associated with grapevine trunk diseases in nursery-produced Vitis vinifera plants.","volume":"57","author":"Pintos","year":"2018","journal-title":"Phytopathol. Mediterr."},{"key":"B55","year":"2020","journal-title":"R: A Language and Environment for Statistical Computing."},{"key":"B56","doi-asserted-by":"publisher","first-page":"916","DOI":"10.1094\/PHYTO-09-18-0349-RVW","article-title":"Vitis methods to understand and develop strategies for diagnosis and sustainable control of grapevine trunk diseases.","volume":"109","author":"Reis","year":"2019","journal-title":"Phytopathology"},{"key":"B57","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.cropro.2018.10.016","article-title":"Towards a preventive and\/or curative treatment of Esca in grapevine trunk disease: general basis in the elaboration of treatments to control plant pathogen attacks.","volume":"119","author":"Roblin","year":"2019","journal-title":"Crop Prot."},{"key":"B58","author":"Roveri","year":"2016","journal-title":"Particule d\u2019hydroxyapatite, Compositions \u00e0 base de cette Particule, Utilisations Comme Vecteur de Mol\u00e9cules Bioactives."},{"key":"B59","doi-asserted-by":"publisher","first-page":"107","DOI":"10.2147\/NSA.S9038","article-title":"Evolving application of biomimetic nanostructured hydroxyapatite.","volume":"3","author":"Roveri","year":"2010","journal-title":"Nanotechnol. Sci. Appl."},{"key":"B60","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.jplph.2019.05.010","article-title":"Sodium arsenite effect on Vitis vinifera L. physiology.","volume":"238","author":"Songy","year":"2019","journal-title":"J. Plant Physiol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"393","DOI":"10.3390\/ijms18020393","article-title":"Defense responses in grapevine (cv. Mourv\u00e8dre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and their relationship with flowering.","volume":"18","author":"Spagnolo","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"B62","doi-asserted-by":"publisher","first-page":"1638","DOI":"10.1111\/j.1151-2916.1993.tb03957.x","article-title":"Cation-exchange characteristics of sintered hydroxyapatite in the strongly acidic region.","volume":"76","author":"Suzuki","year":"1993","journal-title":"J. Am. Ceram. Soc."},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00025","article-title":"Bacillus subtilis PTA-271 counteracts Botryosphaeria dieback in grapevine, triggering immune responses and detoxification of fungal phytotoxins.","volume":"10","author":"Trotel-Aziz","year":"2019","journal-title":"Front. Plant Sci."},{"key":"B64","doi-asserted-by":"publisher","first-page":"37","DOI":"10.4454\/jpp.v91i1.622","article-title":"Developmental and ultrastructural features of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum in relation to xylem degradation in Esca disease of the grapevine.","volume":"91","author":"Valtaud","year":"2009","journal-title":"J. Plant Pathol."},{"key":"B65","doi-asserted-by":"publisher","first-page":"384","DOI":"10.14601\/Phytopathol_Mediterr-22772","article-title":"A protocol for the management of grapevine rootstock mother vines to reduce latent infections by grapevine trunk pathogens in cuttings.","volume":"57","author":"Waite","year":"2018","journal-title":"Phytopathol. Mediterr."},{"key":"B66","doi-asserted-by":"publisher","first-page":"3838","DOI":"10.1002\/ps.5935","article-title":"Evaluating the combined effect of a systemic phenylpyrrole fungicide and the plant growth-promoting rhizobacteria Paraburkholderia phytofirmans (strain PsJN:gfp2x) against the grapevine trunk pathogen Neofusicoccum parvum.","volume":"76","author":"Wu","year":"2020","journal-title":"Pest Manag. Sci."},{"key":"B67","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1590\/S1677-04202005000100012","article-title":"Copper in plants.","volume":"17","author":"Yruela","year":"2005","journal-title":"Braz. J. Plant. Physiol."}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2021.649694\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T06:09:13Z","timestamp":1615788553000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2021.649694\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,15]]},"references-count":67,"alternative-id":["10.3389\/fpls.2021.649694"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2021.649694","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,15]]},"article-number":"649694"}}