{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T23:22:10Z","timestamp":1774048930264,"version":"3.50.1"},"reference-count":79,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T00:00:00Z","timestamp":1644796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007822","name":"Almond Board of California","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007822","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Almond [<jats:italic>Prunus dulcis<\/jats:italic>(Mill.) D.A. Webb] is an economically important nut crop susceptible to the genetic disorder, Non-infectious Bud Failure (NBF). Despite the severity of exhibition in several prominent almond cultivars, no causal mechanism has been identified underlying NBF development. The disorder is hypothesized to be associated with differential DNA methylation patterns based on patterns of inheritance (i.e., via sexual reproduction and clonal propagation) and previous work profiling methylation in affected trees. Peach (<jats:italic>Prunus persica<\/jats:italic>L. Batsch) is a closely related species that readily hybridizes with almond; however, peach is not known to exhibit NBF. A cross between an NBF-exhibiting \u2018Carmel\u2019 cultivar and early flowering peach (\u201840A17\u2019) produced an F<jats:sub>1<\/jats:sub>where \u223c50% of progeny showed signs of NBF, including canopy die-back, erratic branching patterns (known as \u201ccrazy-top\u201d), and rough bark. In this study, whole-genome DNA methylation profiles were generated for three F<jats:sub>1<\/jats:sub>progenies exhibiting NBF and three progenies considered NBF-free. Subsequent alignment to both the almond and peach reference genomes showed an increase in genome-wide methylation levels in NBF hybrids in CG and CHG contexts compared to no-NBF hybrids when aligned to the almond genome but no difference in methylation levels when aligned to the peach genome. Significantly differentially methylated regions (DMRs) were identified by comparing methylation levels across the genome between NBF- and no-NBF hybrids in each methylation context. In total, 115,635 DMRs were identified based on alignment to the almond reference genome, and 126,800 DMRs were identified based on alignment to the peach reference genome. Nearby genes were identified as associated with the 39 most significant DMRs occurring either in the almond or peach alignments alone or occurring in both the almond and peach alignments. These DMR-associated genes include several uncharacterized proteins and transposable elements. Quantitative PCR was also performed to analyze the gene expression patterns of these identified gene targets to determine patterns of differential expression associated with differential DNA methylation. These DMR-associated genes, particularly those showing corresponding patterns of differential gene expression, represent key targets for almond breeding for future cultivars and mitigating the effects of NBF-exhibition in currently affected cultivars.<\/jats:p>","DOI":"10.3389\/fpls.2022.804145","type":"journal-article","created":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T05:37:22Z","timestamp":1644817042000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of Putative Markers of Non-infectious Bud Failure in Almond [Prunus dulcis (Mill.) D.A. Webb] Through Genome Wide DNA Methylation Profiling and Gene Expression Analysis in an Almond \u00d7 Peach Hybrid Population"],"prefix":"10.3389","volume":"13","author":[{"given":"Katherine M.","family":"D\u2019Amico-Willman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gina M.","family":"Sideli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian J.","family":"Allen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth S.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas M.","family":"Gradziel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Fresnedo-Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,2,14]]},"reference":[{"key":"B2","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/gb-2012-13-10-r87","article-title":"methylKit: a comprehensive R package for the analysis of genome-wide DNA methylation profiles.","volume":"13","author":"Akalin","year":"2012","journal-title":"Genome Biol."},{"key":"B3","author":"Andrews","year":"2010","journal-title":"FastQC: A Quality Control Tool for High Throughput Sequence Data."},{"key":"B4","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1007\/s00425-005-0184-x","article-title":"Molecular characterization of a receptor-like protein kinase gene from pine (Pinus sylvestris L.).","volume":"224","author":"\u00c1vila","year":"2006","journal-title":"Planta"},{"key":"B5","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1002\/dvg.22877","article-title":"The arabidopsis information resource: making and mining the \u201cgold standard\u201d annotated reference plant genome.","volume":"53","author":"Berardini","year":"2015","journal-title":"Plant Genome"},{"key":"B6","doi-asserted-by":"publisher","first-page":"11034","DOI":"10.3390\/ijms222011034","article-title":"Serotonin and melatonin biosynthesis in plants: genome-wide identification of the genes and their expression reveal a conserved role in stress and development.","volume":"22","author":"Bhowal","year":"2021","journal-title":"IJMS"},{"key":"B7","year":"2020","journal-title":"2019 California Almond Acreage Report."