{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T17:40:21Z","timestamp":1775410821730,"version":"3.50.1"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T00:00:00Z","timestamp":1637107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30900969"],"award-info":[{"award-number":["30900969"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Seedless fruits are favorable in the market because of their ease of manipulation. Sucrose transporters (SUTs or SUCs) are essential for carbohydrate metabolism in plants. Whether SUTs participate directly in causing stenospermocarpy, thereby increasing fruit quality, remains unclear. Three <jats:italic>SUTs<\/jats:italic>, namely, <jats:italic>VvSUC11<\/jats:italic>, <jats:italic>VvSUC12<\/jats:italic>, and <jats:italic>VvSUC27<\/jats:italic> from <jats:italic>Vitis vinifera<\/jats:italic>, were characterized and ectopic expression in tomatoes. <jats:italic>VvSUC11<\/jats:italic>- and <jats:italic>VvSUC12<\/jats:italic>-overexpressing lines had similar flower and fruit phenotypes compared with those of the wild type. <jats:italic>VvSUC27<\/jats:italic>-overexpressing lines produced longer petals and pistils, an abnormal stigma, much less and shrunken pollen, and firmer seedless fruits. Moreover, produced fruits from all <jats:italic>VvSUC<\/jats:italic>-overexpressing lines had a higher soluble solid content and sugar concentration. Transcriptomic analysis revealed more genes associated with carbohydrate metabolism and sugar transport and showed downregulation of auxin- and ethylene-related signaling pathways during early fruit development in <jats:italic>VvSUC27<\/jats:italic>-overexpressing lines relative to that of the wild type. Our findings demonstrated that stenospermocarpy can be induced by overexpression of <jats:italic>VvSUC27<\/jats:italic> through a consequential reduction in nutrient delivery to pollen at anthesis, with a subsequent downregulation of the genes involved in carbohydrate metabolism and hormone signaling. These commercially desirable results provide a new strategy for bioengineering stenospermocarpy in tomatoes and in other fruit plants.<\/jats:p>","DOI":"10.3389\/fpls.2021.759047","type":"journal-article","created":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T05:01:18Z","timestamp":1637125278000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits"],"prefix":"10.3389","volume":"12","author":[{"given":"Yumeng","family":"Cai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenrui","family":"Tu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhefang","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjie","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyong","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yali","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1741","DOI":"10.1186\/1471-2229-10-245","article-title":"The Vitis vinifera sugar transporter gene family: phylogenetic overview and macroarray expression profiling","volume":"10","author":"Afoufa-Bastien","year":"2010","journal-title":"BMC Plant Biol."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1016\/j.mex.2018.05.014","article-title":"Quantification of sugars and organic acids in tomato fruits","volume":"5","author":"Agius","year":"2018","journal-title":"Methods"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1105\/tpc.12.7.1153","article-title":"SUT2, a putative sucrose sensor in sieve elements","volume":"12","author":"Barker","year":"2000","journal-title":"Plant Cell"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1104\/pp.118.1.59","article-title":"The H+-sucrose cotransporter NtSUT1 is essential for sugar export from tobacco leaves","volume":"118","author":"Burkle","year":"1998","journal-title":"Plant Physiol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.3389\/fpls.2017.01069","article-title":"Overexpression of a grapevine sucrose transporter (VvSUC27) in tobacco improves plant growth rate in the presence of sucrose In vitro","volume":"8","author":"Cai","year":"2017","journal-title":"Front. 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