{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:46:51Z","timestamp":1791614811804,"version":"4.3.4"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["21-03593S"],"award-info":[{"award-number":["21-03593S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>General volatile anesthetic diethyl ether blocks sensation and responsive behavior not only in animals but also in plants. Here, using a combination of RNA-seq and proteomic LC\u2013MS\/MS analyses, we investigated the effect of anesthetic diethyl ether on gene expression and downstream consequences in plant <jats:italic>Arabidopsis thaliana<\/jats:italic>. Differential expression analyses revealed reprogramming of gene expression under anesthesia: 6,168 genes were upregulated, 6,310 genes were downregulated, while 9,914 genes were not affected in comparison with control plants. On the protein level, out of 5,150 proteins identified, 393 were significantly upregulated and 227 were significantly downregulated. Among the highest significantly downregulated processes in etherized plants were chlorophyll\/tetrapyrrole biosynthesis and photosynthesis. However, measurements of chlorophyll <jats:italic>a<\/jats:italic> fluorescence did not show inhibition of electron transport through photosystem II. The most significantly upregulated process was the response to heat stress (mainly heat shock proteins, HSPs). Using transgenic <jats:italic>A. thaliana<\/jats:italic> expressing <jats:italic>APOAEQUORIN<\/jats:italic>, we showed transient increase of cytoplasmic calcium level [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>cyt<\/jats:sub> in response to diethyl ether application. In addition, cell membrane permeability for ions also increased under anesthesia. The plants pre-treated with diethyl ether, and thus with induced HSPs, had increased tolerance of photosystem II to subsequent heat stress through the process known as cross-tolerance or priming. All these data indicate that diethyl ether anesthesia may partially mimic heat stress in plants through the effect on plasma membrane.<\/jats:p>","DOI":"10.3389\/fpls.2022.995001","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T07:43:29Z","timestamp":1662968609000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Diethyl ether anesthesia induces transient cytosolic [Ca2+] increase, heat shock proteins, and heat stress tolerance of photosystem II in Arabidopsis"],"prefix":"10.3389","volume":"13","author":[{"given":"Andrej","family":"Pavlovi\u010d","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Jak\u0161ov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Ku\u010derov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"\u0160pundov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"R\u00e1c","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Roudnick\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Mith\u00f6fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1007\/s11120-008-9331-0","article-title":"Heat stress: an overview of molecular responses in photosynthesis","volume":"98","author":"Allakhverdiev","year":"2008","journal-title":"Photosynth. 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Rep."},{"key":"ref6","first-page":"173","article-title":"Animal models for alcoholism: genetic strategies and neurochemical mechanisms","volume":"59","author":"Collins","year":"1993","journal-title":"Biochem. Soc. Symp."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"1794","DOI":"10.1021\/pr101065j","article-title":"Andromeda: a peptide search engine integrated into the MaxQuant environment","volume":"10","author":"Cox","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1163","DOI":"10.4161\/psb.21000","article-title":"Mimosa pudica, Dionaea muscipula and anesthetics","volume":"7","author":"De Luccia","year":"2012","journal-title":"Plant Signal. Behav."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1259","DOI":"10.1093\/jxb\/eru004","article-title":"Stress-induced electrolyte leakage: the role of K+-permeable channels and involvement in programmed cell death and metabolic adjustment","volume":"65","author":"Demidchik","year":"2014","journal-title":"J. Exp. Bot."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/S0176-1617(99)80042-X","article-title":"The chloroplast 22-ku heat-shock protein: a lumenal protein that associates with the oxygen evolving complex and protects photosystem II during heat stress","volume":"155","author":"Downs","year":"1999","journal-title":"J. Plant Physiol."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"999","DOI":"10.3389\/fpls.2015.00999","article-title":"Multi-level interactions between heat shock factors, heat shock proteins, and the redox system regulate acclimation to heat","volume":"6","author":"Driedonks","year":"2015","journal-title":"Front. Plant Sci."