{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T02:15:14Z","timestamp":1771380914717,"version":"3.50.1"},"reference-count":71,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T00:00:00Z","timestamp":1664928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Long non-coding RNAs (lncRNAs) are RNA molecules with functions independent of any protein-coding potential. A whole transcriptome (RNA-seq) study of Arabidopsis shoots under iron sufficient and deficient conditions was carried out to determine the genes that are iron-regulated in the shoots. We identified two previously unannotated transcripts on chromosome 1 that are significantly iron-regulated. We have called this iron-regulated lncRNA, <jats:italic>CAN OF SPINACH<\/jats:italic> (<jats:italic>COS<\/jats:italic>). <jats:italic>cos<\/jats:italic> mutants have altered iron levels in leaves and seeds. Despite the low iron levels in the leaves, <jats:italic>cos<\/jats:italic> mutants have higher chlorophyll levels than WT plants. Moreover, <jats:italic>cos<\/jats:italic> mutants have abnormal development during iron deficiency. Roots of <jats:italic>cos<\/jats:italic> mutants are longer than those of WT plants, when grown on iron deficient medium. In addition, <jats:italic>cos<\/jats:italic> mutant plants accumulate singlet oxygen during iron deficiency. The mechanism through which <jats:italic>COS<\/jats:italic> affects iron deficiency responses is unclear, but small regions of sequence similarity to several genes involved in iron deficiency responses occur in <jats:italic>COS<\/jats:italic>, and small RNAs from these regions have been detected. We hypothesize that <jats:italic>COS<\/jats:italic> is required for normal adaptation to iron deficiency conditions.<\/jats:p>","DOI":"10.3389\/fpls.2022.1005020","type":"journal-article","created":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T08:05:30Z","timestamp":1664957130000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["CAN OF SPINACH, a novel long non-coding RNA, affects iron deficiency responses in Arabidopsis thaliana"],"prefix":"10.3389","volume":"13","author":[{"given":"Ahmet","family":"Bakirbas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elsbeth L.","family":"Walker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,10,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"W537","DOI":"10.1093\/nar\/gky379","article-title":"The galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update","volume":"46","author":"Afgan","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2024918118","article-title":"Photoprotection during iron deficiency is mediated by the bHLH transcription factors PYE and ILR3","volume":"118","author":"Akmakjian","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B3","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1261\/rna.035279.112","article-title":"ShortStack: Comprehensive annotation and quantification of small RNA genes","volume":"19","author":"Axtell","year":"2013","journal-title":"RNA"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1104\/pp.18.00478","article-title":"Vacuolar iron stores gated by NRAMP3 and NRAMP4 are the primary source of iron in germinating seeds","volume":"177","author":"Bastow","year":"2018","journal-title":"Plant Physiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: A practical and powerful approach to multiple testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. R. Stat. Soc Ser. B"},{"key":"B6","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.mib.2013.07.019","article-title":"Iron sparing and recycling in a compartmentalized cell","volume":"16","author":"Blaby-Haas","year":"2013","journal-title":"Curr. Opin. Microbiol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.650926","article-title":"From \u201cDark matter\u201d to \u201cStar\u201d: Insight into the regulation mechanisms of plant functional long non-coding RNAs","volume":"12","author":"Chen","year":"2021","journal-title":"Front. Plant Sci."},{"key":"B9","doi-asserted-by":"publisher","first-page":"3400","DOI":"10.1105\/tpc.104.024315","article-title":"The essential basic helix-Loop-Helix protein FIT1 is required for the iron deficiency response","volume":"16","author":"Colangelo","year":"2004","journal-title":"Plant Cell"},{"key":"B10","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1105\/tpc.001263","article-title":"Expression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulation","volume":"14","author":"Connolly","year":"2002","journal-title":"Plant Cell"},{"key":"B11","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1039\/c7mt00136c","article-title":"Iron homeostasis in plants-a brief overview","volume":"9","author":"Connorton","year":"2017","journal-title":"Metallomics"},{"key":"B12","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1038\/35053080","article-title":"Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake","volume":"409","author":"Curie","year":"2001","journal-title":"Nature"},{"key":"B13","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1042\/bj3180067","article-title":"Characterization of a ferritin mRNA from arabidopsis thaliana accumulated in response to iron through an oxidative pathway independent of abscisic acid","volume":"318","author":"Gaymard","year":"1996","journal-title":"Biochem. J."