{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T01:00:24Z","timestamp":1768870824564,"version":"3.49.0"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:00:00Z","timestamp":1647907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>The genotype CR60 is a spontaneous Cherry Red variant (containing granular red dapples on flue-cured leaves) of the Yunyan 87 (Y87) tobacco; it accumulates higher concentration of iron (Fe) in leaves than Y87, but the physiological differences between them remain largely unknown. We investigated the physiological and molecular mechanisms of CR60 in response to Fe deficiency under hydroponic conditions. Our results showed no significant phenotypic difference between Y87 and CR60 at optimal (40 \u03bcM) and high Fe (160 and 320 \u03bcM) concentrations. By contrast, CR60 exhibited higher tolerance to Fe deficiency (0 \u03bcM) than Y87, as shown by higher concentrations of chlorophyll in CR60 leaves after 21-day Fe-deficiency stress. Transcriptome profiling coupled with RT-PCR analyses found that the expression of <jats:italic>IRT1<\/jats:italic> and several genes associated with chlorophyll biosynthesis and photosynthesis (e.g., <jats:italic>PRO<\/jats:italic>, <jats:italic>GSA<\/jats:italic>, <jats:italic>FD1<\/jats:italic>, <jats:italic>PsbO<\/jats:italic>, and <jats:italic>PC<\/jats:italic>) was higher in CR60 than Y87. These results indicated that CR60 maintains sufficient Fe uptake, chlorophyll biosynthesis and photosynthetic rate when subjected to Fe starvation.<\/jats:p>","DOI":"10.3389\/fpls.2022.861081","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T18:49:55Z","timestamp":1648406995000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Physiological and Molecular Changes in Cherry Red Tobacco in Response to Iron Deficiency Stress"],"prefix":"10.3389","volume":"13","author":[{"given":"Fei","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Pu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyi","family":"Sui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zed","family":"Rengel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongbang","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1146\/annurev-arplant-050213-035759","article-title":"Iron cofactor assembly in plants.","volume":"65","author":"Balk","year":"2014","journal-title":"Annu. Rev. Plant Biol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.tplants.2014.07.005","article-title":"Iron nutrition, biomass production, and plant product quality.","volume":"20","author":"Briat","year":"2015","journal-title":"Trends Plant Sci."},{"key":"B3","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.tplants.2014.11.004","article-title":"Molecular mechanisms governing Arabidopsis iron uptake.","volume":"20","author":"Brumbarova","year":"2015","journal-title":"Trends Plant Sci."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.796893","article-title":"Comparative physiological and transcriptomic analyses reveal altered Fe-deficiency fesponses in tomato epimutant colorless non-ripening.","volume":"12","author":"Chen","year":"2022","journal-title":"Front. Plant Sci."},{"key":"B5","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1039\/c7mt00136c","article-title":"Iron homeostasis in plants - a brief overview.","volume":"9","author":"Connorton","year":"2017","journal-title":"Metallomics"},{"key":"B6","doi-asserted-by":"publisher","first-page":"5624","DOI":"10.1073\/pnas.93.11.5624","article-title":"A novel iron-regulated metal transporter from plants identified by functional expression in yeast.","volume":"93","author":"Eide","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B7","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1016\/j.pbi.2004.03.007","article-title":"Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport.","volume":"7","author":"Fernie","year":"2004","journal-title":"Curr. Opin. Plant Biol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1104\/pp.104.3.815","article-title":"Iron: nutritious, noxious, and not readily available.","volume":"104","author":"Guerinot","year":"1994","journal-title":"Plant Physiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1104\/pp.40.1.45","article-title":"Conversion of nicotine to nornicotine in grafts between cherry-red tobacco and related materials.","volume":"40","author":"Hall","year":"1965","journal-title":"Plant Physiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1007\/s00425-002-0920-4","article-title":"Iron uptake, trafficking and homeostasis in plants.","volume":"216","author":"Hell","year":"2003","journal-title":"Planta"},{"key":"B11","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1139\/b80-044","article-title":"Erratum: a method for the extraction of chlorophyll from leaf tissue without maceration.","volume":"58","author":"Hiscox","year":"1980","journal-title":"Can. J. Bot."},{"key":"B12","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.bbabio.2012.10.001","article-title":"Insight into the evolution of the iron oxidation pathways.","volume":"1827","author":"Ilbert","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"B13","doi-asserted-by":"publisher","first-page":"2273","DOI":"10.1016\/j.febslet.2007.04.043","article-title":"Mining Iron: iron uptake and transport in plants.","volume":"581","author":"Kim","year":"2007","journal-title":"FEBS Lett."},{"key":"B14","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1146\/annurev-arplant-042811-105522","article-title":"Iron uptake, translocation, and regulation in higher plants.","volume":"63","author":"Kobayashi","year":"2012","journal-title":"Annu. Rev. Plant Biol."