{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T22:42:05Z","timestamp":1773700925026,"version":"3.50.1"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>Plants employ multi-layered immune system to fight against pathogen infections. Different receptors are able to detect the invasion activities of pathogens, transduce signals to downstream components, and activate defense responses. Among those receptors, nucleotide-binding domain leucine-rich repeat containing proteins (NLRs) are the major intracellular ones. CHILLING SENSITIVE 3 (CHS3) is an Arabidopsis NLR with an additional Lin-11, Isl-1 and Mec-3 (LIM) domain at its C terminus. The gain-of-function mutant, <jats:italic>chs3-2D<\/jats:italic>, exhibiting severe dwarfism and constitutively activated defense responses, was selected as a genetic background in this study for a forward genetic screen. A mutant allele of <jats:italic>hsp90.2<\/jats:italic> was isolated as a partial suppressor of <jats:italic>chs3-2D<\/jats:italic>, suggesting that HSP90 is required for CHS3-mediated defense signaling. In addition, HSP90 is also required for the autoimmunity of the <jats:italic>Dominant Negative (DN)-SNIPER1<\/jats:italic> and gain-of-function <jats:italic>ADR1-L2 D484V<\/jats:italic> transgenic lines, suggesting a broad role for HSP90 in NLR-mediated defense. Overall, our work indicates a larger contribution of HSP90 not only at the sensor, but also the helper NLR levels.<\/jats:p>","DOI":"10.3389\/fpls.2022.888449","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T08:41:13Z","timestamp":1654245673000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["HSP90 Contributes to chs3-2D-Mediated Autoimmunity"],"prefix":"10.3389","volume":"13","author":[{"given":"Junxing","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanwan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanbing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solveig","family":"van Wersch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2077","DOI":"10.1126\/science.1067747","article-title":"Regulatory role of SGT1 in early R gene-mediated plant defenses.","volume":"295","author":"Austin","year":"2002","journal-title":"Science"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2007","DOI":"10.1038\/sj.emboj.7601084","article-title":"Role of SGT1 in resistance protein accumulation in plant immunity.","volume":"25","author":"Azevedo","year":"2006","journal-title":"EMBO J."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1320","DOI":"10.1111\/nph.12760","article-title":"Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses.","volume":"202","author":"Bao","year":"2014","journal-title":"New Phytol."},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2011.00071","article-title":"Mutations in an atypical TIR-NB-LRR-LIM resistance protein confer autoimmunity.","volume":"2","author":"Bi","year":"2011","journal-title":"Front. 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