},{"key":"B14","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1007\/BF00192543","article-title":"Photoinhibition and light-dependent turnover of the D1 reaction-centre polypeptide of photosystem II are enhanced by mineral-stress conditions","volume":"193","author":"Godde","year":"1994","journal-title":"Planta"},{"key":"B15","doi-asserted-by":"publisher","first-page":"2133","DOI":"10.1104\/PP.17.01657","article-title":"The long intergenic noncoding RNA (LincRNA) landscape of the soybean genome","volume":"176","author":"Golicz","year":"2018","journal-title":"Plant Physiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1038\/s41477-018-0266-y","article-title":"IRON MAN is a ubiquitous family of peptides that control iron transport in plants","volume":"4","author":"Grillet","year":"2018","journal-title":"Nat. Plants"},{"key":"B17","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1104\/pp.110.1.329","article-title":"Shoot-to-Root signal transmission regulates root Fe(III) reductase activity in the dgl mutant of pea","volume":"110","author":"Grusak","year":"1996","journal-title":"Plant Physiol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1093\/ACPROF:OSO\/9780198717478.003.0007","article-title":"Reactive species can pose special problems needing special solutions: Some examples","author":"Halliwell","year":"2015","journal-title":"Free Radicals Biol. Med."},{"key":"B19","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1104\/pp.17.01497","article-title":"A program for iron economy during deficiency targets specific fe proteins","volume":"176","author":"Hantzis","year":"2018","journal-title":"Plant Physiol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"W12","DOI":"10.1093\/NAR\/GKX428","article-title":"CPC2: a fast and accurate coding potential calculator based on sequence intrinsic features","volume":"45","author":"Kang","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"B21","doi-asserted-by":"publisher","first-page":"2263","DOI":"10.1111\/pce.13192","article-title":"Changes in iron availability in arabidopsis are rapidly sensed in the leaf vasculature and impaired sensing leads to opposite transcriptional programs in leaves and roots","volume":"41","author":"Khan","year":"2018","journal-title":"Plant Cell Environ."},{"key":"B22","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"B23","doi-asserted-by":"publisher","first-page":"1616","DOI":"10.1104\/PP.15.02026","article-title":"Singlet oxygen-induced membrane disruption and serpin-protease balance in vacuolar-driven cell death","volume":"171","author":"Koh","year":"2016","journal-title":"Plant Physiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1093\/jxb\/erh237","article-title":"Singlet oxygen production in photosynthesis","volume":"56","author":"Krieger-Liszkay","year":"2005","journal-title":"J. Exp. Bot. (J Exp. Bot)"},{"key":"B25","doi-asserted-by":"publisher","first-page":"1748","DOI":"10.1039\/d0mt00175a","article-title":"Iron deficiency and the loss of chloroplast iron-sulfur cluster assembly trigger distinct transcriptome changes in Arabidopsis rosettes","volume":"12","author":"Kroh","year":"2020","journal-title":"Metallomics"},{"key":"B26","doi-asserted-by":"publisher","first-page":"1254","DOI":"10.1104\/pp.17.00821","article-title":"Iron-nicotianamine transporters are required for proper long distance iron signaling","volume":"175","author":"Kumar","year":"2017","journal-title":"Plant Physiol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"100349","DOI":"10.1016\/j.xplc.2022.100349","article-title":"Iron uptake, signaling, and sensing in plants","volume":"3","author":"Liang","year":"2022","journal-title":"Plant Commun."},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.930049","article-title":"The iron deficiency-regulated small protein effector FEP3\/IRON MAN1 modulates interaction of BRUTUS-LIKE1 with bHLH subgroup IVc and POPEYE transcription factors","volume":"13","author":"Lichtblau","year":"2022","journal-title":"Front. Plant Sci."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2109063118","article-title":"IRON MAN interacts with BRUTUS to maintain iron homeostasis in arabidopsis","volume":"118","author":"Li","year":"2021","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B30","doi-asserted-by":"publisher","first-page":"3059","DOI":"10.1105\/TPC.20.00335","article-title":"PhasiRNAs in plants: Their biogenesis, genic sources, and roles in stress responses, development, and reproduction","volume":"32","author":"Liu","year":"2020","journal-title":"Plant Cell"},{"key":"B31","doi-asserted-by":"publisher","first-page":"2478","DOI":"10.1104\/pp.15.01827","article-title":"Two bHLH transcription factors, bHLH34 and bHLH104, regulate iron homeostasis in Arabidopsis thaliana","volume":"170","author":"Li","year":"2016","journal-title":"Plant Physiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"2219","DOI":"10.1105\/tpc.110.074096","article-title":"The bHLH transcription factor