},{"key":"B8","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1093\/toxsci\/kfg138","article-title":"The long (LINEs) and the short (SINEs) of it: altered methylation as a precursor to toxicity.","volume":"75","author":"Carnell","year":"2003","journal-title":"Toxicol. Sci."},{"key":"B9","doi-asserted-by":"publisher","first-page":"e70384","DOI":"10.1371\/journal.pone.0070384","article-title":"Evolution of chloroplast J proteins.","volume":"8","author":"Chiu","year":"2013","journal-title":"PLoS One"},{"key":"B10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v034.i02","article-title":"Beta regression in R.","volume":"34","author":"Cribari-Neto","year":"2010","journal-title":"J. Stat. Softw."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.1038\/ng.3886","article-title":"High-quality de novo assembly of the apple genome and methylome dynamics of early fruit development.","volume":"49","author":"Daccord","year":"2017","journal-title":"Nat. Genet."},{"key":"B12","author":"D\u2019Amico Willman","year":"2021","journal-title":"Almond as a Model to Explore Epigenetic Signatures Associated With Aging in Perennial Plants."},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1101\/2021.02.08.430330","article-title":"Integrated analysis of the methylome and transcriptome of twin almonds (Prunus dulcis [Mill.] D.A.Webb) reveals genomic features associated with non-infectious bud failure.","author":"D\u2019Amico-Willman","year":"2021","journal-title":"bioRxiv"},{"key":"B14","first-page":"77","article-title":"Free amino acid N in noninfectious bud-failure of \u201cNonpareil\u201d almond.","volume":"11","author":"Durzan","year":"1997","journal-title":"Adv. Hortic. Sci."},{"key":"B15","doi-asserted-by":"publisher","first-page":"2150","DOI":"10.1105\/tpc.17.00010","article-title":"Subgenome dominance in an interspecific hybrid, synthetic allopolyploid, and a 140-year-old naturally established neo-allopolyploid monkeyflower.","volume":"29","author":"Edger","year":"2017","journal-title":"Plant Cell"},{"key":"B16","doi-asserted-by":"publisher","first-page":"e69","DOI":"10.1093\/nar\/gku154","article-title":"A bayesian hierarchical model to detect differentially methylated loci from single nucleotide resolution sequencing data.","volume":"42","author":"Feng","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"B17","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.21273\/HORTSCI.23.6.1050","article-title":"Search for a viroid etiology for noninfectious bud failure in almond.","volume":"23","author":"Fenton","year":"1988","journal-title":"HortScience"},{"key":"B18","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1093\/treephys\/tpx137","article-title":"Transcriptome analysis of Pinus halepensis under drought stress and during recovery.","volume":"38","author":"Fox","year":"2018","journal-title":"Tree Physiol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"42686","DOI":"10.1038\/srep42686","article-title":"Genome-wide DNA-(de)methylation is associated with noninfectious bud-failure exhibition in almond (Prunus dulcis [Mill.] D.A.Webb).","volume":"7","author":"Fresnedo-Ram\u00edrez","year":"2017","journal-title":"Sci. Rep."},{"key":"B20","doi-asserted-by":"publisher","first-page":"18810","DOI":"10.1074\/jbc.M413247200","article-title":"Cullins 3a and 3b assemble with members of the broad complex\/tramtrack\/bric-a-brac (BTB) protein family to form sssential ubiquitin-protein ligases (E3s) in arabidopsis.","volume":"280","author":"Gingerich","year":"2005","journal-title":"J. Biol. Chem."},{"key":"B21","doi-asserted-by":"publisher","first-page":"200","DOI":"10.3389\/fpls.2020.00200","article-title":"Competition of parental genomes in plant hybrids.","volume":"11","author":"Glombik","year":"2020","journal-title":"Front. Plant Sci."},{"key":"B22","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/S1360-1385(98)01232-1","article-title":"Activation of plant retrotransposons under stress conditions.","volume":"3","author":"Grandbastien","year":"1998","journal-title":"Trends Plant Sci."},{"key":"B23","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1038\/337376a0","article-title":"Tnt1, a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics.","volume":"337","author":"Grandbastien","year":"1989","journal-title":"Nature"},{"key":"B24","doi-asserted-by":"publisher","first-page":"2811","DOI":"10.1093\/bioinformatics\/btu393","article-title":"Circlize implements and enhances circular visualization in R.","volume":"30","author":"Gu","year":"2014","journal-title":"Bioinformatics"},{"key":"B25","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1186\/s13059-020-02099-9","article-title":"Contiguous and stochastic CHH methylation patterns of plant DRM2 and CMT2 revealed by single-read methylome analysis.","volume":"21","author":"Harris","year":"2020","journal-title":"Genome Biol."