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"S72","DOI":"10.1038\/sj.bjp.0706441","article-title":"Molecular targets underlying general anesthesia","volume":"147","author":"Franks","year":"2006","journal-title":"Br. J. Pharmacol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1038\/310599a0","article-title":"Do general anesthetics act by competitive binding to specific receptors?","volume":"310","author":"Franks","year":"1984","journal-title":"Nature"},{"key":"ref15","first-page":"147","article-title":"The polyphasic rise of the chlorophyll a fluorescence (O-K-J-I-P) in heat stressed leaves","volume":"48","author":"Guiss\u00e9","year":"1995","journal-title":"Arch. Sci. 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A."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"2379","DOI":"10.1104\/pp.104.042408","article-title":"Gene expression profiling of the tetrapyrrole metabolic pathway in Arabidopsis with a mini-array system","volume":"135","author":"Matsumoto","year":"2004","journal-title":"Plant Physiol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1093\/jxb\/51.345.659","article-title":"Chlorophyll fluorescence\u2014a practical guide","volume":"51","author":"Maxwell","year":"2000","journal-title":"J. Exp. Bot."},{"key":"ref28","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1111\/j.1469-8137.2008.02682.x","article-title":"Shaping the calcium signature","volume":"181","author":"McAinsh","year":"2009","journal-title":"New Phytol."},{"key":"ref29","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1007\/BF01834479","article-title":"Zur Theorie der Alkoholnarkose","volume":"42","author":"Meyer","year":"1899","journal-title":"Arch. Exp. 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Anaesth."},{"key":"ref31","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1007\/s00425-006-0248-6","article-title":"Light and dark modulation of chlorophyll biosynthetic genes in response to temperature","volume":"224","author":"Mohanty","year":"2006","journal-title":"Planta"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"3788","DOI":"10.1101\/gad.12.24.3788","article-title":"Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators","volume":"12","author":"Morimoto","year":"1998","journal-title":"Genes Dev."},{"key":"ref33","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1038\/nature12478","article-title":"Glutamate receptor-like genes mediate leaf-to-leaf wound signals","volume":"500","author":"Mousavi","year":"2013","journal-title":"Nature"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"3355","DOI":"10.1093\/jxb\/erac089","article-title":"Molecular basis of priming-induced acquired tolerance to multiple abiotic stresses in plants","volume":"73","author":"Nair","year":"2022","journal-title":"J. Exp. Bot."},{"key":"ref35","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/s0378-4274(98)00177-5","article-title":"The effect of volatile anesthetics on light-induced phosphorylation in spinach chloroplasts","author":"Nakao","year":"1998","journal-title":"Toxicol. Lett."},{"key":"ref36","volume-title":"Studien \u00fcber die Narkose Zugleich ein Beitrag zur Allgemeinen Pharmakologie","author":"Overton","year":"1901"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1213\/ane.0b013e31817f6d40","article-title":"Induction of heat shock protein 70 and preconditioning by sevoflurane: a potent protective interaction against myocardial ischemia-reperfusion injury","volume":"107","author":"Pagel","year":"2008","journal-title":"Anesth. Analg."},{"key":"ref38","doi-asserted-by":"publisher","first-page":"323","DOI":"10.5423\/PPJ.RW.08.2015.0150","article-title":"Heat shock proteins: a review of the molecular chaperones for plant immunity","volume":"31","author":"Park","year":"2015","journal-title":"Plant Pathol. J."},{"key":"ref39","doi-asserted-by":"publisher","first-page":"13757","DOI":"10.1073\/pnas.2004259117","article-title":"Studies on the mechanism of general anesthesia","volume":"117","author":"Pavel","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref40","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1093\/aob\/mcz177","article-title":"Anaesthesia with diethyl ether impairs jasmonate signaling in the carnivorous plant Venus flytrap (Dionaea muscipula)","volume":"125","author":"Pavlovi\u010d","year":"2020","journal-title":"Ann. Bot."