POPEYE regulates response to iron deficiency in arabidopsis roots","volume":"22","author":"Long","year":"2010","journal-title":"Plant Cell Online"},{"key":"B33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet.journal"},{"key":"B35","doi-asserted-by":"publisher","first-page":"373","DOI":"10.4161\/rna.28725","article-title":"Long non-coding RNA: A novel endogenous source for the generation of dicer-like 1-dependent small RNAs in arabidopsis thaliana","volume":"11","author":"Ma","year":"2014","journal-title":"RNA Biol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1093\/mp\/ssu067","article-title":"OPT3 is a component of the iron-signaling network between leaves and roots and misregulation of OPT3 leads to an over-accumulation of cadmium in seeds","volume":"7","author":"Mendoza-C\u00f3zatl","year":"2014","journal-title":"Mol. Plant"},{"key":"B37","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1038\/s41596-019-0128-8","article-title":"Protocol update for large-scale genome and gene function analysis with the PANTHER classification system (v.14.0)","volume":"14","author":"Mi","year":"2019","journal-title":"Nat. Protoc."},{"key":"B38","doi-asserted-by":"publisher","first-page":"D412","DOI":"10.1093\/NAR\/GKAA913","article-title":"Pfam: The protein families database in 2021","volume":"49","author":"Mistry","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1400","DOI":"10.1016\/J.JPLPH.2013.05.004","article-title":"How does iron deficiency disrupt the electron flow in photosystem I of lettuce leaves","volume":"170","author":"Msilini","year":"2013","journal-title":"J. Plant Physiol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-021-27548-2","article-title":"Interdependent iron and phosphorus availability controls photosynthesis through retrograde signaling","volume":"12","author":"Nam","year":"2021","journal-title":"Nat. Commun."},{"key":"B41","doi-asserted-by":"publisher","first-page":"10911","DOI":"10.1038\/s41598-017-11171-7","article-title":"Dissection of iron signaling and iron accumulation by overexpression of subgroup ib bHLH039 protein","volume":"7","author":"Naranjo-Arcos","year":"2017","journal-title":"Sci. Rep."},{"key":"B42","doi-asserted-by":"publisher","first-page":"2139","DOI":"10.1105\/tpc.112.097634","article-title":"Characterization of arabidopsis NEET reveals an ancient role for NEET proteins in iron metabolism","volume":"24","author":"Nechushtai","year":"2012","journal-title":"Plant Cell"},{"key":"B43","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1093\/plphys\/kiab350","article-title":"Impact of the SnRK1 protein kinase on sucrose homeostasis and the transcriptome during the diel cycle","volume":"187","author":"Peixoto","year":"2021","journal-title":"Plant Physiol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"B45","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1111\/j.1365-313X.2008.03698.x","article-title":"Ferritins control interaction between iron homeostasis and oxidative stress in arabidopsis","volume":"57","author":"Ravet","year":"2009","journal-title":"Plant J."},{"key":"B46","doi-asserted-by":"publisher","first-page":"858","DOI":"10.1038\/nature02353","article-title":"OXI1 kinase is necessary for oxidative burst-mediated signalling in arabidopsis","volume":"427","author":"Rentel","year":"2004","journal-title":"Nat"},{"key":"B47","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1038\/17800","article-title":"A ferric-chelate reductase for iron uptake from soils","volume":"397","author":"Robinson","year":"1999","journal-title":"Nature"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2013.00276","article-title":"The transcriptional response of arabidopsis leaves to fe deficiency","volume":"4","author":"Rodr\u00edguez-Celma","year":"2013","journal-title":"Front. Plant Sci."},{"key":"B49","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"B50","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.ymeth.2013.10.004","article-title":"Identification and annotation of small RNA genes using ShortStack","volume":"67","author":"Shahid","year":"2014","journal-title":"Methods"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1104\/PP.65.1.121","article-title":"Limiting factors in photosynthesis: II. IRON STRESS DIMINISHES PHOTOCHEMICAL CAPACITY BY REDUCING THE NUMBER OF PHOTOSYNTHETIC UNITS 1, 2","volume":"65","author":"Spiller","year":"1980","journal-title":"Plant Physiol."},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1199\/tab.0145","article-title":"Tetrapyrrole metabolism in arabidopsis thaliana","volume":"9","author":"Tanaka","year":"2011","journal-title":"Arab. B."},{"key":"B53","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1016\/J.ENVEXPBOT.2013.03.006","article-title":"Iron deprivation-induced reactive oxygen species generation leads to non-autolytic PCD in brassica napus leaves","volume":"91","author":"Tewari","year":"2013","journal-title":"Environ. Exp. Bot."},{"key":"B54","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1002\/PRO.4218","article-title":"PANTHER: Making genome-scale phylogenetics accessible to all","volume":"31","author":"Thomas","year":"2022","journal-title":"Protein Sci."