},{"key":"B26","doi-asserted-by":"crossref","first-page":"375","DOI":"10.21273\/JASHS.104.3.375","article-title":"High temperature induced bud-failure symptoms in vegetative buds of almond plants in growth chambers.","volume":"104","author":"Hellali","year":"1979","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"B27","doi-asserted-by":"crossref","first-page":"459","DOI":"10.21273\/JASHS.103.4.459","article-title":"Morphology of noninfectious bud failure symptoms in vegetative buds of almond (Prunus amygdalus batch).","volume":"103","author":"Hellali","year":"1978","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"B28","doi-asserted-by":"publisher","first-page":"e33816","DOI":"10.1371\/journal.pone.0033816","article-title":"The Tnt1 retrotransposon escapes silencing in tobacco, its natural host.","volume":"7","author":"Hern\u00e1ndez-Pinz\u00f3n","year":"2012","journal-title":"PLoS One"},{"key":"B29","doi-asserted-by":"publisher","first-page":"4079","DOI":"10.1038\/s41467-020-17963-2","article-title":"DNA methylation is reconfigured at the onset of reproduction in rice shoot apical meristem.","volume":"11","author":"Higo","year":"2020","journal-title":"Nat. Commun."},{"key":"B30","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1002\/bmj.200810425","article-title":"Simultaneous inference in general parametric models.","volume":"50","author":"Hothorn","year":"2008","journal-title":"Biometr. J."},{"key":"B31","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1111\/tpj.14531","article-title":"CG hypomethylation leads to complex changes in DNA methylation and transpositional burst of diverse transposable elements in callus cultures of rice.","volume":"101","author":"Hu","year":"2020","journal-title":"Plant J."},{"key":"B32","doi-asserted-by":"publisher","first-page":"110181","DOI":"10.1016\/j.plantsci.2019.110181","article-title":"A non-LTR retrotransposon activates anthocyanin biosynthesis by regulating a MYB transcription factor in Capsicum annuum.","volume":"287","author":"Jung","year":"2019","journal-title":"Plant Sci."},{"key":"B33","first-page":"12","article-title":"Noninfectious bud failure of almonds in California (1) The nature and origin.","volume":"23","author":"Kester","year":"1969","journal-title":"California Agric."},{"key":"B34","first-page":"372","article-title":"Comparative inheritance of noninfectious bud-failure (BF) in almond x almond and almond x peach progenies.","volume":"13","author":"Kester","year":"1978","journal-title":"HortScience"},{"key":"B35","doi-asserted-by":"crossref","first-page":"377","DOI":"10.21273\/JASHS.103.3.377","article-title":"Variability in noninfectious bud-failure of \u201cNonpareil\u201d almond. I. location and environment.","volume":"103","author":"Kester","year":"1978","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"B36","doi-asserted-by":"crossref","first-page":"492","DOI":"10.21273\/JASHS.95.4.492","article-title":"Non-infectious bud-failure from breeding programs of almond (Prunus amygdalas batsch).","volume":"95","author":"Kester","year":"1970","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"B37","doi-asserted-by":"crossref","first-page":"244","DOI":"10.21273\/JASHS.129.2.0244","article-title":"Noninfectious bud failure in \u2018carmel\u2019 almond: I. pattern of development in vegetative progeny trees.","volume":"129","author":"Kester","year":"2004","journal-title":"J. Amer. Soc. Hort. Sci."},{"key":"B38","author":"Krueger","year":"2016","journal-title":"Taking Appropriate QC Measures or RBBS-Type or Other Seq-Applications with TrimGalore!."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1093\/bioinformatics\/btr167","article-title":"Bismark: a flexible aligner and methylation caller for bisulfite-seq applications.","volume":"27","author":"Krueger","year":"2011","journal-title":"Bioinformatics"},{"key":"B40","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/S0014-5793(98)01428-8","article-title":"Expression and characterization of PP7, a novel plant protein Ser\/Thr phosphatase distantly related to RdgC\/PPEF and PP5.","volume":"440","author":"Kutuzov","year":"1998","journal-title":"FEBS Lett."},{"key":"B41","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.gde.2018.04.002","article-title":"Transposable elements: all mobile, all different, some stress responsive, some adaptive?","volume":"49","author":"Lanciano","year":"2018","journal-title":"Curr. Opin. Genet. Dev."},{"key":"B42","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1016\/j.cell.2008.03.029","article-title":"Highly integrated single-base resolution maps of the epigenome in arabidopsis.","volume":"133","author":"Lister","year":"2008","journal-title":"Cell"},{"key":"B43","doi-asserted-by":"publisher","first-page":"702874","DOI":"10.3389\/fpls.2021.702874","article-title":"Melatonin relieves ozone stress in grape leaves by inhibiting ethylene biosynthesis.","volume":"12","author":"Liu","year":"2021","journal-title":"Front. Plant Sci."