{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T01:36:38Z","timestamp":1789176998617,"version":"build-2803163510"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T00:00:00Z","timestamp":1677715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2018R1D1A1B07043814"],"award-info":[{"award-number":["NRF-2018R1D1A1B07043814"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Plant Sci."],"abstract":"<jats:p>The most common symptom of iron (Fe) deficiency in plants is leaf chlorosis caused by impairment of chlorophyll biosynthesis. Magnesium (Mg)-chelatase H subunit (CHLH) is a key component in both chlorophyll biosynthesis and plastid signaling, but its role in Fe deficiency is poorly understood. Heterologous expression of the <jats:italic>Arabidopsis thaliana<\/jats:italic> Mg-chelatase H subunit gene (<jats:italic>AtCHLH<\/jats:italic>) increased Mg-chelatase activity by up to 6-fold and abundance of its product, Mg-protoporphyrin IX (Mg-Proto IX), by 60\u201375% in transgenic rice (<jats:italic>Oryza sativa<\/jats:italic>) seedlings compared to wild-type (WT) controls. Noticeably, the transgenic seedlings showed alleviation of Fe deficiency symptoms, as evidenced by their less pronounced leaf chlorosis and lower declines in shoot growth, chlorophyll contents, and photosynthetic efficiency, as indicated by <jats:italic>F<\/jats:italic><jats:sub>v<\/jats:sub>\/<jats:italic>F<\/jats:italic><jats:sub>m<\/jats:sub> and electron transport rate, compared to those in WT seedlings under Fe deficiency. Porphyrin metabolism was differentially regulated by Fe deficiency between WT and transgenic seedlings, particularly with a higher level of Mg-Proto IX in transgenic lines, showing that overexpression of <jats:italic>AtCHLH<\/jats:italic> reprograms porphyrin metabolism in transgenic rice. Leaves of Fe-deficient transgenic seedlings exhibited greater upregulation of deoxymugineic acid biosynthesis-related genes (i.e., <jats:italic>NAS<\/jats:italic>, <jats:italic>NAS2<\/jats:italic>, and <jats:italic>NAAT1<\/jats:italic>), <jats:italic>YSL2<\/jats:italic> transporter gene, and Fe-related transcription factor genes <jats:italic>IRO2<\/jats:italic> and <jats:italic>IDEF2<\/jats:italic> than those of WT, which may also partly contribute to alleviating Fe deficiency. Although <jats:italic>At<\/jats:italic>CHLH was postulated to act as a receptor for abscisic acid (ABA), exogenous ABA did not alter the phenotypes of Fe-deficient WT or transgenic seedlings. Our study demonstrates that modulation of porphyrin biosynthesis through expression of <jats:italic>AtCHLH<\/jats:italic> in transgenic rice alleviates Fe deficiency-induced stress, suggesting a possible role for CHLH in Fe deficiency responses.<\/jats:p>","DOI":"10.3389\/fpls.2023.1098808","type":"journal-article","created":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T06:10:11Z","timestamp":1677737411000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Expression of the Arabidopsis Mg-chelatase H subunit alleviates iron deficiency-induced stress in transgenic rice"],"prefix":"10.3389","volume":"14","author":[{"given":"Lien Hong","family":"Tran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Gil","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunyo","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,3,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1964","DOI":"10.1105\/tpc.106.048744","article-title":"In vivo visualization of A coordinator of photosynthetic gene expression in the nucleus and the chloroplast","volume":"19","author":"Ankele","year":"2007","journal-title":"Plant Cell"},{"key":"B2","first-page":"287","volume-title":"\u201cTetrapyrrole metabolism in photosynthetic organisms,\u201d in biosynthesis of heme and chlorophyll, ed. H.A. daily","author":"Beale","year":"1990"},{"key":"B3","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1007\/BF00033159","article-title":"Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence, and photosynthesis in leaves of Hedera canariensis","volume":"25","author":"Bilger","year":"1990","journal-title":"Photosynth Res."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.1093\/jxb\/erf004","article-title":"Cloning an iron-regulated metal transporter from rice","volume":"53","author":"Bughio","year":"2002","journal-title":"J. Exp. Bot."