},{"key":"B55","doi-asserted-by":"publisher","first-page":"960","DOI":"10.1104\/PP.108.125690","article-title":"Singlet oxygen is the major reactive oxygen species involved in photooxidative damage to plants","volume":"148","author":"Triantaphylid\u00e8s","year":"2008","journal-title":"Plant Physiol."},{"key":"B56","doi-asserted-by":"publisher","first-page":"3921","DOI":"10.1105\/tpc.112.102491","article-title":"Systems and Trans -system level analysis identifies conserved iron deficiency responses in the plant lineage","volume":"24","author":"Urzica","year":"2012","journal-title":"Plant Cell"},{"key":"B57","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1105\/tpc.001388","article-title":"IRT1, an arabidopsis transporter essential for iron uptake from the soil and for plant growth","volume":"14","author":"Vert","year":"2002","journal-title":"Plant Cell"},{"key":"B58","first-page":"133","article-title":"Long noncoding RNAs in plants","volume-title":"Advances in experimental medicine and biology","author":"Wang","year":"2017"},{"key":"B59","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1007\/s00425-007-0535-x","article-title":"Iron deficiency-mediated stress regulation of four subgroup ib BHLH genes in arabidopsis thaliana","volume":"226","author":"Wang","year":"2007","journal-title":"Planta"},{"key":"B60","first-page":"468","article-title":"\u201cIron: Properties and Determination","volume-title":"Encyclopedia of Food and Health","author":"Ward","year":"2015"},{"key":"B61","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.1104\/pp.106.082586","article-title":"Mutations in arabidopsis yellow stripe-like1 and yellow stripe-like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds","volume":"141","author":"Waters","year":"2006","journal-title":"Plant Physiol."},{"key":"B62","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1146\/annurev-arplant-093020-035446","article-title":"Long noncoding RNAs in plants","volume":"72","author":"Wierzbicki","year":"2021","journal-title":"Annu. Rev. Plant Biol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/J.JPLPH.2012.02.008","article-title":"Differential degradation of photosystem I subunits under iron deficiency in rice","volume":"169","author":"Yadavalli","year":"2012","journal-title":"J. Plant Physiol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1038\/cr.2008.26","article-title":"FIT interacts with AtbHLH38 and AtbHLH39 in regulating iron uptake gene expression for iron homeostasis in arabidopsis","volume":"18","author":"Yuan","year":"2008","journal-title":"Cell Res."},{"key":"B65","doi-asserted-by":"publisher","first-page":"6736","DOI":"10.1038\/s41598-022-10784-x","article-title":"Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology","volume":"12","author":"Yu","year":"2022","journal-title":"Sci. Rep."},{"key":"B66","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.1111\/TPJ.14581","article-title":"Expression of a dominant-negative AtNEET-H89C protein disrupts iron\u2013sulfur metabolism and iron homeostasis in arabidopsis","volume":"101","author":"Zandalinas","year":"2020","journal-title":"Plant J."},{"key":"B67","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S12864-016-3478-4\/TABLES\/3","article-title":"Transcriptional and physiological analyses of fe deficiency response in maize reveal the presence of strategy I components and Fe\/P interactions","volume":"18","author":"Zanin","year":"2017","journal-title":"BMC Genomics"},{"key":"B68","doi-asserted-by":"publisher","first-page":"2249","DOI":"10.1105\/tpc.114.123737","article-title":"OPT3 is a phloem-specific iron transporter that is essential for systemic iron signaling and redistribution of iron and cadmium in arabidopsis","volume":"26","author":"Zhai","year":"2014","journal-title":"Plant Cell"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20102424","article-title":"The adaptive mechanism of plants to iron deficiency via iron uptake, transport, and homeostasis","volume":"20","author":"Zhang","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"B70","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1016\/j.molp.2020.08.001","article-title":"A comprehensive online database for exploring -20,000 public arabidopsis RNA-seq libraries","volume":"13","author":"Zhang","year":"2020","journal-title":"Mol. Plant"},{"key":"B71","doi-asserted-by":"publisher","first-page":"3619","DOI":"10.1126\/SCIADV.AAX3619","article-title":"Genome-wide analyses reveal the role of noncoding variation in complex traits during rice domestication","volume":"5","author":"Zheng","year":"2019","journal-title":"Sci. Adv."}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2022.1005020\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T08:05:34Z","timestamp":1664957134000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2022.1005020\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,5]]},"references-count":71,"alternative-id":["10.3389\/fpls.2022.1005020"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2022.1005020","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,5]]},"article-number":"1005020"}}