},{"key":"B44","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1111\/j.1365-3040.2006.01613.x","article-title":"Calmodulin-binding protein phosphatase PP7 is involved in thermotolerance in arabidopsis.","volume":"30","author":"Liu","year":"2007","journal-title":"Plant Cell Environ."},{"key":"B45","doi-asserted-by":"publisher","first-page":"W636","DOI":"10.1093\/nar\/gkz268","article-title":"The EMBL-EBI search and sequence analysis tools APIs in 2019.","volume":"47","author":"Madeira","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B46","doi-asserted-by":"publisher","first-page":"evab144","DOI":"10.1093\/gbe\/evab144","article-title":"CHH methylation islands: a nonconserved feature of grass genomes that is positively associated with transposable elements but negatively associated with gene-body methylation.","volume":"13","author":"Martin","year":"2021","journal-title":"Genome Biol. Evol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"R64","DOI":"10.1186\/gb-2009-10-6-r64","article-title":"A novel and universal method for microRNA RT-qPCR data normalization.","volume":"10","author":"Mestdagh","year":"2009","journal-title":"Genome Biol."},{"key":"B48","author":"Micke","year":"1996","journal-title":"Almond Production Manual."},{"key":"B49","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1038\/s41477-020-0672-9","article-title":"A single gene underlies the dynamic evolution of poplar sex determination.","volume":"6","author":"M\u00fcller","year":"2020","journal-title":"Nat. Plants"},{"key":"B50","doi-asserted-by":"publisher","first-page":"1136","DOI":"10.1016\/j.phytochem.2011.02.029","article-title":"Autophosphorylation profiling of arabidopsis protein kinases using the cell-free system.","volume":"72","author":"Nemoto","year":"2011","journal-title":"Phytochemistry"},{"key":"B51","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1007\/s004380050943","article-title":"Non-LTR retrotransposons (LINEs) as ubiquitous components of plant genomes.","volume":"261","author":"Noma","year":"1999","journal-title":"Mol. Genet. Genom."},{"key":"B52","year":"1987"},{"key":"B53","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.1093\/bioinformatics\/btw026","article-title":"Differential methylation analysis for BS-seq data under general experimental design.","volume":"32","author":"Park","year":"2016","journal-title":"Bioinformatics"},{"key":"B54","year":"2021","journal-title":"R: A Language and Environment for Statistical Computing."},{"key":"B55","doi-asserted-by":"publisher","first-page":"10431","DOI":"10.1093\/nar\/gkab828","article-title":"Genomic impact of stress-induced transposable element mobility in Arabidopsis.","volume":"49","author":"Roquis","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"B56","year":"2020","journal-title":"JMP<sup>\u00ae<\/sup>, Version 15.2.1 Pro. 1989\u20132021"},{"key":"B57","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1023\/A:1006212929794","article-title":"LINEs, SINEs and repetitive DNA: non-LTR retrotransposons in plant genomes.","volume":"40","author":"Schmidt","year":"1999","journal-title":"Plant Mol. Biol."},{"key":"B58","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1159\/000511119","article-title":"Chromosomal locations of a non-LTR retrotransposon, menolird18, in Cucumis melo and Cucumis sativus, and its implication on genome evolution of cucumis species.","volume":"160","author":"Setiawan","year":"2020","journal-title":"Cytogen. Genome Res."},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1079\/9781780643540.0000","author":"Socias","year":"2017","journal-title":"Almonds: Botany, Production and Uses."},{"key":"B60","doi-asserted-by":"publisher","first-page":"e1002674","DOI":"10.1371\/journal.pgen.1002674","article-title":"Hypersensitive to red and blue 1 and its modification by protein phosphatase 7 are implicated in the control of arabidopsis stomatal aperture.","volume":"8","author":"Sun","year":"2012","journal-title":"PLoS Genet."},{"key":"B61","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.tplants.2005.03.009","article-title":"Insertional mutagenesis: a swiss army knife for functional genomics of Medicago truncatula.","volume":"10","author":"Tadege","year":"2005","journal-title":"Trends Plant Sci."},{"key":"B62","doi-asserted-by":"publisher","first-page":"D480","DOI":"10.1093\/nar\/gkaa1100","article-title":"UniProt: the universal protein knowledgebase in 2021.","volume":"49","author":"UniProt Consortium, Bateman","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"B63","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/bs.abr.2018.09.001","article-title":"Transposable elements as tool for crop improvement.","volume":"88","author":"Thieme","year":"2018","journal-title":"Adv. Bot. Res."