},{"key":"B5","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1007\/s11103-012-9965-3","article-title":"Roles of the different components of magnesium chelatase in abscisic acid signal transduction","volume":"80","author":"Du","year":"2012","journal-title":"Plant Mol. Biol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"5624","DOI":"10.1073\/pnas.93.11.5624","article-title":"A novel iron-regulated metal transporter from plants identified by functional expression in yeast","volume":"93","author":"Eide","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B7","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s00425-012-1757-0","article-title":"Shoot to root communication is necessary to control the expression of iron-acquisition genes in strategy I plants","volume":"237","author":"Garc\u00eda","year":"2013","journal-title":"Planta"},{"key":"B8","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1104\/pp.111.1.61","article-title":"A putative mg chelatase subunit from Arabidopsis thaliana cv C24. sequence and transcript analysis of the gene, import of the protein into chloroplasts, and in situ localization of the transcript and protein","volume":"111","author":"Gibson","year":"1996","journal-title":"Plant Physiol."},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.01997","article-title":"Ascorbate alleviates fe deficiency-induced stress in cotton (Gossypium hirsutum) by modulating ABA levels","volume":"7","author":"Guo","year":"2017","journal-title":"Front. Plant Sci."},{"key":"B10","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1080\/00380768.2022.2106115","article-title":"Elucidation of efficient photosynthesis in plants with limited iron","volume":"68","author":"Higuchi","year":"2022","journal-title":"Soil Sci. Plant Nutr."},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.01650","article-title":"CHLH\/GUN5 function in tetrapyrrole metabolism is correlated with plastid signaling but not ABA responses in guard cells","volume":"7","author":"Ibata","year":"2016","journal-title":"Front. Plant Sci."},{"key":"B12","doi-asserted-by":"publisher","first-page":"3470","DOI":"10.1074\/jbc.M806042200","article-title":"Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings","volume":"284","author":"Inoue","year":"2009","journal-title":"J. Biol. Chem."},{"key":"B13","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1111\/j.1365-313X.2010.04158.x","article-title":"Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese","volume":"62","author":"Ishimaru","year":"2010","journal-title":"Plant J."},{"key":"B14","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1111\/j.1365-313X.2005.02624.x","article-title":"Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+","volume":"45","author":"Ishimaru","year":"2006","journal-title":"Plant J."},{"key":"B15","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1111\/j.1365-313X.2011.04772.x","article-title":"The rice transcription factor IDEF1 directly binds to iron and other divalent metals for sensing cellular iron status","volume":"69","author":"Kobayashi","year":"2012","journal-title":"Plant J."},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1186\/s12284-014-0027-0","article-title":"Iron deficiency responses in rice roots","volume":"27","author":"Kobayashi","year":"2014","journal-title":"Rice 7"},{"key":"B17","doi-asserted-by":"publisher","first-page":"2792","DOI":"10.1038\/ncomms3792","article-title":"Iron-binding haemerythrin RING ubiquitin ligases regulate plant iron responses and accumulation","volume":"4","author":"Kobayashi","year":"2013","journal-title":"Nat. Commun."},{"key":"B18","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1146\/annurev-arplant-042811-105522","article-title":"Iron uptake, translocation, and regulation in higher plants","volume":"63","author":"Kobayashi","year":"2012","journal-title":"Annu. Rev. Plant Biol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.plantsci.2014.04.002","article-title":"Iron sensors and signals in response to iron deficiency","volume":"224","author":"Kobayashi","year":"2014","journal-title":"Plant Sci."},{"key":"B20","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.freeradbiomed.2018.10.439","article-title":"Iron transport and its regulation in plants","volume":"133","author":"Kobayashi","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1093\/aob\/mcq002","article-title":"The spatial expression and regulation of transcription factors IDEF1 and IDEF2","volume":"105","author":"Kobayashi","year":"2010","journal-title":"Ann. Bot."