},{"key":"ref41","doi-asserted-by":"publisher","first-page":"W191","DOI":"10.1093\/nar\/gkz369","article-title":"G:profiler: a web server for functional enrichment analysis and conversions of gene lists (2019 update)","volume":"47","author":"Raudvere","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref42","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1007\/BF02172188","article-title":"A new puffing pattern induced by temperature shock and DNP in drosophila","volume":"18","author":"Ritossa","year":"1962","journal-title":"Experientia"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"2829","DOI":"10.1105\/tpc.108.065318","article-title":"The heat shock response in moss plants is regulated by specific calcium-permeable channels in the plasma membrane","volume":"21","author":"Saidi","year":"2009","journal-title":"Plant Cell"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"856","DOI":"10.4161\/psb.3.10.6021","article-title":"Investigating the role of plant heat shock proteins during oxidative stress","volume":"3","author":"Scarpeci","year":"2008","journal-title":"Plant Signal. Behav."},{"key":"ref45","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1038\/nprot.2006.4","article-title":"Tricine\u2013SDS-PAGE","volume":"1","author":"Sch\u00e4gger","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref46","doi-asserted-by":"publisher","first-page":"2851","DOI":"10.1038\/s41598-022-06915-z","article-title":"Ether anesthetics prevents touch-induced trigger hair calcium-electrical signals excite the Venus flytrap","volume":"12","author":"Scherzer","year":"2022","journal-title":"Sci. Rep."},{"key":"ref47","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1097\/00000542-200403000-00005","article-title":"Trigger-dependent gene expression profiles in cardiac preconditioning. Evidence for distinct genetic programs in ischemic and anesthetic preconditioning","volume":"100","author":"Sergeev","year":"2004","journal-title":"Anesthesiology"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/S0005-2728(97)00017-0","article-title":"Regulation of antenna structure and electron transport in photosystem II of Pisum sativum under elevated temperature probed by the fast polyphasic chlorophyll a fluorescence transient: OKJIP","volume":"1320","author":"Srivastava","year":"1997","journal-title":"Biochim. Biophys. Acta-Bioenerget."},{"key":"ref49","doi-asserted-by":"publisher","first-page":"16035","DOI":"10.1073\/pnas.252522299","article-title":"Large-scale molecular dynamics simulations of general anesthetic effects on the ion channel in the fully hydrated membrane: the implication of molecular mechanisms of general anesthesia","volume":"99","author":"Tang","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref02","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1104\/pp.117.3.851","article-title":"Temperature-stress-induced impairment of chlorophyll biosynthetic reactions in cucumber and wheat","volume":"117","author":"Tewari","year":"1998","journal-title":"Plant Physiol."},{"key":"ref50","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1126\/science.aat7744","article-title":"Glutamate triggers long-distance, calcium-based plant defense signaling","volume":"361","author":"Toyota","year":"2018","journal-title":"Science"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"9366","DOI":"10.1038\/s41598-020-66122-6","article-title":"Anesthetic-dependent changes in gene expression following acute and chronic exposure in the rodent brain","volume":"10","author":"Upton","year":"2020","journal-title":"Sci. Rep."},{"key":"ref52","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1093\/bja\/aef164","article-title":"Concepts and correlations relevant to general anaesthesia","volume":"89","author":"Urban","year":"2002","journal-title":"Br. J. Anaesth."},{"key":"ref53","doi-asserted-by":"publisher","first-page":"2479","DOI":"10.1073\/pnas.96.5.2479","article-title":"A neomorphic syntaxin mutation blocks volatile-anesthetic action in Caenorhabditis elegans","volume":"96","author":"van Swinderen","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"ref54","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/j.tibs.2007.06.009","article-title":"Can the stress protein response be controlled by \u2018membrane-lipid therapy\u2019?","volume":"32","author":"Vigh","year":"2007","journal-title":"Trends Biochem. Sci."},{"key":"ref55","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1007\/s11103-006-0045-4","article-title":"Heat stress-induced H(2)O(2) is required for effective expression of heat shock genes in Arabidopsis","volume":"61","author":"Volkov","year":"2006","journal-title":"Plant Mol. Biol."},{"key":"ref56","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal sample preparation method for proteome analysis","volume":"6","author":"Wi\u015bniewski","year":"2009","journal-title":"Nat. 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