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21093395","article-title":"Regulation of iron homeostasis and use in chloroplasts.","volume":"21","author":"Kroh","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.710093","article-title":"Iron deficiency leads to chlorosis through impacting chlorophyll synthesis and nitrogen metabolism in Areca catechu L.","volume":"12","author":"Li","year":"2021","journal-title":"Front. Plant Sci."},{"key":"B17","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"Feature counts: an efficient general purpose program for assigning sequence reads to genomic features.","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"B18","doi-asserted-by":"publisher","first-page":"F4.3.1","DOI":"10.1002\/0471142913.faf0403s01","article-title":"Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy.","volume":"1","author":"Lichtenthaler","year":"2001","journal-title":"Curr. Protoc. Food Anal. Chem."},{"key":"B19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/01904168409363172","article-title":"Iron chlorosis, a world wide problem, the relation of chlorophyll biosynthesis to iron.","volume":"7","author":"Miller","year":"2008","journal-title":"J. Plant Nutr."},{"key":"B20","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1038\/nmeth.1226","article-title":"Mapping and quantifying mammalian transcriptomes by RNA-Seq.","volume":"5","author":"Mortazavi","year":"2008","journal-title":"Nat. Methods"},{"key":"B21","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1007\/s11120-011-9628-2","article-title":"Inhibition of photosynthetic oxygen evolution and electron transfer from the quinone acceptor QA- to QB by iron deficiency.","volume":"107","author":"Msilini","year":"2011","journal-title":"Photosynth. Res."},{"key":"B22","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1038\/nrm1525","article-title":"The complex architecture of oxygenic photosynthesis.","volume":"5","author":"Nelson","year":"2004","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1111\/j.1365-313X.2008.03698.x","article-title":"Ferritins control interaction between iron homeostasis and oxidative stress in Arabidopsis.","volume":"57","author":"Ravet","year":"2009","journal-title":"Plant J."},{"key":"B24","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1038\/17800","article-title":"A ferric-chelate reductase for iron uptake from soils.","volume":"397","author":"Robinson","year":"1999","journal-title":"Nature"},{"key":"B25","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1104\/pp.80.1.175","article-title":"Evidence for a specific uptake system for iron phytosiderophores in roots of grasses.","volume":"80","author":"Romheld","year":"1986","journal-title":"Plant Physiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1111\/j.1469-8137.2009.02908.x","article-title":"Dissecting iron deficiency-induced proton extrusion in Arabidopsis roots.","volume":"183","author":"Santi","year":"2009","journal-title":"New Phytol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"14919","DOI":"10.1073\/pnas.0506581102","article-title":"Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase.","volume":"102","author":"Siminszky","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B28","doi-asserted-by":"publisher","first-page":"75","DOI":"10.16472\/j.chinatobacco.2021.019","article-title":"The CYP82E4 expression pattern and nicotine conversion rate in Cherry-red tobacco.","volume":"27","author":"Song","year":"2021","journal-title":"Acta Tabacaria Sin."},{"key":"B29","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1007\/978-981-10-7757-9_7","article-title":"Mitochondrial respiratory chain complexes.","volume":"87","author":"Sousa","year":"2018","journal-title":"Subcell Biochem."},{"key":"B30","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1046\/j.1365-313x.2003.01760.x","article-title":"AtNRAMP3, a multispecific vacuolar metal transporter involved in plant responses to iron deficiency.","volume":"34","author":"Thomine","year":"2003","journal-title":"Plant J."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1105\/tpc.001388","article-title":"IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth.","volume":"14","author":"Vert","year":"2002","journal-title":"Plant Cell"},{"key":"B32","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1016\/j.tplants.2018.07.005","article-title":"Iron-Requiring enzymes in the spotlight of oxygen.","volume":"23","author":"Vigani","year":"2018","journal-title":"Trends Plant Sci."},{"key":"B33","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/0003-9861(56)90145-x","article-title":"Conversion of nicotine to nornicotine in Cherry Red tobacco during flue-curing.","volume":"62","author":"Wada","year":"1956","journal-title":"Arch. Biochem. Biophys."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1021\/jf00032a030","article-title":"Biochemical and model chemical reactions for the basis of red pigment in flue-cured tobacco.","volume":"41","author":"Weeks","year":"1993","journal-title":"J. Agric. Food Chem."}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2022.861081\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T18:50:03Z","timestamp":1648407003000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2022.861081\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,22]]},"references-count":34,"alternative-id":["10.3389\/fpls.2022.861081"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2022.861081","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,22]]},"article-number":"861081"}}