},{"key":"B22","doi-asserted-by":"publisher","first-page":"19150","DOI":"10.1073\/pnas.0707010104","article-title":"The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants","volume":"104","author":"Kobayashi","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B23","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1111\/j.1365-313X.2004.02146.x","article-title":"OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron and expressed in the phloem","volume":"39","author":"Koike","year":"2004","journal-title":"Plant J."},{"key":"B24","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1111\/j.1467-7652.2009.00469.x","article-title":"Carbon monoxide improves adaptation of arabidopsis to iron deficiency","volume":"8","author":"Kong","year":"2010","journal-title":"Plant Biotechnol. J."},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21093395","article-title":"Regulation of iron homeostasis and use in chloroplasts","volume":"21","author":"Kroh","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.01586","article-title":"Tetrapyrrole signaling in plants","volume":"7","author":"Larkin","year":"2016","journal-title":"Front. Plant Sci."},{"key":"B27","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1126\/science.1079978","article-title":"GUN4, a regulator of chlorophyll synthesis and intracellular signaling","volume":"299","author":"Larkin","year":"2003","journal-title":"Science"},{"key":"B28","doi-asserted-by":"publisher","first-page":"1134","DOI":"10.1104\/pp.99.3.1134","article-title":"Chloroplast biogenesis 65","volume":"99","author":"Lee","year":"1992","journal-title":"Plant Physiol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1093\/pcp\/41.6.743","article-title":"Transgenic rice plants expressing a Bacillus subtilis protoporphyrinogen oxidase gene are resistant to diphenyl ether herbicide oxyfluorfen","volume":"41","author":"Lee","year":"2000","journal-title":"Plant Cell Physiol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1111\/pce.12203","article-title":"Abscisic acid alleviates iron deficiency by promoting root iron reutilization and transport from root to shoot in arabidopsis","volume":"37","author":"Lei","year":"2014","journal-title":"Plant Cell Environ."},{"key":"B31","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1111\/j.1365-313X.2006.02894.x","article-title":"Reduced activity of plastid protoporphyrinogen oxidase causes attenuated photodynamic damage during high-light compared to low-light exposure","volume":"48","author":"Lermontova","year":"2006","journal-title":"Plant J."},{"key":"B32","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/0076-6879(87)48036-1","article-title":"Chlorophylls and carotenoids: pigments of photosynthetic biomembranes","volume":"148","author":"Lichtenthaler","year":"1987","journal-title":"Methods Enzymol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/S0021-9258(18)65809-0","article-title":"The occurrence and determination of \u03b4-aminolevuline acid and porphobilinogen in urine","volume":"219","author":"Mauzerall","year":"1956","journal-title":"J. Biol. Chem."},{"key":"B34","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.1073\/pnas.98.4.205","article-title":"Arabidopsis genomes uncoupled 5 (GUN5) mutant reveals the involvement of mg-chelatase h subunit in plastid-to-nucleus signal transduction","volume":"98","author":"Mochizuki","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B35","doi-asserted-by":"publisher","first-page":"886","DOI":"10.1104\/pp.97.3.886","article-title":"Chlorophyll fluorescence and photon yield of oxygen evolution in iron-deficient sugar beet (Beta vulgaris l.) leaves","volume":"97","author":"Morales","year":"1991","journal-title":"Plant Physiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1104\/pp.109.135277","article-title":"The barley magnesium chelatase 150-kD subunit is not an abscisic acid receptor","volume":"150","author":"M\u00fcller","year":"2009","journal-title":"Plant Physiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.plaphy.2007.03.021","article-title":"Expression profiling of Oryza sativa metal homeostasis genes in different rice cultivars using a cDNA macroarray","volume":"45","author":"Narayanan","year":"2007","journal-title":"Plant Physiol. Biochem."