},{"key":"B64","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1007\/978-1-0716-1134-0_8","article-title":"Identification of active transposable elements in plants: the mobilome-seq approach","author":"Thieme","year":"2021","journal-title":"Plant Transposable Elements Methods in Molecular Biology"},{"key":"B65","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1186\/1471-2199-10-71","article-title":"Selection of reliable reference genes for gene expression studies in peach using real-time PCR.","volume":"10","author":"Tong","year":"2009","journal-title":"BMC Mol. Biol."},{"key":"B66","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1186\/s13007-020-00652-y","article-title":"SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species.","volume":"16","author":"Vilanova","year":"2020","journal-title":"Plant Methods"},{"key":"B67","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1007\/s11295-014-0733-1","article-title":"Stress-induced transcriptional activation of retrotransposon-like sequences in the Scots pine (Pinus sylvestris L.) genome.","volume":"10","author":"Voronova","year":"2014","journal-title":"Tree Genet. Genomes"},{"key":"B68","doi-asserted-by":"publisher","first-page":"4983","DOI":"10.1093\/jxb\/ers176","article-title":"Interhaplotypic heterogeneity and heterochromatic features may contribute to recombination suppression at the S locus in apple (malus\u00d7domestica).","volume":"63","author":"Wang","year":"2012","journal-title":"J. Exp. Bot."},{"key":"B69","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1093\/jxb\/erx473","article-title":"Phytomelatonin: a universal abiotic stress regulator.","volume":"69","author":"Wang","year":"2018","journal-title":"J. Exp. Bot."},{"key":"B70","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1038\/s41467-020-14995-6","article-title":"Transposon age and non-CG methylation.","volume":"11","author":"Wang","year":"2020","journal-title":"Nat, Commun."},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-021-02813-2","article-title":"H2S aids osmotic stress resistance by S-sulfhydration of melatonin production-related enzymes in Arabidopsis thaliana.","author":"Wang","year":"2021","journal-title":"Plant Cell Rep."},{"key":"B72","first-page":"970","article-title":"Observations on the bud-failure disorder in jordanolo, a new variety of almond.","volume":"40","author":"Wilson","year":"1950","journal-title":"Phytopathology"},{"key":"B73","first-page":"520","article-title":"Development of bud-failure in the jordanolo variety of almond.","volume":"42","author":"Wilson","year":"1952","journal-title":"Phytopathology"},{"key":"B74","doi-asserted-by":"publisher","first-page":"519","DOI":"10.3733\/hilg.v24n18p519","article-title":"The nature and development of noninfectious bud failure in almonds.","volume":"24","author":"Wilson","year":"1956","journal-title":"Hilgardia"},{"key":"B75","first-page":"60","article-title":"A bud-failure disorder in almond trees.","volume":"33","author":"Wilson","year":"1944","journal-title":"Bull. California Dep. Agric."},{"key":"B76","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1093\/biostatistics\/kxs033","article-title":"A new shrinkage estimator for dispersion improves differential expression detection in RNA-seq data.","volume":"14","author":"Wu","year":"2013","journal-title":"Biostatistics"},{"key":"B77","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1038\/s41438-021-00588-x","article-title":"Dynamic changes in transposable element and gene methylation in mulberry (Morus notabilis) in response to Botrytis cinerea.","volume":"8","author":"Xin","year":"2021","journal-title":"Hortic. Res."},{"key":"B78","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1111\/pbi.12820","article-title":"Single-base methylome analysis reveals dynamic epigenomic differences associated with water deficit in apple.","volume":"16","author":"Xu","year":"2017","journal-title":"Plant Biotechnol. J."},{"key":"B79","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1111\/jpi.12359","article-title":"Melatonin enhances thermotolerance by promoting cellular protein protection in tomato plants.","volume":"61","author":"Xu","year":"2016","journal-title":"J. Pineal Res."},{"key":"B80","doi-asserted-by":"publisher","first-page":"683228","DOI":"10.3389\/fpls.2021.683228","article-title":"Roles of endogenous melatonin in resistance to Botrytis cinerea infection in an arabidopsis model.","volume":"12","author":"Zhu","year":"2021","journal-title":"Front. Plant Sci."}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2022.804145\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,26]],"date-time":"2023-01-26T20:20:19Z","timestamp":1674764419000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2022.804145\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,14]]},"references-count":79,"alternative-id":["10.3389\/fpls.2022.804145"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2022.804145","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,14]]},"article-number":"804145"}}