{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:53:21Z","timestamp":1787061201741,"version":"build-2736575974"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>\n                    Peach bacterial spot caused by\n                    <jats:italic>Xanthomonas arboricola<\/jats:italic>\n                    pv.\n                    <jats:italic>pruni<\/jats:italic>\n                    (\n                    <jats:italic>Xap<\/jats:italic>\n                    ) is a devastating disease worldwide and frequently causes massive economic losses. In recent years, it has become a pandemic outbreak in most peach production areas of China, especially on precocious peaches in the middle reach of the Yangtze River. Rapid, user-friendly detection is extremely important to make the correct diagnosis and develop suitable control strategies. In this study, we described a recombinase polymerase amplification (RPA)\/Cas12a-based system that combines RPA and CRISPR\/Cas12a for\n                    <jats:italic>Xap<\/jats:italic>\n                    identification. A total of three crRNAs were designed to target a highly conserved ABC transporter ATP-binding protein-encoding gene\n                    <jats:italic>ftsX<\/jats:italic>\n                    to make specific detection of\n                    <jats:italic>Xap<\/jats:italic>\n                    . Results showed that crRNA 2 and crRNA 3 could get consistent detection for\n                    <jats:italic>Xap<\/jats:italic>\n                    . To realize the visualization of detection results, we additionally introduced FQ-reporter and FB-reporter. The developed method was highly sensitive and could detect as low as 10\n                    <jats:sup>\u201318<\/jats:sup>\n                    M\n                    <jats:italic>Xap<\/jats:italic>\n                    gDNA with a mini-UV torch, corresponding to 1.63 copies\/\u03bcl or 8.855 fg\/\u03bcl gDNA of\n                    <jats:italic>Xap<\/jats:italic>\n                    , while with lateral flow strips, the sensitivity was 10\n                    <jats:sup>\u201317<\/jats:sup>\n                    M. In addition, this method could specifically detect\n                    <jats:italic>Xap<\/jats:italic>\n                    from other closely related bacteria or pathogens associated with peach diseases. Furthermore, this method could make correct identification for\n                    <jats:italic>Xap<\/jats:italic>\n                    with crude DNA using NaOH-based extraction (3 min) directly from diseased peach samples. Considering that the developed method could get results within 2 h and could be performed at 37\u00b0C (body temperature), it is promising to be applied for\n                    <jats:italic>Xap<\/jats:italic>\n                    diagnosis and monitoring in fields.\n                  <\/jats:p>","DOI":"10.3389\/fpls.2021.740177","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T08:53:02Z","timestamp":1638262382000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":30,"title":["Recombinase Polymerase Amplification\/Cas12a-Based Identification of Xanthomonas arboricola pv. pruni on Peach"],"prefix":"10.3389","volume":"12","author":[{"given":"Mei","family":"Luo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan-Zhu","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Xiao","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Xi","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,11,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2830","DOI":"10.3389\/fmicb.2019.02830","article-title":"Cas12a-based on-site and rapid nucleic acid detection of African swine fever.","volume":"10","author":"Bai","year":"2019","journal-title":"Front. 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