},{"key":"B38","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1111\/j.1365-313X.2007.03149.x","article-title":"The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in fe uptake under fe-deficient conditions","volume":"51","author":"Ogo","year":"2007","journal-title":"Plant J."},{"key":"B39","doi-asserted-by":"publisher","first-page":"13407","DOI":"10.1074\/jbc.M708732200","article-title":"A novel NAC transcription factor, IDEF2, that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants","volume":"283","author":"Ogo","year":"2008","journal-title":"J. Biol. Chem."},{"key":"B40","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1007\/s004250050558","article-title":"Expression studies in tetrapyrrole biosynthesis: inverse maxima of magnesium chelatase and ferrochelatase activity during cyclic photoperiods","volume":"208","author":"Papenbrock","year":"1999","journal-title":"Planta"},{"key":"B41","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1007\/s10535-015-0505-2","article-title":"Alterations in the porphyrin biosynthesis and antioxidant responses to chilling and heat stresses in Oryza sativa","volume":"59","author":"Phung","year":"2015","journal-title":"Biol. Plant"},{"key":"B42","doi-asserted-by":"publisher","first-page":"1746","DOI":"10.1104\/pp.111.188276","article-title":"Porphyrin biosynthesis control under water stress: sustained porphyrin status correlates with drought tolerance in transgenic rice","volume":"157","author":"Phung","year":"2011","journal-title":"Plant Physiol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1080\/01904168409363238","article-title":"The role of iron in higher-plant chlorophyll biosynthesis, maintenance and chloroplast biogenesis","volume":"7","author":"Pushnik","year":"1984","journal-title":"J. Plant Nutr."},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2013.00276","article-title":"The transcriptional response of arabidopsis leaves to fe deficiency","volume":"4","author":"Rodr\u00edguez-Celma","year":"2013","journal-title":"Front. Plant Sci."},{"key":"B45","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1104\/pp.81.4.965","article-title":"Biosynthesis of protoheme and heme a from glutamate in maize","volume":"81","author":"Schneegurt","year":"1986","journal-title":"Plant Physiol."},{"key":"B46","first-page":"49","volume-title":"\u201cChlorophyll fluorescence as a non-intrusive indicator for rapid assessment of in vivo photosynthesis,\u201d in ecophysiology of photosynthesis, ecological studies, eds","author":"Schreiber","year":"1994"},{"key":"B47","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1038\/nature05176","article-title":"The mg-chelatase h subunit is an abscisic acid receptor","volume":"443","author":"Shen","year":"2006","journal-title":"Nature"},{"key":"B48","doi-asserted-by":"publisher","first-page":"17816","DOI":"10.1074\/jbc.RA120.016181","article-title":"A novel method produces native light-harvesting complex II aggregates from the photosynthetic membrane revealing their role in nonphotochemical quenching","volume":"295","author":"Shukla","year":"2020","journal-title":"J. Biol. Chem."},{"key":"B49","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/j.freeradbiomed.02.034","article-title":"Heme oxygenase-nitric oxide crosstalk-mediated iron homeostasis in plants under oxidative stress","volume":"182","author":"Singh","year":"2022","journal-title":"Free Radic. Biol. Med."},{"key":"B50","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1104\/pp.69.1.107","article-title":"Effects of iron and oxygen on chlorophyll biosynthesis. i. In vivo observations on iron and oxygen-deficient plants","volume":"69","author":"Spiller","year":"1982","journal-title":"Plant Physiol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1038\/nature01204","article-title":"Chloroplast to nucleus communication triggered by accumulation of mg-protoporphyrinIX","volume":"421","author":"Strand","year":"2003","journal-title":"Nature"},{"key":"B52","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1146\/annurev.arplant.57.032905.105448","article-title":"Tetrapyrrole biosynthesis in higher plants","volume":"58","author":"Tanaka","year":"2007","journal-title":"Annu. Rev. Plant Biol."},{"key":"B53","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1104\/pp.65.1.114","article-title":"Limiting factors in photosynthesis. i. use of iron stress to control photochemical capacity in vivo","volume":"65","author":"Terry","year":"1980","journal-title":"Plant Physiol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"3695","DOI":"10.1093\/jxb\/erm219","article-title":"Proteomics, pigment composition, and organization of thylakoid membranes in iron-deficient spinach leaves","volume":"58","author":"Timperio","year":"2007","journal-title":"J. Exp. Bot."},{"key":"B55","volume-title":"Important roles of porphyrin biosynthesis in rice plants under various environmental conditions: iron deficiency and different light quality","author":"Tran","year":"2020"},{"key":"B56","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1007\/s10265-011-0426-x","article-title":"Mg-chelatase h subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana","volume":"124","author":"Tsuzuki","year":"2011","journal-title":"J. Plant Res."},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2013.00440","article-title":"Overexpression of the mg-chelatase h subunit in guard cells confers drought tolerance via promotion of stomatal closure in Arabidopsis thaliana","volume":"4","author":"Tsuzuki","year":"2013","journal-title":"Front. Plant Sci."},{"key":"B58","first-page":"31","volume-title":"\u201cEssential nutrients for plant growth: nutrient functions and deficiency symptoms\u201d, in plant nutrient management in hawaii\u2019s soils, approaches for tropical and subtropical agriculture","author":"Uchida","year":"2000"},{"key":"B59","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1104\/pp.102.016089","article-title":"Dual regulation of the arabidopsis high-affinity root iron uptake system by local and long-distance signals","volume":"132","author":"Vert","year":"2003","journal-title":"Plant Physiol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1105\/tpc.001388","article-title":"IRT1, an arabidopsis transporter essential for iron uptake from the soil and for plant growth","volume":"14","author":"Vert","year":"2002","journal-title":"Plant Cell"},{"key":"B61","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/j.tplants.2013.01.006","article-title":"Signals from chloroplasts and mitochondria for iron homeostasis regulation","volume":"18","author":"Vigani","year":"2013","journal-title":"Trends Plant Sci."},{"key":"B62","doi-asserted-by":"publisher","first-page":"1940","DOI":"10.1104\/pp.109.140731","article-title":"The magnesium-chelatase h subunit binds abscisic acid and functions in abscisic acid signaling: new evidence in arabidopsis","volume":"150","author":"Wu","year":"2009","journal-title":"Plant Physiol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"2130","DOI":"10.1104\/pp.109.152728","article-title":"Transcriptional profiling of the arabidopsis iron deficiency response reveals conserved transition metal homeostasis networks","volume":"152","author":"Yang","year":"2010","journal-title":"Plant Physiol."},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2019.109018","article-title":"Abscisic acid alleviates iron deficiency by regulating iron distribution in roots and shoots of apple","volume":"262","author":"Zhang","year":"2020","journal-title":"Sci. Hortic."},{"key":"B65","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1016\/j.jplph.2010.10.016","article-title":"Transient accumulation of mg-protoporphyrin IX regulates expression of PhANGs \u2013 new evidence for the signaling role of tetrapyrroles in mature arabidopsis plants","volume":"168","author":"Zhang","year":"2011","journal-title":"J. Plant Physiol."}],"container-title":["Frontiers in Plant Science"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2023.1098808\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T06:10:17Z","timestamp":1677737417000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fpls.2023.1098808\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,2]]},"references-count":65,"alternative-id":["10.3389\/fpls.2023.1098808"],"URL":"https:\/\/doi.org\/10.3389\/fpls.2023.1098808","relation":{},"ISSN":["1664-462X"],"issn-type":[{"value":"1664-462X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,2]]},"article-number":"1098808"}}